Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 160,082 results for: DES

Showing: 10721 - 10740 of 160082 results

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plx307 SHOC2 V5 R182E

Plasmid

#190580

Purpose

SHOC2 variant overexpression

Depositor

William Hahn

Article

Kwon et al Nature. 2022 Jul 13. pii: 10.1038/s41586

Insert

SHOC2 (SHOC2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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plx307 SHOC2 V5 M219V

Plasmid

#190581

Purpose

SHOC2 variant overexpression

Depositor

William Hahn

Article

Kwon et al Nature. 2022 Jul 13. pii: 10.1038/s41586

Insert

SHOC2 (SHOC2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9_mouse_Adar2_ccC

Plasmid

#158117

Purpose

deletion mADAR2

Depositor

Emanuela Felley-Bosco

Article

Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

Insert

Adar2 (Adarb1 Mouse)

Expression

Mammalian

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9_mouse_Adar2_ccA

Plasmid

#158118

Purpose

deletion mADAR2

Depositor

Emanuela Felley-Bosco

Article

Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

Insert

Adar2 (Adarb1 Mouse)

Expression

Mammalian

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9_mouse_Adar2_ccB

Plasmid

#158119

Purpose

deletion mADAR2

Depositor

Emanuela Felley-Bosco

Article

Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

Insert

Adar2 (Adarb1 Mouse)

Expression

Mammalian

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSPL3-hRPH3A_c.1853Awt

Plasmid

#190478

Purpose

minigene assay

Depositor

Alfredo Brusco

Article

Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

Insert

RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCPF101-pfQC-cyclase dead

Plasmid

#184878

Purpose

expresses soluble plasmodium falciparum QC protein with a histag, cyclase dead mutant

Depositor

Ferenc Scheeren

Article

Kolli et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

pfQC-cyclase dead

Tags

His tag

Expression

Bacterial

Mutation

F77A & Q79A

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-eSpCas9-2A-GFP-sgRNA-RDH11

Plasmid

#161924

Purpose

To generate RDH11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against RDH11 exon 2.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

sgRNA targeting RDH11 exon 2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-L452R-AVI

Plasmid

#176442

Purpose

Expresses SARS-CoV-2 RBD domain containing L452R mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-L452R (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

L452R

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-S477N-AVI

Plasmid

#176443

Purpose

Expresses SARS-CoV-2 RBD domain containing S477N mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-S477N (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

S477N

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-SD1-WA1-AVI

Plasmid

#176448

Purpose

Expresses SARS-CoV-2 RBD-SD1 domain from the WA1 strain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-SD1-WA1 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-N501Y-AVI

Plasmid

#176439

Purpose

Expresses SARS-CoV-2 RBD domain containing N501Y mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-N501Y (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

N501Y

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-NTD-W152C-AVI

Plasmid

#176431

Purpose

Expresses SARS-CoV-2 NTD domain containing W152C mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-NTD-W152C (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

W152C

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-NTD-E154K-AVI

Plasmid

#176433

Purpose

Expresses SARS-CoV-2 NTD domain containing E154K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-NTD-E154K (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

E154K

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-S2P-D614G-AVI

Plasmid

#176324

Purpose

Expresses SARS-CoV-2 S2P protein containing D614G mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-S2P-D614G (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

D614G

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_CoordinatorMutant#2

Plasmid

#173986

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motif #2 mutated

Use

Luciferase

Mutation

Coordinator motif mutation #2

Promoter

SV40 promoter

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-EC1.45-human_min1-opossum_min2

Plasmid

#173966

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Human minimal enhancer region 1 (min1) and opossum minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

See Depositor Comments Below

Promoter

SV40 promoter

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_7XCoordinatorMutant

Plasmid

#173994

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with all 7 Coordinator motifs mutated

Use

Luciferase

Mutation

7X Coordinator motif mutation #1-2-3-4-5-6-7

Promoter

SV40 promoter

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_min1-2_4XCoordinatorMutant

Plasmid

#173962

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 4 mutated Coordinator motifs

Use

Luciferase

Mutation

4 mutated Coordinator motifs

Promoter

SV40 promoter

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-humanEC1.45_p300peak1-2_CoordinatorMutant#1

Plasmid

#173985

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motif #1 mutated

Use

Luciferase

Mutation

Coordinator motif mutation #1

Promoter

SV40 promoter

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 10721 - 10740 of 160082 results

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