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TDP-43_CTD_A321V

Plasmid

#98671

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with A321V

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

A321V

Promoter

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSpCAS9 wt (BB)-2A-GFP targeting human TRIM21

Plasmid

#138295

Purpose

gRNA for CRISPR/Cas9 knockout of human TRIM21

Depositor

Gaudenz Danuser

Article

Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

Insert

gRNA TRIM21 (TRIM21 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-KPTN

Plasmid

#87041

Purpose

mammalian expression of KPTN

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

KPTN (KPTN Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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MSP469

Plasmid

#65771

Purpose

Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

D1135V, R1335Q, and T1337R mutations in Cas9

Promoter

CMV & T7

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant

Plasmid

#101209

Purpose

Bacterial expression plasmid for SpCas9-HF1 variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant N497A/R661A/Q695A/Q926A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

N497A, R661A, Q695A and Q926A

Promoter

T7

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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MSP680

Plasmid

#65772

Purpose

Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

D1135E, R1335Q, and T1337R mutations in Cas9

Promoter

CMV & T7

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_A321G

Plasmid

#98670

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with A321G

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

A321G

Promoter

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti_CamKIIa_mKate2

Plasmid

#96942

Purpose

To label mature excitatory iPSC-derived human neurons

Depositor

Oleksandr Shcheglovitov, Ricardo Dolmetsch

Article

Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

Insert

far-red fluorescent protein

Use

Lentiviral

Tags

Expression

Mutation

E87D (please see depositor comments below)

Promoter

CaMKIIa

Available since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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QUAS-jGCaMP7s-T2A-tdTomato

Plasmid

#200010

Purpose

QUAS reporter line expressing GCaMP7s calcium indicator and tdTomato, separated by T2A

Depositor

Carolyn McBride

Article

Zhao et al Nature. 2022 May;605(7911):706-712. doi:

Insert

3xP3-ECFP-jGCaMP7s-td-Tomato

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-OC-IRES-BSD

Plasmid

#53118

Purpose

To create stable cell clones with high-level expression of Cas9 and OCT1

Depositor

Wensheng Wei

Article

Zhou et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Inserts

OCT1 (POU2F1 Human)
Cas9

Use

Lentiviral

Tags

IRES-BSD, NLS, and P2A

Expression

Mammalian

Mutation

human codon-optimized

Promoter

CMV

Available since

May 27, 2014

Availability

Academic Institutions and Nonprofits only
pTOPO-MCL1

Plasmid

#21605

Depositor

Ulrich Maurer

Article

Maurer et al Mol Cell. 2006 Mar 17. 21(6):749-60.

Insert

MCL-1 (MCL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Insert ends with a STOP codon, no V5-His-tag.

Promoter

Available since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only
TDP-43_CTD_M337P

Plasmid

#98675

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with M337P

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

M337P

Promoter

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_A326P

Plasmid

#98672

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with A326P

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

A326P

Promoter

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-C12orf66

Plasmid

#87047

Purpose

mammalian expression of C12orf66

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

C12orf66 (C12orf66 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-ITFG2

Plasmid

#87044

Purpose

mammalian expression of ITFG2

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

ITFG2 (ITFG2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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LITE2.0 EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_hGHpA

Plasmid

#47457

Purpose

LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

NLS(alpha-imp)-CRY2PHR-NLS-VP64

Use

Tags

2A_GFP

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pRSV-CaMKII(1-290)

Plasmid

#45065

Purpose

Expression of constit active CaMKII

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

CaM Kinase II (Camk2a Rat)

Use

Tags

Expression

Mammalian

Mutation

truncated at amino acid 290

Promoter

RSV

Available since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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CMV10-3xFlag Skp2 delta-F

Plasmid

#81116

Purpose

mammalian expression of F-box deleted Skp2

Depositor

Sung Hee Baek

Article

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

SKP2 (SKP2 Human)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

deleted F box

Promoter

CMV

Available since

Sept. 24, 2016

Availability

Academic Institutions and Nonprofits only
pRSV-CaMKIV

Plasmid

#45062

Purpose

Expression of CaMKIV

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

Cam Kinase IV (Camk4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

*

Promoter

RSV

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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plc242-GFP-WDR24

Plasmid

#87050

Purpose

mammalian expression of WDR24

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

WDR24 (WDR24 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge