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pT7-EGFP-C1-HseIF4G1_615-654-L640AV646A_AD

Plasmid

#148519

Purpose

Mammalian Expression of HseIF4G1_615-654-L640AV646A

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G1_615-654-L640AV646A (EIF4G1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF4G_615-654-LIVVtoAAAA_AD

Plasmid

#148523

Purpose

Mammalian Expression of HseIF4G1_615-654-LIVVtoAAAA

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G1_615-654-LIVVtoAAAA (EIF4G1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF4G_615-654-Y619AL624AL625A_AD

Plasmid

#148524

Purpose

Mammalian Expression of HseIF4G1_615-654-Y619AL624AL625A

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G1_615-654-Y619AL624AL625A (EIF4G1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-724pUC19-tNGFR-P2A-EVER2

Plasmid

#186105

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

EVER2 (TMC8 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-721pUC19-tNGFR-P2A-RhoH

Plasmid

#186116

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

RHOH (RHOH Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-723pUC19-tNGFR-P2A-EVER1

Plasmid

#186088

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

EVER1 (TMC6 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-728pUC19-tNGFR-P2A-WIP

Plasmid

#186094

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

WIP (WIPF1 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-729pUC19-tNGFR-P2A-Moesin

Plasmid

#186095

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

MOESIN (MSN Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-455 tNGFR-P2A-WAS

Plasmid

#186082

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

WAS (WAS Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pmiRFP2-P2A-HO1-N1

Plasmid

#178966

Purpose

Expresses miRFP2 and HO1 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

miRFP2-P2A-HO1

Tags

no

Expression

Mammalian

Mutation

NO

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pnYC-HseIF4E_AF

Plasmid

#148669

Purpose

Bacterial Expression of HseIF4E

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4E (EIF4E Human)

Expression

Bacterial

Mutation

one silent mutation compared to the sequence of i…

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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SYKA-c022

Plasmid

#175490

Purpose

Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be biotinylated.

Depositor

Opher Gileadi

Article

Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

Insert

SYK (SYK Human)

Tags

AviTag and His6-TEV

Expression

Bacterial

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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330-BFP-hCYP3A4-enhancer-R-sgRNA

Plasmid

#176841

Purpose

Targeting human CYP3A4 proximal enhancer right boundary. sgRNA expressing cells could be FACS sorted by BFP expression.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

Human CYP3A4 proximal enhancer right boundary

Expression

Mammalian

Promoter

U6

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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330-cherry-hCYP3A4-enhancer-L-sgRNA

Plasmid

#176840

Purpose

Targeting human CYP3A4 proximal enhancer left boundary. sgRNA expressing cells could be FACS sorted by cherry expression.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

Human CYP3A4 proximal enhancer left boundary

Expression

Mammalian

Promoter

U6

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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XLone-Puro Cas13d-eGFP U6 RUNX1 g2

Plasmid

#155186

Purpose

PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and RUNX1 gRNA2

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Insert

Cas13d RUNX1 gRNA2

Use

Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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XLone-Puro Cas13d-eGFP U6 SOX17 g1

Plasmid

#155187

Purpose

PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and SOX17 gRNA1

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Insert

Cas13d SOX17 gRNA1

Use

Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11A

Plasmid

#159131

Purpose

Expresses BACH1 in mammalian cells

Depositor

Michele Pagano

Article

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Insert

BACH1 (BACH1 Human)

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Mutation

Y11A

Promoter

CMV

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-UbcH7(C86K)-10aa-linker-delta 1-29 and 117-160 Bactrocera parkin(C463A)

Plasmid

#128383

Purpose

expresses protein UbcH7 (C86K) fused to Bactrocera parkin (C463A) with residues 1-29 and 117-160 deleted

Depositor

Kalle Gehring

Article

Sauve et al Nat Struct Mol Biol. 2018 Jul;25(7):623-

Insert

UbcH7 (UBE2L3 Human)

Tags

Bactrocera dorsalis parkin with mutation C463A an…

Expression

Bacterial

Mutation

mutation C86K in UbcH7 gene

Promoter

lac promotor

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PHF21A__CAMK1D

Plasmid

#116831

Purpose

Lentiviral expression of PHF21A__CAMK1D fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PHF21A__CAMK1D

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PRKCE__CAMKMT

Plasmid

#116832

Purpose

Lentiviral expression of PRKCE__CAMKMT fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PRKCE__CAMKMT

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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