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pMSCV-FLAG-2xSTREP-CCND3(T283A)-Puro

Plasmid

#172618

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3(T283A) and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Thr283Ala

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pJT337 Lenti pEF-dCas9-3XFLAG-ZN471 KRAB-T2A-tagBFP-P2A-Blast

Plasmid

#187341

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-ZN471 KRAB-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF1a

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-KIF1B beta-600-1400

Plasmid

#79109

Purpose

Mammalian expression of GFP-KIF1B beta-600-1400

Depositor

Susanne Schlisio

Article

Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

Insert

GFP-KIF1B beta (600-1400 amino acids) (KIF1B Human)

Tags

GFP

Expression

Mammalian

Mutation

exon 25 missing (natural splice varient)

Promoter

CMV

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only
pDONR223_BIRC2_WT

Plasmid

#82188

Purpose

Gateway Donor vector containing BIRC2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BIRC2 (BIRC2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RNF2_WT

Plasmid

#82277

Purpose

Gateway Donor vector containing RNF2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RNF2 (RNF2 Human)

Use

Gateway entry vector

Mutation

S2T

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CBX3_WT

Plasmid

#82103

Purpose

Gateway Donor vector containing CBX3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CBX3 (CBX3 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAP3K2_WT

Plasmid

#82143

Purpose

Gateway Donor vector containing MAP3K2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP3K2 (MAP3K2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_JAZF1_WT

Plasmid

#82136

Purpose

Gateway Donor vector containing JAZF1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

JAZF1 (JAZF1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MET_WT

Plasmid

#82307

Purpose

Gateway Donor vector containing MET , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MET (MET Human)

Use

Gateway entry vector

Mutation

S705N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pXPR003-sgTP53-3

Plasmid

#118021

Purpose

Constitutive expression of sgRNA targeting human TP53

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

sgTP53-3 (TP53 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CCND3-Puro

Plasmid

#172619

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_CTNNB1_p.G34V

Plasmid

#81694

Purpose

Gateway Donor vector containing CTNNB1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry vector

Mutation

G34V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IER3_p.A155T

Plasmid

#81297

Purpose

Gateway Donor vector containing IER3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IER3 (IER3 Human)

Use

Gateway entry vector

Mutation

A127P, A155T

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_NFE2L2_WT

Plasmid

#81259

Purpose

Gateway Donor vector containing NFE2L2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NFE2L2 (NFE2L2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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BCL6-GFP G55A in Artichoke

Plasmid

#169935

Purpose

Overexpression of BCL6-GFP G55A mutant fusion

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Dec;588(7836):164-168. doi:

Insert

BCL6 G55A (BCL6 Human)

Use

Lentiviral

Tags

GFP

Mutation

160R>C

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_EIF1AX_p.G9R

Plasmid

#81282

Purpose

Gateway Donor vector containing EIF1AX , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

EIF1AX (EIF1AX Human)

Use

Gateway entry vector

Mutation

N4D, G9R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ATG5_WT

Plasmid

#82246

Purpose

Gateway Donor vector containing ATG5 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ATG5 (ATG5 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CCNG1_WT

Plasmid

#81767

Purpose

Gateway Donor vector containing CCNG1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CCNG1 (CCNG1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BCL6_WT

Plasmid

#81746

Purpose

Gateway Donor vector containing BCL6 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BCL6 (BCL6 Human)

Use

Gateway entry vector

Mutation

R160C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_NFE2L2_WT

Plasmid

#81770

Purpose

Gateway Donor vector containing NFE2L2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NFE2L2 (NFE2L2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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