161,202 results

1. pLY005SB_pAAV-U6sg(BbsI)-EFS-SB100X

   Plasmid

   #192148

   Purpose

   T cell CRISPR AAV vector

   Depositor

   Sidi Chen

   Article

   Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV-mLin28A

   Plasmid

   #26357

   Depositor

   George Daley

   Article

   Viswanathan et al Nat Genet. 2009 Jul . 41(7):843-8.

   Insert

   lin-28 homolog A (Lin28a Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pRK5-FLAG-Sestrin2

   Plasmid

   #72595

   Purpose

   overexpression

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

   Insert

   SESN2 (SESN2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon optimized for mammalian expression, see dep…

   Promoter

   CMV

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pCfB6633

   Plasmid

   #106162

   Purpose

   EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6677 into Yarrowia lipolytica chromosomal location IntE_1, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Insert

   unknown
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDV05.0_pEF1a-iRFP720

   Plasmid

   #200407

   Purpose

   Expresses iRFP720 constitutively in mammalian cells

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   iRFP720
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR221-NR1H2

   Plasmid

   #79513

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H2 CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   LXR BETA (NR1H2 Human)
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   no Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHCMV-LCMV-WE

   Plasmid

   #15793

   Depositor

   Miguel Sena-Esteves

   Article

   Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

   Insert

   Lymphocytic choriomeningitis virus glycoprotein LCMVGP gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETDuet_huCAD_ICADL (TevS)

   Plasmid

   #100098

   Purpose

   dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences

   Depositor

   Jim Wells

   Article

   Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

   Insert

   DNA fragmentation factor subunit alpha (CAD, DFFA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   two caspase cleavage sites (DETD-117 and DAVD-224…

   Promoter

   T7

   Available since

   Jan. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS416-yZ3EV-Z3pr (RB3578)

   Plasmid

   #69099

   Purpose

   URA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr

   Depositor

   David Botstein

   Inserts

   yZ3EV cassette
   Z3 promoter
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

    Plasmid

    #139997

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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