159,557 results

1. BFP

   Plasmid

   #201383

   Purpose

   Control nucleocytoplasmic transport probe

   Depositor

   Pere Roca-Cusachs

   Article

   Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

   Insert

   BFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3 Control Vector

   Plasmid

   #212937

   Purpose

   Control vector with constitutive firefly luciferase expression

   Depositor

   Debrya Groskreutz

   Insert

   luc+
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   WT

   Promoter

   SV40

   Available since

   Jan. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. TRUPATH Triple Gaq

   Plasmid

   #196058

   Purpose

   Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphaQ-RLuc8 (GNAQ Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJL-TRBO

   Plasmid

   #80082

   Purpose

   An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence.

   Depositor

   John Lindbo

   Article

   Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSIP403

   Plasmid

   #122028

   Purpose

   Plasmid for expression of gene of interest in Lactobacillus plantarum and L. sakei

   Depositor

   Lars Axelsson, Geir Mathiesen

   Article

   Sorvig et al Microbiology. 2005 Jul;151(Pt 7):2439-49

   Insert

   gusA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   sppA

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. BPK764

   Plasmid

   #65767

   Purpose

   Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (x2)

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. His-FGE

   Plasmid

   #75147

   Purpose

   Expression vector for His(6) tagged Formylglycine Generating Enzyme (FGE) in bacterial host.

   Depositor

   Richard Fishel

   Article

   Liu et al Sci Rep. 2015 Nov 19;5:16883. doi: 10.10

   Insert

   FGE
   

   Use

   Tags

   Histidine tag and Thrombin

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   T7

   Available since

   July 8, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pLV-tTRKRAB

   Plasmid

   #12249

   Depositor

   Didier Trono

   Article

   Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

   Insert

   TetR-KRAB fusion
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSETa mEos2

   Plasmid

   #20341

   Purpose

   bacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)

   Depositor

   Loren Looger

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   mEos-2
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223_IDH1_p.R132C

    Plasmid

    #81726

    Purpose

    Gateway Donor vector containing IDH1, part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    IDH1 (IDH1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    R132C

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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