We narrowed to 22,456 results for: his

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K20‐scFv‐pSMBP2

Plasmid

#159940

Purpose

To generate baculovirus for insect cell expression of anti beta-1 integrin scFv K20

Depositor

Sandra Schmid

Article

Lakoduk et al Traffic. 2020 Sep;21(9):590-602. doi: 10

Insert

anti beta-1 integrin scFv

Tags

6xHis tag, FLAG tag, MBP fusion, Sortase A recogn…

Expression

Insect

Promoter

Polyhedrin

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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19 pCOLI_A_SUMO

Plasmid

#160257

Purpose

N-term 6xHis SUMO 3C cleavable Amp resistant bacterial expression vector

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Tags

6xHis and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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Integrin-αv Headpiece M400GC

Plasmid

#159637

Purpose

Contains integrin alpha v residues 1–594 with the M400C mutation and Gly inserted prior to residue 400

Depositor

Timothy Springer

Article

Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

Insert

alpha v headpiece M400GC

Tags

6x His and Strep II

Expression

Mammalian

Mutation

M400C

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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40 pCOLI_K_MBP

Plasmid

#160183

Purpose

N-term MBP 3C cleavable Kan resistant bacterial expression vector

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Tags

6xHis and MBP

Expression

Bacterial

Promoter

T7

Available Since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pET-gSTEP1

Plasmid

#159376

Purpose

Expresses gSTEP1 in E. coli

Depositor

Roberto Chica

Article

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

gSTEP1

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pET11a-STEPtag

Plasmid

#159373

Purpose

Expresses STEPtag in E. coli

Depositor

Roberto Chica

Article

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

STEPtag

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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gNS2B47NS3 S135A

Plasmid

#155317

Purpose

DENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker S135A mutant

Depositor

Dahai Luo

Article

Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

Insert

DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor

Tags

His tag

Expression

Bacterial

Mutation

S135A

Promoter

M13

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMTTH+H1.4ΔCTD

Plasmid

#157797

Purpose

Expression of human linker histone H1.4 without c-terminal tail

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H1.4ΔCTD (H1-4 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pETduet+sfGFP-TetR-p300HAT

Plasmid

#157795

Purpose

Expression of sfGFP-TetR-p300HAT fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

sfGFP-TetR-p300 (EP300 Human)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean3-Cerulean3-FLARE-Cameleon

Plasmid

#123353

Purpose

Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Cerulean3-Cerulean3-FLARE-Cameleon

Tags

6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-cpVenus-FLARE-Cameleon

Plasmid

#123350

Purpose

Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-cpVenus-FLARE-Cameleon

Tags

6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL713

Plasmid

#140383

Purpose

Expresses CtcS protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-CtcS-T

Tags

6XHis

Expression

Bacterial

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-Cep131

Plasmid

#136875

Purpose

Mammalian expression of the centrosomal protein Cep131 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep131 (CEP131 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Centrin3

Plasmid

#136806

Purpose

Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrin3

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pET-tTALE-EmGFP-15b

Plasmid

#138280

Purpose

Express tTALE-EmGFP

Depositor

Kyubong Jo

Article

Shin et al Sci Rep. 2019 Nov 20;9(1):17197. doi: 10

Insert

7-mer tTALE-EmGFP

Use

TALEN

Tags

6x His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Cep78

Plasmid

#136827

Purpose

Mammalian expression of the centrosomal protein Cep78 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep78 (CEP78 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Cep72

Plasmid

#136819

Purpose

Mammalian expression of the centrosomal protein Cep72 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep72 (CEP72 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Cep57

Plasmid

#136817

Purpose

Mammalian expression of the centrosomal protein Cep57 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep57 (CEP57 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-STIL

Plasmid

#136795

Purpose

Mammalian expression of the centrosomal protein STIL N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-STIL (STIL Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac HT JS-Munc18c

Plasmid

#135555

Purpose

Express mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.

Depositor

Jingshi Shen

Article

Yu et al Methods Mol Biol. 2019;1860:237-249. doi

Insert

Munc18c

Tags

6xHis tag and TEV site

Expression

Insect

Promoter

polyhedrin promoter

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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