164,826 results

1. pCAG mito-mEos2

   Plasmid

   #168515

   Purpose

   Expression of photoconvertible mEos2 targted to mitochondrial matrix to allow tracking of individual mitochondria and measurement of fission-fusion rate

   Depositor

   Franck Polleux

   Article

   Lewis TL et al Nat Commun. 2018 Nov 27;9(1):5008. doi:

   Insert

   mito-mEos2
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTA7001-DEST

   Plasmid

   #71745

   Purpose

   GATEWAY compatible derivative of plant binary vector pTA7001 from Nam Chua's laboratory at The Rockefeller University, Dexamethasone inducible

   Depositor

   Roger Innes

   Article

   Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   GAL4

   Available Since

   Feb. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-FGFR1c-V5/HIS

   Plasmid

   #201106

   Purpose

   expression of human FGFR1 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human FGFR1 receptor tyrosine kinase, variant c, full length, wildtype (FGFR1 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. p5E-hPGK

   Plasmid

   #82579

   Purpose

   5' entry vector containing human phosphoglycerate kinase (hPGK) promoter for ubiquitous expression

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   Human PGK promoter
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   None

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCIG3_mCherry_Rab5_DN

   Plasmid

   #75111

   Purpose

   expression of mCherry-Rab5 DN and EGFP

   Depositor

   Felicia Goodrum

   Insert

   RAB5A (RAB5A Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   DN S34N

   Promoter

   CMV

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-LRRK2

   Plasmid

   #232872

   Purpose

   Expresses LRRK2 in mammalian cells

   Depositor

   Pietro De Camilli

   Article

   Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

   Insert

   GFP-LRRK2 (LRRK2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLY092b-lenti-EFS-BCMA-BBz-T2APRODH2-T2APuro-WPRE

   Plasmid

   #192193

   Purpose

   Lenti-BCMA-CAR-PRODH2

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   Lenti-BCMA-CAR-PRODH2 (TNFRSF17 Synthetic)
   

   Use

   Lentiviral; Mammalian expression

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpdCas9 VPR

   Plasmid

   #188509

   Purpose

   Expresses FLAG tagged SpdCas9 VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. EFS-GFP

   Plasmid

   #110834

   Purpose

   positive control for GFP expression

   Depositor

   Michael Lewis

   Article

   Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

   Insert

   elongation factor 1alpha binding sequence (EFS) at GFP promoter region
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3036 GST-HPV-16 E6

    Plasmid

    #10849

    Depositor

    Peter Howley

    Article

    Huibregtse et al EMBO J. 1991 Dec . 10(13):4129-35.

    Insert

    HPV16 E6 (E6 Human papillomavirus)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pRVL-1

    Plasmid

    #104579

    Purpose

    Contains mouse kappa CL domain, for cloning of antibody VL domains using SapI restriction enzyme.

    Depositor

    Daniel Christ

    Article

    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

    Insert

    Mouse CL kappa
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lyn-R-GECO1

    Plasmid

    #120410

    Purpose

    Expresses a fusion between myristoylation signal peptide and R-GECO1. This construct targets R-GECO1 to the plasma membrane.

    Depositor

    Won Do Heo

    Article

    Kim et al Proc Natl Acad Sci U S A. 2016 May 24;11

    Insert

    Lyn-R-GECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO-Tet-On-YAP1-shRNA1

    Plasmid

    #176846

    Purpose

    lentiviral vector for doxycycline-inducible human YAP1 knockdown

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    YAP1-Sh1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCFD5_w

    Plasmid

    #112645

    Purpose

    Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.

    Depositor

    Michael Boutros

    Insert

    U6:3-tRNA-sgRNA expression cassette
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Reb_1 aka PAS619

    Plasmid

    #71224

    Purpose

    Expresses wild type R bodies from C. taeniospiralis in pETM11 backbone

    Depositor

    Pamela Silver

    Article

    Polka et al ACS Synth Biol. 2016 Feb 3.

    Insert

    Reb locus
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    RebA G53A

    Promoter

    T7

    Available Since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-SMVP-Cas9N

    Plasmid

    #80930

    Purpose

    Expresses Cas9N in mammalian cells.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. hCas9_D10A

    Plasmid

    #41816

    Purpose

    Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    Cas9_D10A
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A nickase

    Promoter

    CMV

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pInducer10b-EGFP-KRAS G12V

    Plasmid

    #164925

    Purpose

    Expresses EGFP cDNA and KRAS G12V cDNA in mammalian cells

    Depositor

    Ji Luo

    Article

    Carver et al PLoS One. 2014 Aug 5;9(8):e103836. doi:

    Inserts

    Enhanced green fluorescent protein
    KRAS proto-oncogene, GTPase (KRAS) G12V mutant (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Glycine 12 changed to valine (G->T 3156)

    Promoter

    Ubc Promoter and Ubc promoter

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFV2-HA

    Plasmid

    #187082

    Purpose

    Expression of ATP synthase, with all Cys changed to Ala, and subunit a-HA tagged, subunit beta-His tagged)

    Depositor

    Steven Vik

    Article

    Bae et al Biochim Biophys Acta. 2009 Sep;1787(9):1

    Insert

    atpBEFHAGDC
    

    Use

    Tags

    6-His beta subunit (atpD) and HA tag subunit a (a…

    Expression

    Bacterial

    Mutation

    All Cys codons changed to Ala.

    Promoter

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. CYP51A1 (3I3K)

    Plasmid

    #25161

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CYP51A1_cw_09 (CYP51A1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acid residues 54-502

    Promoter

    Available Since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits