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Showing: 1061 - 1080 of 3688 results

  4. KK501: pMVP (L3-L2) FKBP DD + polyA

    Plasmid
    #121789
    Purpose
    pMVP L3-L2 entry plasmid, contains FKBP DD + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term FKBP degron domain (stabilized by SHLD1) to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    FKBP Degron Domain + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121789-plasmid-map-sequence-id-237290

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  14. KJ901: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS

    Plasmid
    #121835
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121835-plasmid-map-sequence-id-237389

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  17. IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1

    Plasmid
    #121824
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    Sa-dCas9/LSD1 (open)
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121824-plasmid-map-sequence-id-237376

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  18. Myopathy Loop Mutant Nuclear Myosin I YFP

    Plasmid
    #60243
    Purpose
    Expresses a myopathy loop mutant (6 mutations) of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding activity
    Depositor
    Primal de Lanerolle
    Article
    Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.
    Insert
    Nuclear Myosin 1 (Myo1c Mouse)
    Use
    Tags
    EYFP and NLS
    Expression
    Mammalian
    Mutation
    Deleted I339, I340, G343, E344, E345,L346
    Promoter
    CMV
    Available since
    Dec. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 1061 - 1080 of 3688 results