3,641 results
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Plasmid#170439PurposeExpress drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.DepositorAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only
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pAc5.1B-EGFP-DmTral_394-543-V5His6_D
Plasmid#146134PurposeInsect Expression of DmTral_394-543DepositorInsertDmTral_394-543 (tral Fly)
ExpressionInsectMutationtwo silent mutations (nucleotides A198C, A387G) a…Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmBelle_1-298_B
Plasmid#145913PurposeInsect Expression of DmBelle_1-298DepositorAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-Fluc-CG7785_A
Plasmid#145863PurposeInsect Expression of CG7785-3UTRDepositorInsertCG7785-3UTR (CG7785 Fly)
ExpressionInsectMutation317bp deletion in 3'UTR region (4172-4273bp)…Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-Fluc-nerfin_E
Plasmid#146190PurposeInsect Expression of nerfin-3UTRDepositorInsertnerfin-3UTR (nerfin-1 Fly)
ExpressionInsectAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmUPF2-siRNAres-V5His6_E
Plasmid#146166PurposeInsect Expression of DmUPF2-siRNAresDepositorInsertDmUPF2-siRNAres (Upf2 Fly)
ExpressionInsectMutation3 non silent mutations: L1022P, V1097L and N1213S…Available SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTR(EF)-tRNA(Q)
Plasmid#170517PurposePCR template vector for amplifying gRNAcore(EF)-tRNA(Q) fragment; used together with pAC-U63-tgRNA-nlsBFP or pAC-U63-tgRNA-Gal80DepositorInsertgRNAcore(EF)-tRNA(Q)
UsePcr template vector for amplifying grnacore(ef)-t…Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23KR42EF44A-V5His6_C
Plasmid#146071PurposeInsect Expression of DmTral-LSmR21EE23KR42EF44ADepositorInsertDmTral-LSmR21EE23KR42EF44A (tral Fly)
ExpressionInsectMutationR21E, E23K, R42E, F44A. Additionally, one silent …Available SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-R42EF44A_C
Plasmid#146061PurposeInsect Expression of DmTral-R42EF44ADepositorInsertDmTral-R42EF44A (tral Fly)
ExpressionInsectMutationR42E, F44A. Additionally, two silent mutations A1…Available SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-R21EE23KS13AI15A-V5His6_C
Plasmid#146049PurposeInsect Expression of DmTral-LSmR21EE23KS13AI15ADepositorInsertDmTral-LSmR21EE23KS13AI15A (tral Fly)
ExpressionInsectMutationS13A, I15A, R21E, E23K, one silent mutation A198C…Available SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-FDF-ADA_C
Plasmid#146039PurposeInsect Expression of DmTral-FDF-ADADepositorInsertDmTral-FDF-ADA (tral Fly)
ExpressionInsectMutationFDF to ADA (407-409) mutation. Additionally, thre…Available SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-5Box-7-Fluc-STOP-CG10011-SV40_D
Plasmid#146156PurposeInsect Expression of 5BoxB-7-Fluc-CG10011-3UTRDepositorInsert5BoxB-7-Fluc-CG10011-3UTR
ExpressionInsectAvailable SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMe31B_268-459_D
Plasmid#146119PurposeInsect Expression of DmMe31B_268-459DepositorInsertDmMe31B_268-459 (me31B Fly)
ExpressionInsectAvailable SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1-V5His6_D
Plasmid#146095PurposeInsect Expression of DmPABPC1DepositorInsertDmPABPC1 (pAbp Fly)
ExpressionInsectMutationseven silent mutations compared to the sequence g…Available SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmSmaug_763-999-V5His6_C
Plasmid#146013PurposeInsect Expression of DmSmaug_763-999DepositorInsertDmSmaug_763-999 (smg Fly)
ExpressionInsectMutation2 silent mutations and a deletion of the AA 961-9…Available SinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-R42E_B
Plasmid#145988PurposeInsect Expression of DmTral-R42EDepositorInsertDmTral-R42E (tral Fly)
ExpressionInsectMutationtwo silent mutations A198C, A387G and a mutation …Available SinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmSmaug_1-762-V5His6_C
Plasmid#146001PurposeInsect Expression of DmSmaug_1-762DepositorInsertDmSmaug_1-762 (smg Fly)
TagsV5-6xHisExpressionInsectMutationNP_523987.1, 7 silent mutations compared to the s…Available SinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmZuc_B
Plasmid#145949PurposeInsect Expression of DmZucDepositorInsertDmZuc (zuc Fly)
ExpressionInsectAvailable SinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmExu_B
Plasmid#145939PurposeInsect Expression of DmExuDepositorInsertDmExu (exu Fly)
ExpressionInsectAvailable SinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-F629V_A
Plasmid#145898PurposeInsect Expression of DmAGO1-F629VDepositorInsertDmAGO1-F629V (AGO1 Fly)
ExpressionInsectMutationV315A, F629V, H913R - variation with 2 non silent…Available SinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmLSm3_A
Plasmid#145888PurposeInsect Expression of DmLSm3DepositorInsertDmLSm3 (LSm3 Fly)
ExpressionInsectAvailable SinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmDCP2_209-791_A
Plasmid#145848PurposeInsect Expression of DmDCP2_209-791DepositorInsertDmDCP2_209-791 (DCP2 Fly)
ExpressionInsectAvailable SinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3. 3xFlag:PP4-19c[D85NH115N]
Plasmid#112247Purposeexpression of fly mutant PP4DepositorAvailable SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
AByH
Plasmid#164152PurposeIntegrates marker gene onto D. melanogaster Y chromosomeDepositorInsertY-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right
Available SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
AByD
Plasmid#164150PurposeIntegrates marker gene onto D. melanogaster Y chromosomeDepositorInsertY-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right
