We narrowed to 10,632 results for: t7

1. pSB4k5_RNAnano (r-plasmid)

   Plasmid

   #127634

   Purpose

   Encodes an RNA nanostructure with MG, PP7, TAR and S1m RNA aptamers. Transcription via T7 RNAP.

   Depositor

   Friedrich Simmel

   Article

   Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.

   Insert

   RNA nanostrcutre
   

   Use

   Synthetic Biology

   Promoter

   T7 RNAP

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. DT043A

   Plasmid

   #159404

   Purpose

   Constitutive superfolder GFP (sfGFP) expression, controlled under T7 bacteriophage promoter

   Depositor

   Sheref Mansy

   Article

   Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

   Insert

   pr.T7-sfGFP
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Bacterial

   Promoter

   T7 bacteriophage promoter

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEU3-NII-GLICNot

   Plasmid

   #140182

   Purpose

   Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, GST-tag and NotI site

   Depositor

   Tamás Mészáros

   Article

   Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

   Type

   Empty backbone

   Use

   Expression vector for a cell-free system

   Tags

   GST

   Promoter

   T7

   Available Since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS1

   Plasmid

   #55749

   Purpose

   Produces aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

   Depositor

   Matthew Bennett

   Article

   Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

   Insert

   KanR
   

   Expression

   Bacterial

   Promoter

   T7-lac

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETA_21 tRNA genes

   Plasmid

   #242431

   Purpose

   Expresses 21 tRNAs. The plasmid contains a T7 promoter upstream and an Nt.BspQI recognition site as well as a T7 terminator downstream of each tRNA gene.

   Depositor

   Sebastian Maerkl

   Article

   Li et al Nat Commun. 2025 Jul 5;16(1):6212. doi:

   Insert

   21 tRNA genes
   

   Expression

   Bacterial

   Available Since

   Aug. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRII-mogat3b in situ probe

   Plasmid

   #232136

   Purpose

   zebrafish mogat3b anti-sense in situ probe, linearize with BamHI, T7 polymerase

   Depositor

   Steven Farber

   Article

   Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

   Insert

   mogat3b
   

   Use

   In vitro transcription

   Promoter

   SP6/T7

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSETA-qmTQ2-glucose

   Plasmid

   #225252

   Purpose

   Bacterial expression of N-terminal His-tagged qmTQ2-glucose, a cyan fluorescent FLIM biosensor for detecting glucose

   Depositor

   Yasushi Okada

   Article

   Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

   Insert

   qmTQ2-glucose
   

   Tags

   6XHis, T7 tag, XpressTM tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSETA-qmTQ2-cAMP

   Plasmid

   #225249

   Purpose

   Bacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

   Depositor

   Yasushi Okada

   Article

   Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

   Insert

   qmTQ2-cAMP
   

   Tags

   6XHis, T7 tag, XpressTM tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDL25_Cas9-His Δcys E1207C

   Plasmid

   #206286

   Purpose

   Bacterial expression of Cas9-His Δcys E1027C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, C574S, E1207C

   Promoter

   T7

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDL23_Cas9-His Δcys E945C

    Plasmid

    #206284

    Purpose

    Bacterial expression of Cas9-His Δcys E945C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    C80S, C574S, E945C

    Promoter

    T7

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDL24_Cas9-His Δcys E1026C

    Plasmid

    #206285

    Purpose

    Bacterial expression of Cas9-His Δcys E1026C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    C80S, C574S, E1026C

    Promoter

    T7

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDL22_Cas9-His Δcys E532C

    Plasmid

    #206283

    Purpose

    Bacterial expression of Cas9-His Δcys E532C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    C80S, E532C, C574S

    Promoter

    T7

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET23PI-Sce ESARC D218A

    Plasmid

    #195608

    Purpose

    Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D218A active site mutation

    Depositor

    Frederick Gimble

    Article

    Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

    Insert

    PI-SceI
    

    Expression

    Bacterial

    Mutation

    T928C, T929C, A942C

    Promoter

    T7

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7PI-Sce ESARC D326A

    Plasmid

    #195609

    Purpose

    Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D326A active site mutation

    Depositor

    Frederick Gimble

    Article

    Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

    Insert

    PI-SceI
    

    Expression

    Bacterial

    Mutation

    T3593C, A3601C, T3602G

    Promoter

    T7

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET23PI-Sce ESARC

    Plasmid

    #195607

    Purpose

    Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter

    Depositor

    Frederick Gimble

    Article

    Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

    Insert

    PI-SceI
    

    Expression

    Bacterial

    Mutation

    C552T,C554A, A557T,G584T, C588A, G878T, C881T, C9…

    Promoter

    T7

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEU3-NII-HxHLICNot

    Plasmid

    #140183

    Purpose

    Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, 12xHis-tag and NotI restriction site

    Depositor

    Tamás Mészáros

    Article

    Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

    Type

    Empty backbone

    Use

    Expression vector for a cell-free system

    Tags

    12xHis

    Promoter

    T7

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19_TTE1564_UUCG-loop

    Plasmid

    #133581

    Purpose

    T7-transcription template for a P2-deletion mutation of the Tte preQ1- riboswitch

    Depositor

    Nils Walter

    Article

    Lund et al Nucleic Acids Res. 2019 Dec 13. pii: 567

    Insert

    P2-deletion mutant of Tte preQ1-riboswitch
    

    Use

    Unspecified

    Mutation

    replacement of riboswitch nucleotides U6 through …

    Promoter

    T7

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSET OCaMP

    Plasmid

    #224932

    Purpose

    Expresses OCaMP, an orange calcium indicator, in bacteria. Contains T7 promoter and RSET leader sequence peptide (His tag, T7 leader, Xpress epitope)

    Depositor

    Alexander Lohman

    Article

    Aggarwal et al bioRxiv 2025.07.28.667269; doi: https://

    Insert

    OCaMP
    

    Tags

    6xHis, RSET peptide, and Xpress tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 28, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pXT7

    Plasmid

    #32995

    Depositor

    Sergei Sokol

    Type

    Empty backbone

    Use

    Xenopus rna expression

    Tags

    globin 3'UTR and globin 5' UTR

    Promoter

    T7

    Available Since

    Jan. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

20. VR124 pETv2 10His-pG-Tn5 (E54K, L372P)

    Plasmid

    #198468

    Purpose

    E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.

    Depositor

    Viviana Risca

    Article

    Soroczynski et al bioRxiv 2024.07.11.602973

    Insert

    10His-pG-Tn5 (E54K, L372P)
    

    Tags

    10His-pG

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    Consensus φ10 T7 Promoter

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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