We narrowed to 88,472 results for: MAL

1. pCAG_Xph15-mNeonGreen-CCR5TC

   Plasmid

   #135533

   Purpose

   Encodes a specific PSD-95 binder (Xph15) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph15
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

   Plasmid

   #139089

   Purpose

   Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   xCas9-NG
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV_Syn_Xph20_eGFP_CCR5TC

   Plasmid

   #187444

   Purpose

   Encodes a specific PSD-95 binder (Xph20) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph20
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. CIRTS-control

   Plasmid

   #213788

   Purpose

   CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and scramble control guide RNA is expressed under human Syn1 and U6 promoter, respectively.

   Depositor

   Timothy Bredy

   Article

   Liau et al Nat Commun. 2023 Nov 22;14(1):7616. doi:

   Insert

   CIRTS-Calm3-U6-control gRNA
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   Syn1, U6

   Available Since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBa-KIF5C 559-tdTomato-FKBP

   Plasmid

   #64211

   Purpose

   KIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tags

   Depositor

   Marvin Bentley, Gary Banker

   Article

   Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

   Insert

   Kif5c (Kif5c Rat)
   

   Tags

   FKBP and tdTomato

   Expression

   Mammalian

   Mutation

   motor domain aa1-559

   Promoter

   chicken Beta Actin

   Available Since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX317-CELF4

   Plasmid

   #115445

   Purpose

   Constitutive lentiviral expression

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   CELF4 (CELF4 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSP2443

   Plasmid

   #72252

   Purpose

   Human expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG_Xph15-SNAPf-CCR5TC

   Plasmid

   #135536

   Purpose

   Encodes a specific PSD-95 binder (Xph15) fused to SNAPf, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph15
   

   Tags

   SNAPf

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHes1(467 RBPj(-))-luc

   Plasmid

   #43805

   Depositor

   Ryoichiro Kageyama

   Article

   Nishimura et al Genomics. 1998 Apr 1;49(1):69-75.

   Insert

   Hes1 Promoter (-467 to +46) RBPj(-) (Hes1 Mouse)
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   Contains the murine Hes1 promoter (-467 to +46), …

   Promoter

   Hes1 promoter fragment (-467 to +46) (RBPj-)

   Available Since

   April 5, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pLKO.1-shCELF4-4404

    Plasmid

    #115465

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shCELF4-4404 (CELF4 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. CTCF-mAC donor (Neo)

    Plasmid

    #140645

    Purpose

    CTCF tagging with mAID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CTCF (CTCF Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. AICSDP-34: TNNI1-mEGFP

    Plasmid

    #114411

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TNNI1, via excisable Cas9-excisable hPGK-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

    Insert

    TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TNNI1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCI-neo-RL-ZEB1 200bmutx5

    Plasmid

    #35537

    Depositor

    Greg Goodall

    Article

    Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

    Insert

    3'UTR of ZEB1 (ZEB1 Budding Yeast, Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    5 mutations in miR200b seed regions of 3'UTR…

    Available Since

    May 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-sgAAVS1

    Plasmid

    #85802

    Purpose

    Cas9/CRISPR vector for cut at human AAVS1 gene intron1

    Depositor

    Roland Friedel

    Article

    Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

    Insert

    PPP1R12C (PPP1R12C Human)
    

    Expression

    Mammalian

    Available Since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. nAChR beta2 YFP

    Plasmid

    #15107

    Depositor

    Henry Lester

    Article

    Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

    Insert

    beta2-YFP-M (Chrnb2 Mouse)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

16. ACTB-(A)4x

    Plasmid

    #112058

    Purpose

    ACTB mRNA tagged with 4 copies of Riboglow (variant A)

    Depositor

    Amy Palmer

    Article

    Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

    Insert

    ACTB (ACTB Human)
    

    Tags

    Riboglow RNA tag

    Expression

    Mammalian

    Mutation

    4 copies of Riboglow tag after stop codon

    Promoter

    CMV

    Available Since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-SaPE2-P2A-BSD

    Plasmid

    #174818

    Purpose

    Mammalian expression of SaCas9 prime editor 2 with P2A-BSD marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    SaPE2-P2A-BSD
    

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEx1-pEF-H2B-mCherry-T2A-rTetR-EED

    Plasmid

    #78101

    Purpose

    Expresses rTetR-EED for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

    Depositor

    Michael Elowitz

    Article

    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

    Insert

    H2B-mCherry-T2A-rTetR-EED (EED Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    rTetR

    Expression

    Mammalian

    Promoter

    pEF alpha

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1 puro CXCR4 siRNA-1

    Plasmid

    #12271

    Depositor

    Bob Weinberg

    Article

    Orimo et al Cell. 2005 May 6. 121(3):335-48.

    Insert

    CXCR4 siRNA (CXCR4 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-UCOE-SFFV-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

    Plasmid

    #188765

    Purpose

    dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    dCas9-XTEN80-KRAB(Kox1)
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Promoter

    SFFV

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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