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pDONR223_STAG2_p.P572S

Plasmid

#81598

Purpose

Gateway Donor vector containing STAG2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAG2 (STAG2 Human)

Use

Gateway entry vector

Mutation

P572S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAG2_p.L997W

Plasmid

#81588

Purpose

Gateway Donor vector containing STAG2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAG2 (STAG2 Human)

Use

Gateway entry vector

Mutation

L997W

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAG2_p.R252Q

Plasmid

#81589

Purpose

Gateway Donor vector containing STAG2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAG2 (STAG2 Human)

Use

Gateway entry vector

Mutation

R252Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAG2_p.R541K

Plasmid

#81578

Purpose

Gateway Donor vector containing STAG2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAG2 (STAG2 Human)

Use

Gateway entry vector

Mutation

R541K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3IP1_WT

Plasmid

#81853

Purpose

Gateway Donor vector containing PIK3IP1 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3IP1 (PIK3IP1 Human)

Use

Gateway entry vector

Mutation

T251S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3IP1_p.G185E

Plasmid

#81339

Purpose

Gateway Donor vector containing PIK3IP1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3IP1 (PIK3IP1 Human)

Use

Gateway entry vector

Mutation

G185E; T251S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCEFL GNAS EE K32R

Plasmid

#249277

Purpose

Expression of Galphas K32R mutant (GNASK32R) EE-tagged (internal)

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

GNAS (GNAS Human)

Tags

Internal EE tag

Expression

Mammalian

Mutation

GNAS-K32R Internal EE-tag

Promoter

EF-1

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL GNAS EE N218I

Plasmid

#249279

Purpose

Expression of Galphas N218I mutant (GNASN218I) EE-tagged (internal)

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

GNAS (GNAS Human)

Tags

Internal EE tag

Expression

Mammalian

Mutation

GNAS-N218I Internal EE-tag

Promoter

EF-1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL GNAS EE P116L

Plasmid

#249278

Purpose

Expression of Galphas P116L mutant (GNASP116L) EE-tagged (internal)

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

GNAS (GNAS Human)

Tags

Internal EE tag

Expression

Mammalian

Mutation

GNAS-P116L Internal EE-tag

Promoter

EF-1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL GNAS EE WT

Plasmid

#249276

Purpose

Expression of Galphas (GNAS) wild type EE-tagged (internal)

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

GNAS (GNAS Human)

Tags

Internal EE tag

Expression

Mammalian

Mutation

Internal EE-tag

Promoter

EF-1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL GNAS EE T350I

Plasmid

#249280

Purpose

Expression of Galphas T350I mutant (GNAST350I) EE-tagged (internal)

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

GNAS (GNAS Human)

Tags

Internal EE tag

Expression

Mammalian

Mutation

GNAS-T350I Internal EE-tag

Promoter

EF-1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pSicoFlipped-CAG-GFP-WPRE-bGHpA

Plasmid

#231018

Purpose

For strong lentivirus-mediated expression of GFP

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

Enhanced GFP

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSicoFlipped-CAG-dTomato-WPRE-bGHpA

Plasmid

#231019

Purpose

For strong lentivirus-mediated expression of dTomato

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

dTomato

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX(cre)-OCaMP-WPRE

Plasmid

#229853

Purpose

pAAV vector for Cre-dependent OCaMP (orange calcium indicator) expression under the control of hSyn promoter

Depositor

Alexander Lohman

Article

Aggarwal et al bioRxiv 2025.07.28.667269; doi: https://

Insert

OCaMP

Use

AAV

Tags

6xHis

Expression

Mammalian

Available Since

Aug. 25, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Hamster

Plasmid

#238131

Purpose

This plasmid encodes the full-length hamster (Mesocricetus auratus) TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.

Depositor

Dirk Daelemans

Article

Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

Insert

TMEM106B

Use

Lentiviral

Tags

Blasticidin-P2A-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCBh-T7-SpCas9-P2A-mTagBFP2(RAS3575)

Plasmid

#223111

Purpose

pCBh Human expression plasmid for SpCas9 with with C-term BPNLS-P2A-mTagBFP2

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9-P2A-mTagBFP2

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-mTagBFP2

Expression

Mammalian

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-SpCas9-P2A-EGFP (MSP2582)

Plasmid

#223067

Purpose

pCAG human codon optimized wild-type SpCas9 expression plasmid with C-terminal NLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized SpCas9-P2A-EGFP

Use

CRISPR

Tags

NLS(SV40)-3xFLAG-P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-TBET-T2A-mCHERRY

Plasmid

#224296

Purpose

Expresses mouse TBET and mCherry under EF1a promoter

Depositor

Nikhil Joshi

Article

Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

Insert

TBET T2A mCherry (Tbx21 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pEF2-EFGR-hAxl

Plasmid

#225997

Purpose

Expresses a chimeric contruct having N-terminal human EGFR and C-terminal human Axl

Depositor

Raymond Birge, Sergei Kotenko

Article

Kimani et al Cell Commun Signal. 2016 Sep 6;14(1):19.

Insert

human EGFR N-Terminal human Axl C-Terminal Fusion (EGFR, AXL Human)

Expression

Mammalian

Promoter

EF1-a

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL.BMI1

Plasmid

#224711

Purpose

Expresses N-terminal hexahistidine-tagged human BMI1 (PCGF4) in insect cells.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

BMI1 (BMI1 Human)

Tags

6x histidine tag

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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