165,688 results

1. pTP1122

   Plasmid

   #104159

   Purpose

   Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)
   

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX_311-Cas9

   Plasmid

   #96924

   Purpose

   for Cas9 knockout, lentiviral expression of Cas9. Alternate plasmid name: pXR111

   Depositor

   William Hahn, John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

   Insert

   Cas9
   

   Use

   Lentiviral

   Tags

   3xFlag and V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA4/TO/cmycDrosha

   Plasmid

   #10828

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al Curr Biol. 2004 Dec 14. 14(23):2162-7.

   Insert

   Drosha (DROSHA Human)
   

   Tags

   myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAVS1-Pur-CAG-EGFP

   Plasmid

   #80945

   Purpose

   human AAVS1 site targeting donor plasmid for knocking-in EGFP expression cassette driven by CAG promoter

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

   Insert

   EGFP
   

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTARA

   Plasmid

   #31491

   Depositor

   Kathleen Matthews

   Article

   Wycuff et al Anal Biochem. 2000 Jan 1. 277(1):67-73.

   Insert

   RNA polymerase
   

   Expression

   Bacterial

   Mutation

   N823D

   Available Since

   June 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOpen-P45

   Plasmid

   #165570

   Purpose

   Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.

   Depositor

   FreeGenes Project, Drew Endy, Jennifer Molloy

   Insert

   P45
   

   Use

   Synthetic Biology

   Available Since

   Aug. 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pscAAV-CAG-GFP

   Plasmid

   #83279

   Purpose

   recombinant AAV vector packaging self-complementary GFP under the CAG promoter

   Depositor

   Mark Kay

   Article

   Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

   Insert

   enhanced green fluorescent protein
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHN924

   Plasmid

   #178885

   Purpose

   Expression of GST-His8-HRV3C

   Depositor

   Kazuhiro Abe

   Insert

   human rhinovirus 3C protease
   

   Tags

   GST, His8

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCTRE-CD4

   Plasmid

   #114010

   Purpose

   Dox-inducible Cas9 expression with a CD4 selection marker

   Depositor

   Charles Rudin

   Article

   Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA

    Plasmid

    #154867

    Purpose

    Flp-dependent hM4D(Gi) DREADD-mCherry

    Depositor

    Ulrik Gether

    Article

    Runegaard et al eNeuro. 2018 Jun 18;5(3). pii: eN-NWR-03

    Has Service

    AAV Retrograde and AAV8

    Insert

    hM4D(Gi)-mCherry
    

    Use

    AAV; Flp-frt

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    Human Synapsin-1

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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