We narrowed to 17,384 results for: puro

1. pAG CMV C-HA Puro

   Plasmid

   #237880

   Purpose

   Empty mammalian expression plasmid for cloning C-terminally HA-tagged proteins

   Depositor

   Addgene Research Program, Meghan Rego

   Type

   Empty backbone

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 12, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pLD-puro-Cc-IFIT5_K197R-VA

   Plasmid

   #191223

   Purpose

   Lentiviral expression of human IFIT5_K197R in mammalian cells

   Depositor

   Mohan Babu

   Insert

   interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
   

   Use

   Lentiviral

   Tags

   VA tag

   Expression

   Mammalian

   Mutation

   changed Lysine 197 to Arginine (K197R)

   Promoter

   CMV

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLD-puro-Cc-IFIT5_K206R-VA

   Plasmid

   #191224

   Purpose

   Lentiviral expression of human IFIT5_K206R in mammalian cells

   Depositor

   Mohan Babu

   Insert

   interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
   

   Use

   Lentiviral

   Tags

   3xFlag-TEVx2-6xHis-Strep x2 tag and VA tag

   Expression

   Mammalian

   Mutation

   changed Lysine 206 to Arginine (K206R)

   Promoter

   CMV

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLD-puro-Cc-IFIT5_WT-VA

   Plasmid

   #191221

   Purpose

   Lentiviral expression of human IFIT5_WT in mammalian cells

   Depositor

   Mohan Babu

   Insert

   interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
   

   Use

   Lentiviral

   Tags

   VA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLD-puro-Cc-IFIT5_K160R-VA

   Plasmid

   #191222

   Purpose

   Lentiviral expression of human IFIT5_K160R in mammalian cells

   Depositor

   Mohan Babu

   Insert

   interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
   

   Use

   Lentiviral

   Tags

   VA tag

   Expression

   Mammalian

   Mutation

   changed Lysine 160 to Arginine (K160R)

   Promoter

   CMV

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti Puro HA-Ubiquitin-KO

   Plasmid

   #236292

   Purpose

   Lentiviral vector expressing HA-tagged ubiquitin KO mutant

   Depositor

   Addgene Research Program, Melina Fan

   Insert

   Ubiquitin KO (UBB Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Single ubiquitin coding sequence containing KO mu…

   Promoter

   CMV

   Available since

   April 15, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pLenti Puro HA-Ubiquitin-WT

   Plasmid

   #236289

   Purpose

   Lentiviral vector expressing HA-tagged wild type ubiquitin

   Depositor

   Addgene Research Program, Melina Fan

   Insert

   Ubiquitin (UBB Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Single ubiquitin coding sequence (full length UBB…

   Promoter

   CMV

   Available since

   April 10, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pdest dTomato Puro

   Plasmid

   #223054

   Purpose

   dTomato gene expression in mammalian cells

   Depositor

   Alejandro Gutierrez

   Article

   Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

   Insert

   dTomato
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTBL3342 PB-PEmax-puro

   Plasmid

   #226674

   Purpose

   PiggyBac cargo vector encoding PEmax and puromycin resistance.

   Depositor

   Chang Liu

   Article

   Loveless et al bioRxiv 2021.11.05.467507

   Insert

   PEmax
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti puro HA-STX3

    Plasmid

    #227590

    Purpose

    Lentiviral plasmid expressing human STX3 protein

    Depositor

    Addgene Research Program, Melina Fan

    Insert

    STX3 (STX3 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-SAM-Puro

    Plasmid

    #198713

    Purpose

    For expression of guide RNAs and the SAM transcriptional activator for CRISPRa in mammalian cells.

    Depositor

    Tyler Jacks

    Article

    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

    Inserts

    U6-MS2-gRNAscaffold
    EF1a-MPH-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and U6

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. qTAG-KO-Puro-TP53

    Plasmid

    #227319

    Purpose

    Donor template for 2A-Puro insertion into the N-terminus of the TP53 locus. For selectable p53 knock-out. To be co-transfected with sgRNA plasmid px330-TP53 (Addgene #227318)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TP53 Homology Arms flanking a 2A-Puro Cassette (TP53 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. CROPseq-Puro-2xMS2

    Plasmid

    #212627

    Purpose

    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    EF1a-PuroR-2xMS2-WPRE-hU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. CROPseq-Puro-12xMS2

    Plasmid

    #212628

    Purpose

    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    EF1a-PuroR-12xMS2-WPRE-hU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CROPseq-Puro-4xMS2

    Plasmid

    #212626

    Purpose

    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    EF1a-PuroR-4xMS2-WPRE-hU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCSIIpuro-HBEGF-mNeonGreen

    Plasmid

    #209912

    Purpose

    To monitor the expression of HB-EGF, the plasmid encodes a recombinant HB-EGF fused intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    HBEGF-mNeonGreen (HBEGF Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCSIIpuro-TGFa-mNeonGreen

    Plasmid

    #209913

    Purpose

    To monitor the expression of TGFα, the plasmid encodes a recombinant TGFα fused intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    TGFa-mNeonGreen (TGFA Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCSIIpuro-EREG-mNeonGreen

    Plasmid

    #209914

    Purpose

    To monitor the expression of Epiregulin, the plasmid encodes a recombinant Epiregulin fused intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    EREG-mNeonGreen (EREG Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPBpuro-NRG1-ScNeo

    Plasmid

    #209906

    Purpose

    To monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    NRG1-ScNeo (NRG1 Human)
    

    Use

    Tags

    mNeonGreen and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti puro HA-H2BC21

    Plasmid

    #227589

    Purpose

    Lentiviral plasmid expressing human H2BC21 protein

    Depositor

    Addgene Research Program, Melina Fan

    Insert

    H2BC21 (H2BC21 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge