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pET30a-SARS-CoV2-ORF3b (S23Q)

Plasmid

#165088

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 ORF3b (S23Q)

Expression

Bacterial

Mutation

Stop codon to glutamine at 23rd amino acid

Available Since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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eSpCas9(1.1)_No_FLAG_ATP1A1_G2

Plasmid

#86610

Purpose

Expresses the ATP1A1 G2 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 exon 4. Px330-like plasmid

Depositor

Yannick Doyon

Article

Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

Insert

ATP1A1 G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)

Use

CRISPR; Co-selection via nhej using ouabain

Expression

Mammalian

Mutation

K848A, K1003A, & R1060A

Promoter

CBh

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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pLeo-A-a2-GEMS(JAK-STAT)

Plasmid

#209110

Purpose

Transient mammalian expression of the CC functionalized GEMS receptor A-a2-GEMS (JAK-STAT)

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

A-a2-GEMS(JAK-STAT)

Expression

Mammalian

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-SUMO-A'-l4-G'-IRES-EmGFP

Plasmid

#209127

Purpose

Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G' and EmGFP

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l4-G'-IRES-EmGFP

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-SUMO-A'-l4-G-IRES-EmGFP

Plasmid

#209126

Purpose

Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G and EmGFP

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l4-G-IRES-EmGFP

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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L-CRISPR-CTN (MLL-ctrl)

Plasmid

#69215

Purpose

Advanced lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; control, MLL-sgRNA + luciferase-sgRNA

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Inserts

SpCas9
Sp sgRNA scaffold
SFFV promoter
P2A-mNeonGreen

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pEMS1996

Plasmid

#49126

Purpose

AAV plasmid with UGT8 (Ple267) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple267-iCre

Use

AAV

Expression

Mammalian

Promoter

UGT8

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pUHD-AML1-ETO

Plasmid

#12429

Depositor

Dong-Er Zhang

Article

Rhoades et al Blood. 2000 Sep 15. 96(6):2108-15.

Insert

AML1-ETO (RUNX1, RUNX1T1 Human)

Expression

Mammalian

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pMT-Pav-GFP

Plasmid

#24286

Depositor

Ron Vale

Article

Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

Insert

Pavarotti (pav Fly)

Tags

GFP

Expression

Insect

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
ABEMax nSaCas9

Plasmid

#135364

Purpose

Expresion of SaCas9 nickase (D10A) with ABEMax at the N terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

ABEMax nSaCas9

Use

CRISPR

Expression

Mammalian

Mutation

(V82G)

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-GRAB_g5-HT2m

Plasmid

#208722

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT2m in neurons in the presence of Cre recombinase

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT2m

Use

AAV

Promoter

EF1a

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-BG505.DS-SOSIP.4mut-gp160

Plasmid

#123277

Purpose

Mammalian expression plasmid for Env from the BG505 HIV-1 isolate; DS-SOSIP.4mut variant

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BG505) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; SOSIP mutations; …

Promoter

CMV

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR221-CCL5.Pro

Plasmid

#79492

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) CCL5/RANTES promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

CCL5 Promoter (CCL5 Synthetic, Human)

Use

Multisite gateway entry clone

Promoter

CCL5 Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCEP4-BG505.DS-SOSIP.6mut-gp160

Plasmid

#123278

Purpose

Mammalian expression plasmid for Env from the BG505 HIV-1 isolate; DS-SOSIP.6mut variant

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BG505) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; SOSIP mutations; …

Promoter

CMV

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ7529 pHR (hU6-crLPT-EFS-PuroR-WPRE)

Plasmid

#214877

Purpose

Lentiviral vector encoding RfxCas13d targeting LPT guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crLPT-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pEMS2157

Plasmid

#70118

Purpose

Promoterless AAV plasmid with MCS to insert promoter for expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-MCS-EmGFP-WPRE

Use

AAV

Expression

Mammalian

Promoter

Promoterless

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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B1 Integrin

Plasmid

#186137

Purpose

For expression of recombinant beta1 integrin cytosolic domain in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

ITGB1 (ITGB1 Human)

Tags

MBP and untranslated His6 after stop codon

Expression

Bacterial

Promoter

Lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.JAG1-F1.GS.CAR-2G

Plasmid

#194461

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.

Depositor

Ana Sofia Coroadinha

Article

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-JAG1 CAR

Use

Lentiviral

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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139H2_LC

Plasmid

#206202

Purpose

For recombinant expression of the light chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells.

Depositor

Joost Snijder

Article

Peng et al Life Sci Alliance. 2024 Mar 20;7(6):e202

Insert

anti-MUC1 antibody 139H2 light chain (Igkc Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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GST-beta2(616-951)

Plasmid

#100743

Purpose

Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Tags

GST

Expression

Bacterial

Mutation

Long isoform

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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