We narrowed to 59,379 results for: %s

1. pGL-p21UTRm1

   Plasmid

   #20878

   Depositor

   Robert Blelloch

   Article

   Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

   Insert

   p21 3'UTR site 1 mutation (Cdkn1a Mouse)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Predicted miRNA binding site 1 (Position ~420) GC…

   Available Since

   Aug. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

2. TIA1 RRM-CFP-FRBx5

   Plasmid

   #103780

   Purpose

   expresses iPOLYMER component in mammalian cells

   Depositor

   Takanari Inoue

   Article

   Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

   Insert

   TIA1 RRM-CFP-FRBx5
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-21a_2xNLS_FnCpf1_MBP_protein_expression

   Plasmid

   #120801

   Purpose

   Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   2xNLS_FnCpf1
   

   Use

   CRISPR

   Tags

   MBP and NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV-puro-5A, 6A mMCL1-OM

   Plasmid

   #45818

   Depositor

   Joseph Opferman

   Article

   Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

   Insert

   5A, 6A mMCL-1-OM
   

   Expression

   Mammalian

   Mutation

   Changed Arginine to Alanine at 5th and 6th amino …

   Available Since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pDONR223-SLC7A11 sh926R

   Plasmid

   #118701

   Purpose

   entry clone for SLC7A11 insensitive to hairpin TRCN0000288926

   Depositor

   William Kaelin

   Article

   Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

   Insert

   SLC7A11 (SLC7A11 Human)
   

   Expression

   Mammalian

   Mutation

   wt , SLC7A11 sh926R

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 PARL-FLAG-CT S65A+T69A+S70A

   Plasmid

   #13616

   Depositor

   Luca Pellegrini

   Article

   Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

   Insert

   Presenilin-associated rhomboid-like (PARL, PARL Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Ser 65, Thr 69 and Ser 70 to Alanine

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA-FLAG-NEMO-C54/347A

   Plasmid

   #27268

   Depositor

   Thomas Gilmore

   Article

   Herscovitch et al Biochem Biophys Res Commun. 2008 Feb 29.

   Insert

   NEMO (IKBKG Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Cys54Ala Cys347Ala

   Available Since

   Feb. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA5FRT/TO-p97-RH-mycStrep

   Plasmid

   #31839

   Depositor

   Hemmo Meyer

   Article

   Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

   Insert

   p97
   

   Tags

   Strep and myc

   Expression

   Bacterial and Mammalian

   Mutation

   R155H

   Promoter

   CMV

   Available Since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pCMV-mGold-CAF1-C-10

   Plasmid

   #158003

   Purpose

   Visualization of nucleus in mammalian cells using mGold

   Depositor

   Francois St-Pierre

   Article

   Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

   Insert

   CAF1 (Chaf1a Mouse)
   

   Tags

   mGold

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV.U6.gLacZ.Cas9-T2A-GFP

    Plasmid

    #170361

    Purpose

    Negative control plasmid used for Crispr/cas9 based disruption.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

    Insert

    Cas9-T2A-GFP
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    June 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-EF1a-V5-Emerin

    Plasmid

    #120246

    Purpose

    Expresses V5-tagged Emerin in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Emerin (Emd Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    EF1a

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFUW-TetO-Txnip

    Plasmid

    #178437

    Purpose

    doxycycline-inducible expression of Mouse Txnip in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

    Insert

    Txnip (Txnip Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL-p21UTRm2

    Plasmid

    #20879

    Depositor

    Robert Blelloch

    Article

    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

    Insert

    p21 3'UTR site 2 mutation (Cdkn1a Mouse)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Predicted miRNA binding site 2 (Position ~1100) G…

    Available Since

    March 12, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pUPD2 PhiC31 attP:TMtb:P35S:attB (GB1494)

    Plasmid

    #160577

    Purpose

    35S promoter sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attP and attB)

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    PhiC31 PB (attP:TMtb:P35S:attB)
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. FnoCas12a-DR-cr in PBCSK-

    Plasmid

    #126642

    Purpose

    Cloning vector FnoCas12a DR-crRNA for expression in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    FnoCas12a Full Length Direct Repeat crRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 Promoter

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET19b-hMED9

    Plasmid

    #15365

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Paoletti et al Proc Natl Acad Sci U S A. 2006 Dec 12. 1

    Insert

    hMED9 (MED9 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    N/A

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

17. HEL binding scFv D44.1

    Plasmid

    #111718

    Purpose

    Yeast Surface Display of D44.1 as a reference starting point for design

    Depositor

    Sarel Fleishman

    Article

    Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

    Insert

    D44.1
    

    Tags

    cmyc tag

    Expression

    Yeast

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVL94 - pOp4:VND7

    Plasmid

    #116016

    Purpose

    destination vector for GreenGate cloning method, "Effector line", crossed with a "Driver line" the GOI, here VND7, will be expressed upon Dex-induction

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pOp4:B-dummy:VND7:VP16:tUBQ10::BastaR
    

    Expression

    Plant

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)-FluoSTEP-EKAR

    Plasmid

    #181858

    Purpose

    Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    FluoSTEP-EKAR
    

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. 49535-sgFMR1_E3B

    Plasmid

    #157783

    Purpose

    Expresses a sgRNA targeting the 3rd exon of human FMR1 gene

    Depositor

    Xinyu Zhao

    Article

    Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

    Insert

    FMR1 (FMR1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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