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Plasmid#193755PurposeExpresses LuxR in forward direction under the control of BBa_J23102 constitutive promoter, ColE1 origin of replication, Ampicillin selectionDepositorInsertLuxR
UseTagsExpressionBacterialMutationPromoterBBa_J23102Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDA313
Plasmid#195214PurposeExpression of superfolder GFP (sfGFP) by a hybrid T7 promoter with tetO.DepositorInsertsfGFP with tetO and a hairpin
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKP925
Plasmid#167591PurposeExpress TrpC-TonB fusion proteinDepositorInserttrpC-tonB
UseTagsExpressionBacterialMutationResidues 1-25 TrpC; Residues 26-31 Linker; Residu…PromoterAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP933
Plasmid#167657PurposeExpress colicinB-His6, V21C, M261CDepositorInsertcba-his6 V21C, M261C
UseTagsExpressionBacterialMutationcba-his6 with V21C, M261C mutationsPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYFP-lacY-10
Plasmid#140139PurposeEvaluating the impact of feedback and high sensor concentration on information transmission of a genetic sensor.DepositorInsertlacY (lacY )
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGC682
Plasmid#107020PurposeGFP reporter of lag-2 driven by 1 kb of promoter, which lackes DBS motifDepositorInsertGFP-PH
UseTagsGFPExpressionWormMutationPromoterlag-2Available sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGC681
Plasmid#107019PurposeGFP reporter of lag-2 driven by 405 bp of promoterDepositorInsertGFP-PH
UseTagsGFPExpressionWormMutationPromoterlag-2Available sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGC643
Plasmid#107016PurposeGFP reporter of lag-2 driven by 0.5 kb promoterDepositorInsertGFP-PH
UseTagsGFPExpressionWormMutationPromoterlag-2Available sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAS_gpyrG2
Plasmid#90277Purpose(also pMST620-BB3_gpyrG2_cas9) CRISPR/Cas9 plasmid with gRNA for site pyrG2, Cas9DepositorInsertgRNA (pyrg2)
UseA. nigerTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMST1211_BB2_E_AB_pcoxA:pyrGtrunc_AMA1_2.8
Plasmid#90285PurposeBB2 with split pyrG marker for homologous integration with linker AB (negative control)DepositorInsertpyrGtrunc
UseTagsExpressionBacterialMutationtruncated version of pyrG gene (sequence is from …PromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMST1212_BB2_E_BC_pcoxA:pyrGtrunc_AMA1_2.8
Plasmid#90286PurposeBB2 with split pyrG marker for homologous integration with linker BC (for 1 expression cassette)DepositorInsertpyrGtrunc
UseTagsExpressionBacterialMutationtruncated version of pyrG gene (sequence is from …PromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMST1213_BB2_E_CD_pcoxA:pyrGtrunc_AMA1_2.8
Plasmid#90287PurposeBB2 with split pyrG marker for homologous integration with linker CD (for 2 expression cassettes)DepositorInsertpyrGtrunc
UseTagsExpressionBacterialMutationtruncated version of pyrG gene (sequence is from …PromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMST1214_BB2_E_DE_pcoxA:pyrGtrunc_AMA1_2.8
Plasmid#90288PurposeBB2 with split pyrG marker for homologous integration with linker DE (for 3 expression cassettes)DepositorInsertpyrGtrunc
UseTagsExpressionBacterialMutationtruncated version of pyrG gene (sequence is from …PromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
sankE3_5
Plasmid#24893DepositorInsertsank E3_5
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
PrbC5
Plasmid#24787DepositorInsertPrb
UseTagsHisExpressionBacterialMutationAsp11Gly, Asp83Asn, Val92Ala, Glu119Gly, and Glu1…PromoterAvailable sinceJuly 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
BS.YxiN.Twin1.a1-368.wt
Plasmid#13528DepositorInsertB. subtilis DEAD-box helicase YxiN first two domains
UseTagsintein-chitin binding domainExpressionBacterialMutationdeleted amino acid residues 369-479PromoterAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
BS.YxiN.Twin1.a404-479.wt
Plasmid#13529DepositorInsertB. subtilis DEAD-box helicase YxiN specific RNA binding domain
UseTagsintein-chitin binding domainExpressionBacterialMutationdelete amino acid residues 1-403PromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJC45BS104 Y257W
Plasmid#1303DepositorInsertHsp104 Y257W (HSP104 Budding Yeast)
UseTags10X His tagExpressionBacterialMutationChanged Tyr 257 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJC45BS104 Y507W
Plasmid#1305DepositorInsertHsp104 Y507W (HSP104 Budding Yeast)
UseTags10X His tagExpressionBacterialMutationChanged Tyr 507 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGALSc104b F130W
Plasmid#1309DepositorInsertHsp104 F130W (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationChanged Phe 130 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only