We narrowed to 17,098 results for: IGH@

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pAGM51323

Plasmid

#153224

Purpose

Level 1 position 2 containing the Arabidopsis Rps5a promoter, Cas9 (Zcasi), and the nos terminator

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Arabidopsis Rps5a promoter, Cas9 (Zcasi), nos terminator

Use

Synthetic Biology

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pC3.1-caggs-Crimson-CAAX

Plasmid

#190041

Purpose

Red Fluorescent Protein for Long-Term Labeling of Fine Structures in Neurons

Depositor

Michael Lin

Article

Ning et al Front Cell Dev Biol. 2022 Jun 29;10:8934

Insert

Crimson

Tags

CAAX

Expression

Mammalian

Promoter

caggs

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-ABE-3'UTR-sgRNA-2xMS2

Plasmid

#132554

Purpose

Vector plasmid expressing ABEmax and sgRNA scaffold with MS2 replacing the Tetraloop and loop 2

Depositor

Baisong Lu

Article

Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

Inserts

ABEmax
sgRNA, with Tetraloop and loop 2 replaced by MS2 aptamer

Expression

Mammalian

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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sfTq2ox-PBP5

Plasmid

#117960

Purpose

Periplasmic expression of superfolder mTurquoise2ox (pNM077 pTHV037-DsbAss-LEGPAGL-sfTq2ox-EFGS-∆1-17-PBP5)

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

sfTq2ox-PBP5 (dacA )

Expression

Bacterial

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pZyxin-miRFP703

Plasmid

#79999

Purpose

Zyxin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

Zyxin (ZYX Human)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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xCas9(3.7)-ABE(7.10)

Plasmid

#108382

Purpose

Mammalian expression vector for xCas9(3.7)-ABE

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9(3.7)-ABE(7.10)

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp15-StefinA

Plasmid

#182405

Purpose

Bacterial expression of Vesicle Nucleating peptide15-stefinA fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp15-stefin (CSTA Human)

Tags

Vesicle Nucleating peptide 15 (VNp15)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pSL1777 (pEffector)

Plasmid

#160731

Purpose

Effector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins and crRNA

Expression

Bacterial

Promoter

J23119

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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Tol2-elavl3-GCaMP6s

Plasmid

#59531

Purpose

Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

Depositor

Misha Ahrens

Article

Vladimirov et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

Inserts

elavl3 promoter (elavl3 Zebrafish)
GCaMP6s

Available Since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA flag PPAR gamma E499Q

Plasmid

#8896

Depositor

Bruce Spiegelman

Article

Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

Insert

PPAR gamma (Pparg Mouse)

Tags

flag

Expression

Mammalian

Mutation

E499Q, a mutant in the AF2 domain. Binds PPARg l…

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pAWPSG-Po-nCas9-BE

Plasmid

#195740

Purpose

Express APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coli

Depositor

Seung-Goo Lee

Article

Jeong et al Chem Eng J, 2023

Insert

Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Po (phoenol-inducible promoter)

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-TS2126 RNA Ligase

Plasmid

#119941

Purpose

Express TS2126 RNA ligase in E. coli

Depositor

Takashi Ito

Article

Miura et al Nucleic Acids Res. 2019 May 22. pii: 549

Insert

TS2126 RNA ligase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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pNCS-Antares

Plasmid

#74279

Purpose

Constitutive expression of Antares in bacteria

Depositor

Michael Lin

Article

Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

Antares

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cyto-ExRai-AktAR2

Plasmid

#184048

Purpose

Cytosol-targeted ExRai-AktAR2.

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

Cyto-ExRai-AktAR2

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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CRISPR-StAR1 (Retro)

Plasmid

#222687

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

PGK without BbsI cutsite

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Twin-Strep_IRES-EmGFP

Plasmid

#113884

Purpose

Mammalian (inducible) protein expression, bicistronic expression of cytosolic EmGFP

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

pHR-CMV-TetO2

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCRISPRyl

Plasmid

#70007

Purpose

CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2015 Dec 29.

Inserts

Codon optimized Cas9 from S. pyogenes
sgRNA expression cassette

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

SCR1'-tRNA and UAS1B8-TEF(136)

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pMZ828

Plasmid

#155322

Purpose

Constitutive expression of PhyB(1-650)-VP16 in plants

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

PCaMV35S-PhyB(1-650)-VP16-NLS-Tnos

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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FAS promoter luciferase

Plasmid

#8890

Depositor

Bruce Spiegelman

Article

Kim et al J Clin Invest. 1998 Jan 1. 101(1):1-9.

Insert

Fatty Acid Synthetase Promoter (Fasn Rat, unknown - rat or mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pEVOL-NBK-1

Plasmid

#207639

Purpose

A plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

Pyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)

Expression

Bacterial

Mutation

The evolved synthetase NBK-1 contains mutations Y…

Promoter

araBAD

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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