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pPSU20-40-60-80

Plasmid

#173839

Purpose

Coexpresses 20, 40, 60 and 80 kD Penn State ladder proteins

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABPACCBPv3

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET22b-ELP[V]60

Plasmid

#219406

Purpose

(VPGVG)60

Depositor

Danielle Mai

Article

Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

Insert

ELP[V]60

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pNW538_pAAVS1-pAP1(2)-FlpO-ABI-P2A-PYL-FlpO-CAG-U1-frt-STOP-frt-U2-GFP-P2A-luc2-ZeoR

Plasmid

#192939

Purpose

FlpO-based digitizer circuit driven by AP1 promoter

Depositor

Wilson Wong

Article

Wong et al Nat Commun. 2021 Oct 22;12(1):6150. doi:

Inserts

AP1(2)/minTK promoter
FlpO-ABI
PYL-FlpO
CAG promoter
frt-STOP-frt
GFP
luciferase
pPGK
Zeocin resistance
BFP

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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PB-CRX-T2A-NeuroD1

Plasmid

#194607

Purpose

Piggybac Tet-ON plasmid for inducing hiPSCs into photoreceptor-like cells via CRX and NeuroD1 expression.

Depositor

Haruhisa Inoue

Article

Otsuka et al iScience. 2022 Mar 8;25(4):103987. doi:

Insert

CRX-T2A-NeuroD1

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP

Plasmid

#176015

Purpose

Mammalian expression of EGFP. Parton lab clone DFS

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pSP-GM1

Plasmid

#64739

Purpose

Yeast expression plasmid

Depositor

Jens Nielsen

Article

Chen et al FEMS Yeast Res. 2012 Aug;12(5):598-607.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_EGFP_Donor

Plasmid

#178088

Purpose

HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

EGFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human PGK1

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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c-Jun

Plasmid

#187902

Purpose

Expresses c-Jun in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

c-Jun (JUN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-RPS6KB2

Plasmid

#23533

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RPS6KB2 (RPS6KB2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

R482Stop

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pH7m34GW-35S:SNACS

Plasmid

#203576

Purpose

Plant SnRK2 kinase activity FRET sensor in plant expression vector

Depositor

Julian Schroeder

Article

Zhang et al eLife. 2020 May 28;9:e56351. doi: 10.755

Insert

SNACS

Use

Tags

StrepII

Expression

Plant

Mutation

none

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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