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pAAV_GFAP_SypHer3s_cyto

Plasmid

#238969

Purpose

Mammalian astrocytes SypHer3s expression

Depositor

Dmitry Bilan

Article

Lanin et al J Biophotonics. 2020 Mar;13(3):e20190024

Insert

SypHer3s

Use

AAV

Expression

Mammalian

Promoter

GFAP

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV2ST

Plasmid

#239400

Purpose

An AAV entry vector with stabilized ITRs, permits packaging of desired inserts in cis.

Depositor

Fred Bunz

Insert

Green fluorescent protein

Use

AAV

Promoter

CMV

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pRSET A-pcStar

Plasmid

#226339

Purpose

Bacterial expression of RSFP pcStar; photoconvertible red fluorescent protein with high brightness, high photoconversion contrast, and early detectable fluorescence

Depositor

Pingyong Xu

Article

Zhang et al Nano Lett. 2020 Apr 8;20(4):2197-2208. d

Insert

pcStar

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAIP4_9C74

Plasmid

#234238

Purpose

Heterologous protein expression of variant of super folded GFP in Escherichia coli

Depositor

Christopher Bahl

Insert

9C74

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-mGold2t-Lysosome-N-20

Plasmid

#231778

Purpose

Visualization of lysosome in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-Lysosome-N-20 (Lamp1 Rat)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-HaloGFP-Na2.4

Plasmid

#228470

Purpose

Bacterial expression of green fluorescent sodium ion indicator HaloGFP-Na2.4

Depositor

Robert Campbell

Article

Takeuchi et al RSC Chem Biol. 2024 Dec 3. doi: 10.1039/

Insert

HaloGFP-Na2.4

Expression

Bacterial

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pNK116

Plasmid

#219742

Purpose

MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Nicotiana benthamiana

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

mutant of fungal hispidin-3-hydroxylase

Use

Synthetic Biology

Mutation

D37E, V181I, S323M, M385K

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCHA1.1-Rap2a-V5-Hygro

Plasmid

#195510

Purpose

lentiviral vector expressing RAP2A CDS with hygromycin selection

Depositor

Joanna Wysocka

Article

Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

Insert

Hygromycin

Use

Lentiviral

Expression

Mammalian

Promoter

hPGK

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLRD26

Plasmid

#208273

Purpose

Production of levopimaradiene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
LPS

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD10

Plasmid

#208262

Purpose

Production of labda-7,13(16),14-triene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

grTPS2
sclav_p0491

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209660

Purpose

Expression of iATPSnFR2, medium affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.HaloTag

Use

AAV

Mutation

A95K

Promoter

GFAP

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209656

Purpose

Expression of iATPSnFR2, medium affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.HaloTag

Use

AAV

Mutation

A95K

Promoter

CAG

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT254-litR

Plasmid

#202308

Purpose

litR repressor plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

litR

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT249-psrA

Plasmid

#202304

Purpose

psrA repressor plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

psrA

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRA1EGFP

Plasmid

#193791

Purpose

Expresses EGFP under the control of PR promoter, pUC origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

EGFP

Expression

Bacterial

Promoter

PR promoter

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_CaMKIIa_SERT_EYFP

Plasmid

#198737

Purpose

Serotonin transporter with EYFP fluorescent protein driven by CaMKIIa promoter on a AAV vector

Depositor

Lin Tian

Article

Vargas et al Science. 2023 Feb 17;379(6633):700-706.

Insert

Serotonin transporter and yellow fluorescent protein

Use

AAV

Expression

Mammalian

Promoter

CaMKIIa

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCytERM-EBFP2

Plasmid

#184939

Purpose

ER Visualization (OSER assay) Expresses EBFP2 in fusion with N-terminal part of p450

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

CytERM-EBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0100

Plasmid

#172789

Purpose

Level 0 module containing CT_Stop

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

Spacer module with stop codons.

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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p{3P3-dsRed::FRT-RC,attB}

Plasmid

#182177

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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p{ie1-DsRed::FRT-RC,attB}

Plasmid

#182191

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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