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Plasmid#229001PurposeE547-mutant of human USP7DepositorInsertUSP7 1-546 (USP7 Human)
UseTags6xHis cleavable with thrombinExpressionBacterialMutationE547 mutated to stop codonPromoterT7Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
A2C-∆N
Plasmid#217909PurposeExpresses bicone gas vesicles in E. coliDepositorInsertA2C-∆N
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDL25_Cas9-His Δcys E1207C
Plasmid#206286PurposeBacterial expression of Cas9-His Δcys E1027C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationC80S, C574S, E1207CPromoterT7Available sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL23_Cas9-His Δcys E945C
Plasmid#206284PurposeBacterial expression of Cas9-His Δcys E945C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationC80S, C574S, E945CPromoterT7Available sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL24_Cas9-His Δcys E1026C
Plasmid#206285PurposeBacterial expression of Cas9-His Δcys E1026C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationC80S, C574S, E1026CPromoterT7Available sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL22_Cas9-His Δcys E532C
Plasmid#206283PurposeBacterial expression of Cas9-His Δcys E532C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationC80S, E532C, C574SPromoterT7Available sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-AtPCO1(wt)
Plasmid#191798PurposeFull length wt AtPCO1 with Nt-His tagDepositorInsertAtPCO1
UseTags6xHis, Thrombin cleavage siteExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-AtPCO2(wt)
Plasmid#191799PurposeFull length wt AtPCO2 with Nt-His tagDepositorInsertAtPCO2
UseTags6xHis, Thrombin cleavage siteExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-AtPCO3(wt)
Plasmid#191800PurposeFull length wt AtPCO3 with Nt-His tagDepositorInsertAtPCO3
UseTags6xHis, Thrombin cleavage siteExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_redesigned_Caspase-4_ANR261ENP_D315H_M318K_T352K_enzymatic domain
Plasmid#183387PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertredesigned Caspase-4 (AA94-377) (CASP4 Human)
UseTagsHis-TEVExpressionBacterialMutationANR261ENP, D315H, M318K, T352KPromoterT7Available sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
FAK C-term
Plasmid#186147PurposeFor expresseion of recombinant FAK c-terminal domain in E. coliDepositorInsertPTK2 (PTK2 Mouse)
UseTagsHis6-sumoExpressionBacterialMutationPromoterT7Available sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_hTLN2-head-LBD
Plasmid#164837PurposeExpression of human talin2 head mutantDepositorInsertTLN2 (TLN2 Human)
UseTagsHis8ExpressionBacterialMutationLipid binding deficient mutationsPromoterAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pTH861-gst-optec P210T
Plasmid#89595PurposePlasmid for bacterial expression of a His-tagged P210T mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase P210T mutant, codon-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationchanged proline 210 to threoninePromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH860-gst-optec A180Q
Plasmid#89594PurposePlasmid for bacterial expression of a His-tagged A180Q mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase A180Q mutant, codon-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationchanged alanine 180 to glutaminePromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH858-gst-optec V156E
Plasmid#89592PurposePlasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase V156E mutant, codon-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationchanged valine 156 to glutamic acidPromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH859-gst-optec Y172M
Plasmid#89593PurposePlasmid for bacterial expression of a His-tagged Y172M mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationchanged tyrosine 172 to methioninePromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ppj184 (JBEI-13431)
Plasmid#89784Purposefor expression of N-His-3607DepositorInsert3607
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
JPUB_006818
Plasmid#87924PurposeE. coli expression of K. stuttgartiensis proteinDepositorInsertKuste3608
UseTags6xHis and MBPExpressionBacterialMutationPromoterT7Available sinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only