We narrowed to 24,468 results for: ina

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pQE60 R196A wgMDH

Plasmid

#204271

Purpose

Bacterial expression of R196A wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

R196A

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 R124A wgMDH

Plasmid

#204257

Purpose

Bacterial expression of R124A wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

R124A

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.1 HA pCAGGS

Plasmid

#206076

Purpose

expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

cacna1a (Cacna1a Rat)

Expression

Mammalian

Promoter

CMV/B-actin

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pKB222

Plasmid

#170012

Purpose

Encodes LacI-controlled expression of surface-displayed LL-37 and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
LL-37

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.SV40.Thbs1-HA.SV40(polyA)

Plasmid

#195552

Purpose

Expresses Thbs1 under control of short GFAP promoter

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Promoter

short GFAP

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2- mRuby-Rab35

Plasmid

#194338

Purpose

Expresses mRuby Rab35 in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Insert

Rab35 (RAB35 Human)

Tags

mRuby

Expression

Mammalian

Promoter

CMV IE94

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (I56E)

Plasmid

#192813

Purpose

Stable expression of 3xFLAH-NCOA4 (I56E mutant) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

I56E mutation in the N-terminal domain

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (L63R)

Plasmid

#192814

Purpose

Stable expression of 3xFLAH-NCOA4 (L63R mutant) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

L63R mutation in the N-terminal domain

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pQCXIP EGFP-ER [TM(SAC1)]

Plasmid

#186475

Purpose

Expression of the Endoplasmic Reticulum marker GFP-ER [TM(SAC1)] in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

SAC1 (SACM1L Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C-terminal transmembrane region of the protein: 5…

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hsChRmine-oScarlet-KV 2.1-WPRE

Plasmid

#183521

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

hsChRmine-oScarlet-Kv2.1

Use

AAV

Mutation

H33R

Promoter

CaMKIIa

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a DIO-hsChRmine-oScarlet-WPRE

Plasmid

#183526

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

hsChRmine-oScarlet

Use

AAV

Mutation

H33R

Promoter

Ef1a

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-DHFR F[1,2]-C (TRP1)

Plasmid

#177795

Purpose

Gateway destination vector to insert genes of interest having a C-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Tags

DHFR F[1,2]

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV AURKA-mTurq2

Plasmid

#157767

Purpose

Expression of AuroraA kinase fused to mTurquoise2 under CMV promoter

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLX304_zeo_mmFbxw7_R505C

Plasmid

#160104

Purpose

Expresses murine Fbxw7 alpha with the R505C loss-of-function mutation in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Fbxw7 alpha R505C (Fbxw7 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

R505C

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-QUASM1:mScarletNLS-SV40pA

Plasmid

#155124

Purpose

Tol2 vector containing QUASM1 reporter element upstream of mScarlet-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1:mScarletNLS

Use

Zebrafish expression

Promoter

QUASM1-E1b

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-pIgH-ESCargo(FTV,Crimson)

Plasmid

#140162

Purpose

Expresses a regulatable secretory cargo for mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Promoter

EF-1a

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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PKAcs-GGS4X-GFP

Plasmid

#108583

Purpose

Expresses PKAcs-GFP fusion in mammalian cells with flexible linker

Depositor

Robert Hughes

Article

Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

Insert

protein kinase A, catalytic subunit

Tags

GFP

Expression

Mammalian

Mutation

H87Q and W196R

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLacI-GFP-HEC1 WT

Plasmid

#114049

Purpose

Expression of exogenous LacI-GFP-HEC1 WT

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

GFP-HEC1 (NDC80 Human)

Expression

Mammalian

Mutation

WT HEC1 sequence

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PA-mCit-GW

Plasmid

#113448

Purpose

ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Gateway shuttle vector

Tags

ProteinA-mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCB32

Plasmid

#111911

Purpose

Expresses gRNA to target Nte1 locus and has HygR marker

Depositor

Yi Tang

Insert

gRNA cassette targeting Nte1 locus

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

SNR52p

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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