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pcDNA-h-mmPtch1-B-HA

Plasmid

#120902

Purpose

Expresses Ptch1-B for function assays

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

ptch1 (Ptch1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 sgLacZ-1

Plasmid

#74968

Purpose

sgLacZ-1, puromycin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

LacZ

Use

CRISPR

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 sgLacZ-2

Plasmid

#74969

Purpose

sgLacZ-2, puromycin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

LacZ

Use

CRISPR

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAN1572

Plasmid

#127202

Purpose

Dual inducible expression of Venus-degronSwitchA and KeyA-BFP

Depositor

Hana El-Samad

Article

Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

Insert

pGal1-Venus-degronSwitch_A_t12-tSSA1-pZ3-key_A_e18-mTagBFP2-tENO1

Use

Synthetic Biology

Available Since

Aug. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)MBII-85-3'ss mut

Plasmid

#67644

Purpose

MBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 3' splice site was mutated to consensus sequences.

Depositor

Stefan Stamm

Article

Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

Insert

MBII85 snoRNA

Expression

Mammalian

Mutation

3' splice site muattion

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pCLX-MIFE-hM3Dq-PGK-mCherry

Plasmid

#114294

Purpose

Mifepristone-inducible expression of hM3Dq protein

Depositor

Patrick Salmon

Article

Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

Insert

hM3Dq

Use

Lentiviral

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-GFP-Ubl4A-C(90-157)

Plasmid

#86923

Purpose

To express Ubl4A (90-157 residues) tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Residues from 1-89 are deleted

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pLX301-HSV1-VP11/12-epitope

Plasmid

#253505

Purpose

Expression of HSV-1 VP11/12 ATDSLNNEY epitope fused to IL-2 signal sequence

Depositor

Mark Lee

Article

Kasbe et al Sci Adv. 2026 Feb 13;12(7):eaeb1732. doi

Insert

HSV-1 VP11/12 peptide

Use

Lentiviral

Available Since

April 21, 2026

Availability

Academic Institutions and Nonprofits only

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pLX301-HCoV-NL63/229E-ORF1ab-epitope

Plasmid

#253501

Purpose

Expression of HCoV-NL63/229E ORF1ab AIDAYPLSK epitope fused to IL-2 signal sequence

Depositor

Mark Lee

Article

Kasbe et al Sci Adv. 2026 Feb 13;12(7):eaeb1732. doi

Insert

HCoV-NL63/229E ORF1ab peptide

Use

Lentiviral

Available Since

April 21, 2026

Availability

Academic Institutions and Nonprofits only

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puDNA5_SSF_DOR_GLUT4_AEYA_APEX2

Plasmid

#250772

Purpose

Mammalian cell expression of the delta opioid receptor with the last 17 amino acids of GLUT4, in which both leucines in the "LEYL" sequence are mutated to alanine

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

DOR-GLUT4(AEYA)

Tags

APEX2 and Signal Sequence FLAG

Expression

Mammalian

Mutation

delta opioid receptor with last 17 amino acids of…

Promoter

UBC

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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