We narrowed to 20,535 results for: Kin

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pAAV-CMVd1-NLS-GAL4-iLight2-VP16

Plasmid

#219550

Purpose

NLS-Gal4(1-65)-iLight2-VP16 construc expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

NLS-Gal4(1-65)-iLight2-VP16

Use

AAV

Expression

Mammalian

Mutation

The (SAGG)x4 linker between GAL4(1-65) and the PC…

Promoter

CMVd1

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-uTEV1-CB5

Plasmid

#171007

Purpose

HiLITR protease with CB5 C-terminal tmd targeting information (ER)

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-CB5(tmd)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

pTRE-tight (TetOn)

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcna1a-IRES-EGFP

Plasmid

#164965

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFP

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcna1a (kcna1a Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Promoter

actinb (actb2) promoter

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCR3-FLAG-Ubc13-C87A

Plasmid

#43939

Depositor

Bryant Darnay

Article

Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

Insert

ubiquitin-conjugating enzyme E2N (UBE2N Human)

Tags

2x FLAG

Expression

Mammalian

Mutation

C87A

Available Since

Nov. 23, 2017

Availability

Academic Institutions and Nonprofits only

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His-MBP-NRBF2

Plasmid

#99330

Purpose

Mammalian expression of NRBF2, which associates with the PI3KC3-C1 complex

Depositor

James Hurley

Article

Young et al Proc Natl Acad Sci U S A. 2016 Jul 19;11

Insert

Nuclear receptor-binding factor 2 (NRBF2 Human)

Tags

His-MBP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-BECN1

Plasmid

#99328

Purpose

Component for mammalian expression of recombinant PI3KC3-C1 complex

Depositor

James Hurley

Article

Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

Insert

Beclin 1 (BECN1 Human)

Tags

Strep-Strep-Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only
EYFP-Mito-7

Plasmid

#56596

Purpose

Localization: Mitochondria, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Mito (COX8A Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pBabe RFP1-Smad2 neo

Plasmid

#58491

Purpose

mammalian expression of mRFP1-tagged mouse Smad2

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

Smad2 (Smad2 Mouse)

Tags

mRFP1

Expression

Mammalian

Available Since

Aug. 19, 2014

Availability

Academic Institutions and Nonprofits only
pWZLblasti-HA-ERK5

Plasmid

#53175

Purpose

Expresses HA tagged human ERK5 from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK5 (MAPK7 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
BirA-Myc_N_MYC

Plasmid

#167715

Purpose

Expresses MYC in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

MYC (MYC Human)

Tags

Myc-BirA

Expression

Mammalian

Mutation

no mutations

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Golgi-ExRai-AktAR2

Plasmid

#184051

Purpose

Golgi-targeted ExRai-AktAR2.

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

Golgi-ExRai-AktAR2

Tags

N-terminal 35 amino acids from human endothelial …

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK4(glu)

Plasmid

#14813

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1995 Jul 21. 269(5222):403-7.

Insert

MKK4(glu) (MAP2K4 Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser-220Glu Thr-224Glu

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pScalps_puro_mIL-2Rα

Plasmid

#59917

Purpose

Expresses mouse interleukin-2 receptor alpha chain

Depositor

Silvia Monticelli

Article

Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

Insert

interleukin 2 receptor alpha (Il2ra Mouse)

Use

Lentiviral

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only
pGL3-OF (mutant TCF-4 sites)

Plasmid

#16559

Depositor

Bert Vogelstein

Article

Shih et al Cancer Res. 2000 Mar 15. 60(6):1671-6.

Insert

TCF-4 binding sites (TCF4 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

3 copies of mutant Tcf-4 binding sites

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf5(T31N)-HA

Plasmid

#79429

Purpose

Expresses C-terminally HA-tagged Arf5(T31N) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Hanai et al J Biochem. 2016 Feb;159(2):201-8. doi: 1

Insert

Arf5 (ARF5 Human)

Tags

HA

Expression

Mammalian

Mutation

changed Threonine 31 to Asparagine

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf5(Q71L)-HA

Plasmid

#79428

Purpose

Expresses C-terminally HA-tagged Arf5(Q71L) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Hanai et al J Biochem. 2016 Feb;159(2):201-8. doi: 1

Insert

Arf5 (ARF5 Human)

Tags

HA

Expression

Mammalian

Mutation

changed Glutamine 71 to Leucine

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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OA-986F (AAEL003877-dCas9-VPR)

Plasmid

#183993

Purpose

Expresses dCas9-VPR under the Ae. aegypti polyubiquitin promoter

Depositor

Omar Akbari

Article

Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

Insert

AAEL003877-dCAS9-VPR

Use

CRISPR

Tags

dCas9-VPR

Expression

Insect

Promoter

AAEL003887

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-VPS34

Plasmid

#99327

Purpose

Component for mammalian expression of recombinant PI3KC3-C1 complex

Depositor

James Hurley

Article

Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

Insert

VPS34 (PIK3C3 Human)

Tags

Strep-Strep-Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only
pLXSN-Axl

Plasmid

#65222

Purpose

Expression of Axl in mammalian cells

Depositor

Axel Ullrich

Article

Zhang et al Cancer Res. 2008 Mar 15;68(6):1905-15. d

Insert

AXL (AXL Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only
pSLIK TT 3xFLAG SMAD4 neo

Plasmid

#83273

Purpose

Lentiviral vector for inducible expression of SMAD4 with 3xFLAG N-terminal tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

SMAD4 (SMAD4 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only