165,688 results

1. pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

   Plasmid

   #191797

   Purpose

   vector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized dCas13d
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   R239A, H244A, R858A, H863A

   Promoter

   CBh, SV40, hU6

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3zeta

   Plasmid

   #89347

   Purpose

   2nd generation lentiviral vector which expresses SNAP tagged TCRbeta and other TCR subunits (Jurkat alleles) separated via 2A sequences

   Depositor

   Ron Vale

   Article

   Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

   Insert

   Jurkat TCRalpha/beta alleles and CD3e and CD3z
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   May 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB_EF1a_HA-tNGFR_PuroR

   Plasmid

   #212652

   Purpose

   piggyBac vector to express HA-tNGFR with puromycin resistance

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   tNGFR
   

   Use

   Piggybac

   Tags

   HA tag

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFLAG_G495R MDA5

   Plasmid

   #216799

   Purpose

   Expresses a single point mutant of melanoma differentiation-associated protein 5

   Depositor

   Sun Hur

   Insert

   melanoma differentiation-associated protein 5 (IFIH1 Human)
   

   Expression

   Mammalian

   Mutation

   G495R

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pD3sE_SC12

   Plasmid

   #219720

   Purpose

   DV3 E dimer with fusion loop mutation and increased expression

   Depositor

   Brian Kuhlman

   Article

   Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

   Insert

   DV3 sE SC12
   

   Tags

   8xHis and human serum albumin (HSA)

   Expression

   Mammalian

   Mutation

   G106D, A257W, T260R, F277W, A278P, G29K, T33V, A3…

   Available Since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-6xHis-MBP-TEV site-TadA8r

   Plasmid

   #208318

   Purpose

   Overxpression MBP-TadA8r

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA8r
   

   Expression

   Bacterial

   Mutation

   mutations in TadA are described in the paper

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ecoli PancACe

   Plasmid

   #215712

   Purpose

   Expresses acetyl-CoA biosensor in Ecoli

   Depositor

   Katharine Diehl

   Article

   Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

   Insert

   PancACe
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA4/TO-CDC25A

   Plasmid

   #216511

   Purpose

   Expresses CDC25A in human cells under a tetracycline/doxycycline-inducible promoter

   Depositor

   John Diffley

   Article

   Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

   Insert

   CDC25A (CDC25A Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pP{ELAV-GeneSwitch}

   Plasmid

   #83957

   Purpose

   Expresses conditional RU486-dependent GAL4 protein under control of the elav promotor

   Depositor

   Haig Keshishian

   Article

   Osterwalder et al Proc Natl Acad Sci U S A. 2001 Oct 23;98

   Insert

   fusion protein consisting of the GAL4-DBD, human progesterone receptor-ligand-binding domain, and P65-AD (GAL4, RELA Budding Yeast, Human)
   

   Expression

   Insect

   Promoter

   elav

   Available Since

   Dec. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLJC5-LAMP1-RFP-2xFlag

    Plasmid

    #102931

    Purpose

    Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-Flag)

    Depositor

    David Sabatini

    Article

    Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

    Insert

    LAMP1
    

    Use

    Lentiviral

    Tags

    mRFP-2xFlag

    Promoter

    ubc

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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