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Plasmid#41071DepositorInsertTetherin (BST2 Human)
UseTagsFLAGExpressionMammalianMutation74S, 84SPromoterCMVAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-neo-C11orf51-3xFlag (2765)
Plasmid#39859DepositorInsertC11orf51 (ANAPC15 Human)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
mPapaya-Farnesyl-5
Plasmid#56643PurposeLocalization: Membrane, Excitation: 530, Emission: 541DepositorInsertFarnesyl (HRAS Human)
UseTagsmPapayaExpressionMammalianMutationencodes final 20 aa of NM_001130442.1PromoterCMVAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGFP AH2 tensin
Plasmid#50514PurposeThis plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertTensin (TNS1 Chicken)
UseTagsGFPExpressionMammalianMutationAA659--761, including actin homology 2 domain of …PromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-mCherry-hPMCA2x/b
Plasmid#111569PurposeExpresses a plasma membrane calcium pump hPMCA2x/bDepositorInsertPlasma membrane calcium pump 2, variant x/b (ATP2B2 Human)
UseTagsmCherryExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgRNA_Dicer_1
Plasmid#68807Purposespecific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.DepositorInsertsgRNA mouse Dicer1
UseCRISPR and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Mfsd10
Plasmid#120238PurposeExpresses V5-tagged Mfsd10 in lentiviral vectorDepositorInsertMfsd10 (Mfsd10 Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-GEI-17(133-509)
Plasmid#87056PurposeExpresses 6xHis-tagged gei-17 (isoform f), with a TEV protease cleavage site between the tag and the ORFDepositorInsertGEI-17 isfoomr f (aa 133-509) (gei-17 Nematode)
UseTags6xHis-TEV and GAGGGS linkerExpressionBacterialMutationPromoterT7Available sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRFAM7ADelta2bp-mCherry
Plasmid#62638Purposemammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with mCherry fused into M3-M4 loopDepositorInsertCHRFAM7A (CHRFAM7A Human)
UseTagsExpressionMammalianMutationthere is a 2-bp deletion in exon 6 of CHRFAM7A an…PromoterCMVAvailable sinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
R0063 (pLuxR-pR)_AB
Plasmid#66012PurposeMoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [A:R0063:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC39A5
Plasmid#132057PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC39A5 (SLC39A5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti-CMV-cGASΔN C396/7A-HA
Plasmid#130918PurposeExpresses human cGASΔN (aa160-522)-HA with C396/397A point mutation; Puromycin selection markerDepositorInsertcGASΔN C396/7A
UseTagsHAExpressionMammalianMutationmutated residues 396 and 397 to alaninesPromoterCMVAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Halo-Tev- HSPB7 (zebrafish) C49S and C117S
Plasmid#108913PurposeExpress C49S/C117S double mutant of Zebrafish Halo-Tev-HSPB7 in mammalian cellsDepositorInsertHalo-TEV-HSPB7
UseTagsHalo-TEVExpressionMammalianMutationCysteine 49 to Serine, Cysteine 117 to SerinePromoterSP6Available sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1 hSlp4-a V21A
Plasmid#40064DepositorInserthSlp4-a V21A (SYTL4 Human)
UseTagsGSTExpressionBacterialMutationValine 21 to AlaninePromoterTacAvailable sinceOct. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIDS-RESTmut full-length
Plasmid#109162PurposeEncodes human full-length REST/NRSF C402S to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length REST/NRSF (REST Human)
UseTagsExpressionInsectMutationCys402SerPromoterp10Available sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMAL c2x Tral FL
Plasmid#113012PurposeFor bacterial expression of MBP fusion of full-length Drosophila Tral WTDepositorInsertfull length tral (tral Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pUC19-D4J3S7
Plasmid#103096PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertD4J3S7(Cas9 coding gene from Coprococcus catus GD/7)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
TXNDC-bio-His
Plasmid#51858PurposeExpresses full-length Thioredoxin-related transmembrane protein 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTXNDC (TMX1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-puro/hFOXH1 shRNA3
Plasmid#59308PurposeKnockdown of human FOXH1DepositorInserthFOXH1 shRNA (FOXH1 Human)
UseRNAi and RetroviralTagsExpressionMutationPromoterhU6Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only