We narrowed to 17,163 results for: EMB

Showing: 10601 - 10620 of 17163 results

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pcDNA3.1-CHRFAM7A-YFP

Plasmid

#62634

Purpose

mammalian expression of human duplicated Alpha7 (CHRFAM7A) with YFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Expression

Mammalian

Mutation

YFP inserted in M3-M4 loop

Promoter

CMV

Available Since

March 10, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-Ago2

Plasmid

#74231

Purpose

GFP-Ago2 lentiviral vector

Depositor

Carl Novina

Article

Janas et al RNA. 2012 Nov;18(11):2041-55. doi: 10.12

Insert

Ago2 (AGO2 Human)

Use

Lentiviral

Tags

eGFP

Available Since

April 6, 2016

Availability

Academic Institutions and Nonprofits only
mEosEM-E_Lamin A

Plasmid

#226338

Purpose

Photoswitchable fluorescent protein for correlative light-electron microscopy; nuclear envelope-targeted mEosEM-E for mammalian expression

Depositor

Pingyong Xu

Article

Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

Insert

Lamin A (LMNA Human)

Tags

mEosEM-E

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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Mini-Ii-Str_SBP-EGFP-LIMP2

Plasmid

#222324

Purpose

Synchronize the trafficking of LIMP2 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LIMP2 fused to SBP-EGFP (SCARB2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Arf6 T27N- Venus in pcDNA3.1

Plasmid

#90464

Purpose

RhoA mediated cell migration

Depositor

Narasimhan Gautam

Article

Castillo-Badillo et al ACS Synth Biol. 2020 Mar 16. doi: 10.102

Insert

Arf6 T27N (ARF6 )

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pME-mScarlet3-Giantin (JDW 1356)

Plasmid

#224502

Purpose

Gateway compatible middle entry clone containing a V5-tagged mScarlet fused to Giantin (For visualizing the Golgi)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mScarlet3-Giantin

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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HA-mRIN2 dRA

Plasmid

#108941

Purpose

Mammalian expression of mRIN2 dRA

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Deletion of RA domain (aa 751-836)

Promoter

CMV

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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HA-mRIN2 dRHVPS9

Plasmid

#108942

Purpose

Mammalian expression of mRIN2 dRHVPS9

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Deletion of RHVPS9 domain (aa 455-738)

Promoter

CMV

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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aav-tnt-actn2-gfp

Plasmid

#165034

Purpose

AAV-based delivery of ACTN2-GFP into cardiomyocytes in vivo

Depositor

William Pu

Article

Guo et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

Insert

ACTN2 (Actn2 Mouse)

Use

AAV

Tags

GFP

Promoter

cTNT

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-alpha-E-Catenin 3xFP4 (895)

Plasmid

#67936

Purpose

Mammalian expression of alpha E catenin

Depositor

Alpha Yap

Article

Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

Insert

alpha E catenin (Ctnna1 Mouse)

Tags

3xFP4 and EGFP

Expression

Mammalian

Mutation

N-terminal domain

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
pGEMHE-mClover3-KASH5-DN

Plasmid

#171492

Purpose

T7 promotor drives in vitro transcription of mClover3-tagged bovine Kash5 DN mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

KASH5-DN (CCDC155 B. tarus (bovine))

Use

Pgehme

Tags

mClover3

Promoter

T7

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMHE-GST-KASH5-DN

Plasmid

#171493

Purpose

T7 promotor drives in vitro transcription of GST-tagged bovine Kash5 DN mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

KASH5-DN (CCDC155 B. tarus (bovine))

Use

Pgehme

Tags

GST

Promoter

T7

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDG335

Plasmid

#100899

Purpose

SpCas9n (D10A Nickase mutant) with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

Depositor

Paul Thomas

Article

Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

Inserts

humanized CRISPR associated protein 9 Nickase
U6-gRNA scaffold 1
U6-gRNA scaffold 2

Use

CRISPR and Mouse Targeting

Tags

HA

Expression

Mammalian

Mutation

D10A mutant converts to Nickase

Promoter

CBh and U6

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pME V5-mTagBFP2 (JDW 830)

Plasmid

#224484

Purpose

Gateway compatible middle entry clone containing V5 tagged mTagBFP2 (Cytosolic blue fluorescent reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

V5-mTagBFP2

Use

Gateway cloning

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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ROSA26(MultiFPsΔPuro)

Plasmid

#140759

Purpose

Targeting vector to Gt(ROSA)26 locus for conditional expression of distinct FPs (Venus, mCherry, and mCerulean) responding to each site-specific-recombinase activity (Cre, Dre, and phiC31o).

Depositor

Hideyuki Okano

Article

Serizawa et al Development. 2019 Nov 4;146(21). pii: de

Inserts

puromycin-N-acetyltransferase
Venus
mCherry
mCerulean

Use

Mouse Targeting

Promoter

CAGGS

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mClover3-MAP4-MTBD

Plasmid

#171498

Purpose

T7 promotor drives in vitro transcription of mClover3-tagged mouse MAP4-Microtubule Binding Domain mRNA

Depositor

Melina Schuh

Article

So et al Science. 2019 Jun 28;364(6447). pii: 364

Insert

MAP4-MTBD (Map4 Mouse)

Use

Pgehme

Tags

mClover3

Promoter

T7

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti.tie1.H2B.eYFP.W

Plasmid

#51293

Purpose

Lentivector that encodes H2B-eYFP under the transcriptional control of the mouse TIE1 promoter

Depositor

Rusty Lansford

Article

Sato et al PLoS One. 2010 Sep 14;5(9):e12674. doi:

Insert

H2B-eYFP (H2BC21 Human)

Use

Lentiviral

Tags

H2B-eYFP

Promoter

mTie1

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.GFAP.iGluSnFr.WPRE.SV40

Plasmid

#98930

Purpose

AAV expression of glutamate sensor from GFAP promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

Has Service

AAV1, AAV5, and AAV9

Insert

iGluSnFr

Use

AAV

Expression

Mammalian

Promoter

GFAP

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pME EFS-rtTA/rtTA3 (JDW 1230)

Plasmid

#224525

Purpose

A Gateway compatible middle entry clone containing a rtTA-3rd generation transactivator

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EFS-rtTA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-GBF1(E794K)

Plasmid

#206416

Purpose

Mammalian expression of hGBF1(E794K)

Depositor

Kazuhisa Nakayama

Article

Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

Insert

GBF1 (GBF1 Human)

Tags

HA

Expression

Mammalian

Mutation

E794K

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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