We narrowed to 69,679 results for: TOR

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pSUMO His6 SUMO PYL1 (36-211)

Plasmid

#177843

Purpose

Bacterial Expression of PYL1

Depositor

Karsten Melcher

Article

Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

Insert

PYR1-like 1 (PYL1 Mustard Weed)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pHDR2_DAZL-T2A-mGreenLantern-PuroTK

Plasmid

#222917

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to DAZL.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mGreenLantern

Use

CRISPR

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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CABE-T2.19

Plasmid

#193265

Purpose

Expression of CABE-T2.19 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CABE-T2.19

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDisplay-GACh1.0

Plasmid

#106072

Purpose

express the genetically-encoded fluorescent ACh indicator GACh1.0 in mammalian cells

Depositor

Yulong Li

Article

Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

Insert

GPCR activation based ACh sensor (GACh) 1.0

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCI-Neo-gp78/JM20

Plasmid

#13303

Depositor

Allan Weissman

Article

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Insert

gp78 (AMFR Human)

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pLJC6-METAP1-3xFLAG

Plasmid

#163453

Purpose

lentiviral overexpression vector for human METAP1

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

METAP1 (METAP1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

See: comments

Promoter

Ubc

Available Since

March 4, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE puro Tusc5

Plasmid

#87952

Purpose

retroviral plasmid containing mouse Tusc5

Depositor

David James

Article

Fazakerley et al J Biol Chem. 2015 Sep 25;290(39):23528-4

Insert

Tumor Suppressor Candidate 5 (Tusc5 Mouse)

Use

Retroviral

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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Igfbp1-promoter/pGL3

Plasmid

#12146

Depositor

Domenico Accili

Article

Nakae et al EMBO J. 2000 Mar 1. 19(5):989-96.

Insert

IGFBP1 promoter (Igfbp1 Rat)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Contains the canonical insulin response sequence …

Available Since

Aug. 2, 2007

Availability

Academic Institutions and Nonprofits only
1037 pGL3 FasL promoter

Plasmid

#9028

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

Fas ligand promoter (FASLG Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pCDNA_B2AR-no-pep

Plasmid

#47439

Purpose

control FRET sensor for beta2-adrenergic receptor

Depositor

Sivaraj Sivaramakrishnan

Article

Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

Insert

beta2-adrenergic receptor (ADRB2 Human)

Tags

10 nm ER/K helix, HA, mCerulean, mCitrine, and no…

Expression

Mammalian

Promoter

pCMV

Available Since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only
pH2R-P2A-mCherry-N1

Plasmid

#84331

Purpose

Co-expresses (untagged) Histamine-2 receptor and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H2R-P2A-mCherry (HRH2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV_FKBP-V5-TWIST1

Plasmid

#215022

Purpose

AAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for human TWIST1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

AAV

Tags

FKBP-V5

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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Agarabi CHO Light Chain in pJ201

Plasmid

#111566

Purpose

Cloning vector containing CDS for the murine light chain of the model chimeric mouse-human IgG1 expressed by the Agarabi CHO cell line

Depositor

Cyrus Agarabi

Article

Velugula-Yellela et al Biotechnol Prog. 2018 Jan;34(1):262-270.

Insert

Murine light chain

Use

Synthetic Biology; Cloning vector

Promoter

None

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pLV_mU6-sgASCL1_hU6-sgRNA_hUbC-PuroR-P2A-GFP

Plasmid

#162337

Purpose

Lentiviral expression of sgASCL1 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

S. pyogenes sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6; mU6

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgGPT2_5

Plasmid

#163455

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting GPT2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 5 targeting GPT2 (GPT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCDSRaD-sdi1

Plasmid

#219566

Purpose

Mammalian expression of human sdi1 (p21 CDK inhibitor)

Depositor

Asao Noda, James R Smith

Article

Noda et al Exp Cell Res. 1994 Mar;211(1):90-8. doi:

Insert

sdi1 (CDKN1A Human)

Expression

Mammalian

Promoter

SRa

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(ICOS)

Plasmid

#177903

Purpose

Bacterial expression of his10-tagged human ICOS Intracellular Domain

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

ICOS (aa 162-199 only) (ICOS Human)

Tags

10xHis-Thrombin cut site-T7 tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAC147-pCR8-dCas9VP64

Plasmid

#48219

Purpose

dCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP64 activation domain

Use

CRISPR and Gateway Cloning

Tags

HA Tag and VP64

Mutation

D10A;H840A

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA_B2AR-q-pep

Plasmid

#47437

Purpose

FRET sensor for beta2-adrenergic receptor

Depositor

Sivaraj Sivaramakrishnan

Article

Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

Insert

beta2-adrenergic receptor (ADRB2 Human)

Tags

10 nm ER/K helix, HA, mCerulean, mCitrine, and q-…

Expression

Mammalian

Promoter

pCMV

Available Since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only
pCMV5 Flag-Smad1 (4SP/AP+AAVA)

Plasmid

#14955

Depositor

Joan Massague

Article

Kretzschmar et al Genes Dev. 1997 Apr 15. 11(8):984-95.

Insert

Smad1 (4SP/AP+AAVA) (SMAD1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Four Serine to Alanine mutations in the linker re…

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only