Available SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
AByC
Plasmid#164149PurposeIntegrates marker gene onto D. melanogaster Y chromosomeDepositorInsertY-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right
Available SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
AByB
Plasmid#164148PurposeIntegrates marker gene onto D. melanogaster Y chromosomeDepositorInsertY-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right
Available SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
AByA
Plasmid#163749PurposeIntegrates marker gene onto D. melanogaster Y chromosomeDepositorInsertY-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right
Available SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
1026L=U6-3-gRNA#1[Pol-γ35]
Plasmid#159674PurposePlasmid supports expression of gRNA targeting Pol-γ35 site #1, tagged with mini-White, and can be integrated with phC31.DepositorInsertU6-3-gRNA#1[Pol-γ35]
ExpressionInsectAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
1026M=U6-3-gRNA#2[Pol-γ35]
Plasmid#159675PurposePlasmid supports expression of gRNA targeting Pol-γ35 site #2, tagged with mini-White, and can be integrated with phC31.DepositorInsertU6-3-gRNA#2[Pol-γ35]
UseCRISPRExpressionInsectAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_vhhGFP4::T48-Baz::mCherry
Plasmid#163927PurposeExpression of GrabFP-A-extracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (extracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_vhhGFP4::Nrv1::tagBFP
Plasmid#163925PurposeExpression of GrabFP-B-extracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (extracellular) nanobody with Nrv1 protein and tagBFP fluorophor
UseCre/LoxTagstagBFPExpressionInsectAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
908I
Plasmid#160241PurposeVanO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-SV40-Ubiquitin-Renilla-attBDepositorInsertsVan Operator (2x)
hsp70 minimal promoter
p-element promoter
Firefly luciferase
Renilla luciferase
Ubiquitin promoter
TagsT2A GFPExpressionInsectAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
907F1
Plasmid#160233PurposeFlightN-VanR-VP16-3X-SV40-attBDepositorInsertsVanR
Flightin promoter
TagsVP16ExpressionInsectAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSC-CMV-HA-Ast-3-NP-1-IRES-mCherry
Plasmid#159631Purposeexpresses HA Allatostatin-3, Neurophysin-1, IRES mCherry under the CSC promoterDepositorInsertAst (AstA Fly)
UseLentiviralTagsHA, IRES, mCherry, and neurophysin-1ExpressionMammalianPromoterCMV promoterAvailable SinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1045B
Plasmid#125004PurposeExpresses gRNAs targeting hid, eve, and hedgehogDepositorAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1045D
Plasmid#125006PurposeExpresses gRNAs targeting hedgehog and winglessDepositorAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1045E
Plasmid#125007PurposeExpresses tRNA-flanked gRNAs targeting hid, eve, and hedgehogDepositorAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1045A
Plasmid#125003PurposeExpresses gRNAs targeting hid and eveDepositorAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-Hel25E MUT
Plasmid#110122PurposeExpresses Flag-tagged D. melanogaster Hel25E with 4 mutations that impact circRNA nuclear export activityDepositorInsertHel25E (Hel25E Fly)
TagsFlagExpressionInsectMutation4 mutations (KKLN motif changed to RSFS)PromoterMetallothionein Promoter (pMT)Available SinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1050I (notch)
Plasmid#132421Purposeexpress arrays of gRNA targeting Notch under dU6-3 promoterDepositorInsertnotch gRNA array
ExpressionInsectPromoterU6-3Available SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1050K (firefly luciferase)
Plasmid#132422Purposeexpress arrays of gRNA targeting Firefly Luciferase under dU6-3 promoterDepositorInsertfirefly Luciferase gRNA array
ExpressionInsectPromoterU6-3Available SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1050U (cinnabar)
Plasmid#132423Purposeexpress arrays of gRNA targeting Cinnabar under dU6-3 promoterDepositorInsertcinnabar gRNA array
ExpressionInsectPromoterU6-3Available SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1050V (wingless)
Plasmid#132424Purposeexpress arrays of gRNA targeting Wingless under dU6-3 promoterDepositorInsertwingless gRNA array
ExpressionInsectPromoterU6-3Available SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1050Z4 (yellow)
Plasmid#132425Purposeexpress arrays of gRNA targeting Yellow under dU6-3 promoterDepositorInsertyellow gRNA array
ExpressionInsectPromoterU6-3Available SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1052B (Ubiq-Fluc_Ubiq-Rluc)
Plasmid#132426PurposeExpress Firefly Luciferase-eGFP and Renilla Luciferase under Ubiquitin-63E promoterDepositorInsertsUbiq promoter
firefly luciferase
T2A-eGFP
Renilla Luciferase-SV40-P10
reversed ubiq promoter
ExpressionInsectAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1050L (UASt-CasRx)
Plasmid#132418PurposeExpress CasRx under UASt promoterDepositorInsertsCasRx
UASt+hsp70
ExpressionInsectPromoterUAStAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
Drosophila Nedd2-like caspase (DRONC)
Plasmid#157778PurposeE. coli expression vector pET23 with C-term 6xHis tagDepositorInsertDrosophila Nedd2-like caspase (DRONC) (Dronc Fly)
Tags6x HISExpressionBacterialPromoterT7Available SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
Opie2-PuroR[dsxM]
Plasmid#131616PurposeExpresses a functional PuroR only in males and dsRed in both genders.DepositorAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only