We narrowed to 19,373 results for: Kin

1. pCDB185

   Plasmid

   #80688

   Purpose

   for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEEH_04

   Depositor

   David Baker

   Article

   Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

   Insert

   gEEH_04
   

   Use

   Tags

   OsmY-10His-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDB285

   Plasmid

   #80687

   Purpose

   for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEEEH_04

   Depositor

   David Baker

   Article

   Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

   Insert

   gEEEH_04
   

   Use

   Tags

   OsmY-10His-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX-2T FRB Y2105A

   Plasmid

   #68726

   Purpose

   Point mutation Tyr 2105 → Ala in the FKBP12-rapamycin binding (FRB) domain of mTOR

   Depositor

   Jie Chen

   Article

   Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

   Insert

   FKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
   

   Use

   Tags

   GST-tagged

   Expression

   Bacterial

   Mutation

   FRB domain only aa2015-2114; Y2105A

   Promoter

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

4. 3604 pGEX4T-1 Bad S136A (104-141)

   Plasmid

   #8801

   Depositor

   Stanley Korsmeyer

   Article

   Harada et al Mol Cell 1999 Apr;3(4):413-22.

   Insert

   Bad (Bad Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Contains AA's 104-141; S136A

   Promoter

   Ptac

   Available since

   July 6, 2005

   Availability

   Academic Institutions and Nonprofits only

5. GAG-PKB-pcDNA3

   Plasmid

   #24651

   Depositor

   Jim Woodgett

   Article

   Jones et al J Exp Med. 2000 May 15. 191(10):1721-34.

   Insert

   PKB (AKT1 Human)
   

   Use

   Tags

   GAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 7, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pMXs-ERas CVLS-IP

   Plasmid

   #13828

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

   Insert

   ERas CVLS (Eras Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   CSVA (224-227) changed to CVLS (CAAX sequence of …

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

7. PCDNA3-N3FLAG-MYB-TAD(251-327)

   Plasmid

   #105611

   Purpose

   transient express MYB TAD fragment with 3*FLAG tag at N terminal in HEK 293T cells

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   TAD fragment of MYB (mouse), amino acids 251-327 (Myb Mouse)
   

   Use

   Tags

   3*FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AL08_pgRNA.DMD

   Plasmid

   #100293

   Purpose

   DMD intron 43-targeting gRNA expression plasmid

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

   Insert

   gRNA spacer
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 Polymerase III snRNA

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUASP YFP Rab32 WT

   Plasmid

   #37738

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab32 (Rab32 Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   Promoter

   GAL4

   Available since

   Nov. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

10. HA SEK AL pcDNA3.1 zeo (+) - SEK1 (A-L)

    Plasmid

    #24653

    Depositor

    Jim Woodgett

    Article

    Yan et al Nature. . 372(6508):798-800.

    Insert

    SEK1 (MAP2K4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Ser220Ala, Thr224Leu

    Promoter

    Available since

    June 21, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. p53-(1-393)-E285A-N268D

    Plasmid

    #35090

    Depositor

    Cheryl Arrowsmith

    Article

    Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 1-393 - E285A-N268D

    Promoter

    T7

    Available since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3-Barkor 1-91

    Plasmid

    #24395

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Barkor (ATG14 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-91.

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3-Barkor 1-180

    Plasmid

    #24396

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Barkor (ATG14 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Contains aa 1-180.

    Promoter

    Available since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pLXSN-H-Ras_V12-S35

    Plasmid

    #39517

    Depositor

    Julian Downward

    Article

    Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

    Insert

    HRAS V12 S35 (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    G12V and T35S

    Promoter

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

15. LentiV_Neo_SIK3_T142Q

    Plasmid

    #108110

    Purpose

    Lentiviral expression plasmid of human SIK3 cDNA (gatekeeper mutation) with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    SIK3 (SIK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    change Threonine 142 to Glutamine

    Promoter

    EFS promoter

    Available since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. M26I DJ-1 TEV site pET15b

    Plasmid

    #60685

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    M26I DJ-1 (PARK7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M26I and TEV site was inserted (see comments belo…

    Promoter

    T7

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-mCherry-LKB1(S431A)

    Plasmid

    #84641

    Purpose

    mCherry-tagged, LKB1(S431A); lacks C-terminal PKA phosphorylation site

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    mCherry-LBK1(S431A) (STK11 Human, Synthetic)
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Mutation

    PKA phosphorylation site Serine 428 mutated to Al…

    Promoter

    CMV

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSG5-FLAG-CaMKK alpha with CaMKKbeta RP domain

    Plasmid

    #33331

    Depositor

    Anthony Means

    Article

    Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

    Insert

    Calcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    CaMKKalpha RP domain (167-189) was replaced with …

    Promoter

    SV40

    Available since

    April 10, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pCR3-vsv-CenpB 1-166-Sgo1 N61I

    Plasmid

    #108498

    Purpose

    expression of vsv-CenpB 1-166-Sgo1 N61I (no PP2A binding)

    Depositor

    Susanne Lens

    Article

    Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

    Insert

    CenpB-Shugoshin 1 (SGO1 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    CenpB 1-166 fusion before Sgo1, no PP2A binding N…

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLXSN-H-Ras_V12-E38

    Plasmid

    #39520

    Depositor

    Julian Downward

    Article

    Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

    Insert

    HRAS V12 E38 (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    G12V and D38E

    Promoter

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only