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pSV2neo human IFN-gamma

Plasmid

#10954

Depositor

Howard Young

Publication

Young et al J Immunol. 1986 Jun 15. 136(12):4700-3.

Insert

interferon-gamma (IFNG Human)

Expression

Mammalian

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-Myc-Smad8

Plasmid

#11745

Depositor

Jeff Wrana

Publication

Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

Insert

SMAD8 (Smad9 Rat)

Tags

Myc

Expression

Mammalian

Available since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
pRK7-HA-S6K1-F5A

Plasmid

#8986

Depositor

John Blenis

Publication

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Tags

HA

Expression

Mammalian

Mutation

F5A

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
HA GST p85 S6K1 pRK5

Plasmid

#8466

Depositor

David Sabatini

Publication

Ali et al J Biol Chem 2005 Apr 4;.

Insert

p85 s6k1 (Rps6kb1 Rat)

Tags

ha/gst

Expression

Mammalian

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCMV-PE7-P2A-BSD

Plasmid

#224122

Purpose

Mammalian expression of SpCas9 PE7 prime editor with P2A-BSD marker

Depositor

Brittany Adamson

Publication

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-P2A-BSD

Tags

P2A-BSD, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Promoter

CMV

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET-MCN-10His-mStayGold (E138D)

Plasmid

#211361

Purpose

Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

Depositor

Mohan Balasubramanian

Publication

Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

Insert

mStayGold

Tags

10xHis-tag

Expression

Bacterial

Mutation

E138D

Promoter

T7

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-FLEX-CybSEP2-WPRE

Plasmid

#207661

Purpose

Monitoring neuropeptide release

Depositor

Sung Han

Publication

Kim et al bioRxiv. 2023 Jan 20:2023.01.19.524797.

Insert

Cytochrome b-561 (Cyb561 Mouse)

Use

AAV

Mutation

Histidine 86 and 159 to alanines, inserted 2 X SE…

Promoter

hSynapsin

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ8001 - pCMV-R8.91 (D64V)

Plasmid

#202687

Purpose

R8.91 plasmid with mutated integrase (D64V)

Depositor

Stanley Qi

Publication

Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

Insert

gag pol

Use

Lentiviral

Expression

Mammalian

Mutation

D64V

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pINDUCER21-puro_Gateway-3xFLAG

Plasmid

#172981

Purpose

Gateway Destination vector for doxycycline-inducible expression of ORF

Depositor

Michael Wehr

Publication

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Type

Empty backbone

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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V5-GFP-APEX2-NES_pLX208

Plasmid

#202018

Purpose

expresses GFP-APEX2 in the mammalian cytosol, lentiviral vector

Depositor

Alice Ting

Publication

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

V5-GFP-APEX2-NES

Use

Lentiviral

Tags

GFP and V5

Expression

Mammalian

Promoter

CMV

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAWP78-PVCpnf_pvc13-Ad5Knob

Plasmid

#198289

Purpose

Produces PVCs retargeted with knob domain from hAd5 virus

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-Ad5Knob

Expression

Bacterial

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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EB3N-VVDfast-mCl3-iLID

Plasmid

#190165

Purpose

Expresses EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

Depositor

Anna Akhmanova

Publication

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

EB3N-VVDfast-mClover3-iLID (MAPRE3 Human, Synthetic)

Tags

-VVDfast-mClover3-iLID

Expression

Mammalian

Mutation

1-189 aa from EB3

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAV2-hSyn-G-Flamp1

Plasmid

#188569

Purpose

green biosensor for cAMP in neurons

Depositor

Jun Chu

Publication

Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

Insert

G-Flamp1

Use

AAV

Tags

His tag

Promoter

hSyn

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-EFS-SaABE8e-bGH-U6-sgRNA-BsmBI

Plasmid

#189922

Purpose

AAV genome encoding SaABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

Depositor

David Liu

Publication

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

SaABE8e

Use

AAV

Mutation

SaCas9 D10A

Promoter

EFS

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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PB-PEmax

Plasmid

#187893

Purpose

All-in-one prime editor piggyBac transposon with PEmax

Depositor

Tobias Cantz

Publication

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

BPSV40 NLS and c-Myc NLS

Expression

Mammalian

Promoter

CAG

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_SFFv_Her2-BFP_RHL027

Plasmid

#164823

Purpose

Lentiviral vector for constitutive expression of HER2+TM(23-675)-BFP

Depositor

Wendell Lim

Publication

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Insert

Extracellular and transmembrane region of ERBB2 (ERBB2 Human)

Use

Lentiviral

Tags

tagBFP

Promoter

SFFV

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1_3XFlag

Plasmid

#182494

Purpose

Empty plasmid containing Flag tag for C-terminal tagging

Depositor

Dogus Altintas, Paolo Comoglio

Publication

Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

Insert

3xFlag with 5 Glycolic spacer

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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AKT-translocation-mStrawberry reporter

Plasmid

#158685

Purpose

This plasmid is a AKT pathway reporter. It has 1EF1a promoter driving the expression of truncated FoxO1 fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Publication

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-EML4-ALK variant 1

Plasmid

#183828

Purpose

Express EML4-ALK fusion variant 1 (E13;A20) in mammalian cells

Depositor

Aaron Hata

Publication

Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

Inserts

ALK Receptor Tyrosine Kinase (ALK Human)
EMAP Like 4 (EML4 Human)

Use

Lentiviral

Tags

The fusion protein of EML4 exon 1-13 and ALK exon…

Expression

Mammalian

Promoter

CMV

Available since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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CD9-mEGFP

Plasmid

#182864

Purpose

Localization: Tetraspanin/Membrane

Depositor

Salvatore Chiantia

Publication

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

human CD9 (CD9 Human)

Expression

Mammalian

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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