Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 128,399 results for: CIL

Clear keywords
Showing: 10761 - 10780 of 128399 results

  3. pX330-BsaIx2-DD-Cas9

    Plasmid
    #75380
    Purpose
    Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1
    Depositor
    Michele Calos
    Publication
    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DD-SpCas9
    Use
    CRISPR
    Tags
    FKBP12 L106P DD
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    75380-plasmid-map-sequence-id-192102

    Enlarge

  5. pCiGSΔΨ

    Plasmid
    #108312
    Purpose
    Foamy virus helper plasmid expressing Gag, driven by a CMV promoter. Ampicillin resistant.
    Depositor
    David Russell
    Publication
    Josephson et al Hum Gene Ther. 2004 Jan;15(1):87-92.
    Insert
    gag
    Expression
    Mammalian
    Mutation
    pCiGSΔΨ is a newer version of the pCiGS Gag expre…
    Promoter
    CMV
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    108312-plasmid-map-sequence-id-211440

    Enlarge

  6. pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaC

    Plasmid
    #22931
    Purpose
    Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PDZ binding motif (delta C)
    Depositor
    Alexandra Newton
    Publication
    Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PHLPP1-deltaC (PHLPP1 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    PHLPP-deltaC, deletion of PDZ binding domain--the…
    Promoter
    CMV
    Available since
    Feb. 4, 2010
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  7. LV-indLS2

    Plasmid
    #123068
    Purpose
    Lentiviral construct that delivers a tamoxifen inducible Cre. Upon recombination, luc2 and mStrawberry express. Used to image spontaneous tumorigenesis or subclonal cell populations in vivo.
    Depositor
    Kevin Brindle
    Publication
    Rodriguez et al J Pathol. 2014 Mar;232(4):449-57. doi: 1
    Inserts
    CreERT2 with intron
    Firefly luciferase
    mStrawberry
    Use
    Lentiviral and Luciferase; Fluorescence
    Expression
    Mammalian
    Mutation
    synthetic intron added as indicated
    Promoter
    CAGGS (after Cre mediated inversion) and CAGGS (f…
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    123068-plasmid-map-sequence-id-240523

    Enlarge

  8. pcDNA5FRT-EF-Pdgfralpha-EGFPN D842V

    Plasmid
    #66789
    Purpose
    Expression of constitutively active murine PDGFRalpha D842V tagged with GFP under the control of EF1a promoter
    Depositor
    Lotte Pedersen
    Publication
    Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PDGFRalpha (Pdgfra Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    D842V (constitutively active)
    Promoter
    EF1a
    Available since
    July 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  9. FUW TetO HA-AMPKa1 WT

    Plasmid
    #69824
    Purpose
    Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner
    Depositor
    Anne Brunet
    Publication
    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi
    Insert
    5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    minimal CMV promoter with tetracycline operator
    Available since
    April 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    69824-plasmid-map-sequence-id-139679

    Enlarge

  10. PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-ins

    Plasmid
    #78099
    Purpose
    Nuclear fluorescent reporter in mammalian cells, phiC31 attB for integration, can be silenced with TetR-CR or rTetR-CR (e.g. CR can be KRAB or DNMT3B).
    Depositor
    Michael Elowitz
    Publication
    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Histone H2B - Citrine (H2BC21 Human, Synthetic)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    5xTetO pEF alpha
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  11. DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct

    Plasmid
    #176836
    Purpose
    Targeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.
    Depositor
    Rudolf Jaenisch
    Publication
    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-VP64-2A-MS2-p65-HSF1-2A-GFP
    Use
    Unspecified
    Mutation
    See Depositor Comments Below
    Promoter
    Tet operator (tetracycline inducible expression)
    Available since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    176836-plasmid-map-sequence-id-346773

    Enlarge

  14. HA-CHMP4B

    Plasmid
    #139013
    Purpose
    Expresses HA-tagged human CHMP4B in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Insert
    human CHMP4B
    Tags
    HA
    Expression
    Mammalian
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    139013-plasmid-map-sequence-id-274403

    Enlarge

  15. pIA33

    Plasmid
    #120803
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp
    Depositor
    Craig Ellermeier, David S. Weiss
    Publication
    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-
    Inserts
    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Base-pairing region targets red fluorescent prote…
    Promoter
    Pgdh and Pxyl
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120803-plasmid-map-sequence-id-235316

    Enlarge

Showing: 10761 - 10780 of 128399 results