We narrowed to 5,510 results for: pcas

1. Tubp-Gal80 in pCaSpeR4

   Plasmid

   #17748

   Depositor

   Liqun Luo

   Article

   Lee et al Neuron. 1999 Mar . 22(3):451-61.

   Insert

   GAL80 (GAL80 Budding Yeast)
   

   Use

   Tags

   SV40 polyA and Tubulin 1alpha promoter

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAX-mScarlet-CIP4

   Plasmid

   #178364

   Purpose

   Expresses CIP4 c-terminal labeled with mScarlet in mammalian cells

   Depositor

   Erik Dent

   Article

   Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

   Insert

   Cdc42 Interacting Protein 4 (TRIP10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG mEmerald-LAMP1

   Plasmid

   #168513

   Purpose

   Expression of rat LAMP1 fused with mEmerald to perform live tracking of lysosomes

   Depositor

   Franck Polleux

   Article

   Courchet et al Cell. 2013 Jun 20;153(7):1510-25. doi: 1

   Insert

   LAMP1 (Lamp1 Rat)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. DHFR-SpCas9-DHFR

   Plasmid

   #85447

   Purpose

   Expresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

   Insert

   DHFR-SpCas9-DHFR
   

   Use

   AAV

   Tags

   Destabilized domain (C-terminus version) of E.col…

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-pCaMKII-tTAc

   Plasmid

   #172125

   Purpose

   Expression of InteinC-tTAC in CaMKII positive cells

   Depositor

   Chun Xu

   Article

   Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

   Insert

   pCaMKII, tTAc
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CaMKII

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG/ATP11A(E186Q)-HA

   Plasmid

   #209249

   Purpose

   Mammalian expression of ATP11A mutant E186Q

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

   Insert

   ATP11A (ATP11A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E186Q

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-ChrimsonR-mRuby2-ST

   Plasmid

   #105447

   Purpose

   Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

   Insert

   ChrimsonR-mRuby2-ST
   

   Use

   Tags

   mRuby2 and soma targeting motif

   Expression

   Mammalian

   Mutation

   K176R in Chrimson

   Promoter

   pCAGGS

   Available since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-HA-Nrxn1beta AS4(+)

   Plasmid

   #59410

   Purpose

   Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]

   Depositor

   Peter Scheiffele

   Article

   Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

   Insert

   Nrxn1beta AS4(+) (Nrxn1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-hPPP3CA-FLAG

   Plasmid

   #179134

   Purpose

   Expression vector of human protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

   Depositor

   Masahito Ikawa

   Article

   Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

   Insert

   protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS puro HA-hUSP27X

    Plasmid

    #225718

    Purpose

    Bacterial expression of USP27X with HA tag

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    USP27X (USP27X Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-ChroME-mRuby2-ST

    Plasmid

    #108902

    Purpose

    Cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    ChroME-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. modified pcAT7-Glo1

    Plasmid

    #160996

    Purpose

    Splicing reporter backbone

    Depositor

    Brenton Graveley

    Article

    Adamson et al Genome Biol. 2018 Jun 1;19(1):71. doi: 1

    Insert

    hemoglobin subunit beta (HBB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Repeated intron 1 with 40 nucelotide deletion

    Promoter

    CAG

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAS9-mCherry empty

    Plasmid

    #80975

    Purpose

    Cas9, mCherry, and sgRNA expression

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-SpCas9-HF1

    Plasmid

    #108301

    Purpose

    pX330 (Plasmid #42230) with the N497A, R661A, Q695A, and Q926A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-ChRmine-mRuby2-ST

    Plasmid

    #227777

    Purpose

    Cation channelrhodopsin ChRmine fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChRmine-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG mito-mTagBFP2

    Plasmid

    #105011

    Purpose

    Mitochondrial marker

    Depositor

    Franck Polleux

    Article

    Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

    Insert

    mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-ChR2-mRuby2-ST

    Plasmid

    #109125

    Purpose

    Cation channelrhodopsin ChR2 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    ChR2-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCag FlpO-2A-Cre EV

    Plasmid

    #129419

    Purpose

    episomal expression of FlpO and Cre recombinases

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    FlpO-2A-Cre
    

    Use

    Cre/Lox and Unspecified; Episomal expression vect…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/Chick β-actin (CAG)

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. G1333 DddAtox-C–dSpCas9

    Plasmid

    #157834

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    bpNLS–G1333 DddAtox-C–dSpCas9–UGI–UGI–bpNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-GluA2-Fab-LC

    Plasmid

    #230063

    Purpose

    Expresses light chain Fab fragment of anti-GluA2. Can be used with heavy chain to produce Fab in HEK cells.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA2 Fab light chain fragment
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin (CAG)

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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