164,826 results

1. pEGFP-BLM

   Plasmid

   #110299

   Purpose

   Mammalian expression of a EGFP-tagged full length Bloom's syndrome helicase

   Depositor

   Chris Kok-Lung Chan

   Insert

   BLM (Bloom's Syndrome) helicase (BLM Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pILGFPB5A

   Plasmid

   #83556

   Purpose

   Used to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   GAL1 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-EF1a-GalT-IRES-Puromycin

   Plasmid

   #134862

   Purpose

   Expresses EGFP-tagged Golgi marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   beta 1,4-galactosyltransferase (B4GALT1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 83-398

   Promoter

   EF1a

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAVS1-Neo-CAG-M2rtTA-H2BGFP (donor AB)

   Plasmid

   #85798

   Purpose

   donorAB targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP expression system

   Depositor

   Roland Friedel

   Article

   Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

   Insert

   PPP1R12C (PPP1R12C Human)
   

   Use

   Human targeting

   Tags

   Expression

   Mutation

   homology arms for knock-in into first intron

   Promoter

   Available Since

   March 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. STARD5A

   Plasmid

   #42392

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   STARD5A (STARD5 Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   See Comments

   Promoter

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

6. plentiCMV-sct-pp65-b2m-HLA-A0201

   Plasmid

   #196511

   Purpose

   Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

   Depositor

   Howard Chang

   Article

   Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

   Insert

   single chain of a pp65 peptide, b2m and HLA A0201
   

   Use

   Lentiviral

   Tags

   NA

   Expression

   Mammalian

   Mutation

   Y84C

   Promoter

   CMV

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTol1-UAS:ChrimsonR-tdTomato

   Plasmid

   #124231

   Purpose

   Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChrimsonR fused to the red fluorescent protein tdTomato

   Depositor

   Isaac Bianco

   Article

   Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

   Insert

   ChrimsonR-tdTomato
   

   Use

   Zebrafish expression

   Tags

   tdTomato

   Expression

   Mutation

   Promoter

   14xUAS-E1b

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-hNox5

   Plasmid

   #69354

   Purpose

   expresses human Nox5 in mammalian cells

   Depositor

   Karl-Heinz Krause

   Article

   Banfi et al J Biol Chem. 2001 Oct 5;276(40):37594-60

   Insert

   hNox5 (NOX5 Human)
   

   Use

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-mScarlet-I-P2A-iCre (JDW 917)

   Plasmid

   #224516

   Purpose

   A Gateway-compatible middle entry clone containing mScarlet-I P2A-iCre (codon optimized)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mScarlet-I-P2A-iCre
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV 4xSTAR-mNeonGreen-NLS-blast

    Plasmid

    #136258

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 4 repeats
    

    Use

    Lentiviral

    Tags

    mNeonGreen-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-mNeptune2.5

    Plasmid

    #197197

    Purpose

    Expresses the protein of mNeptune2.5 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    mNeptune2.5
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOSIP-KT (KanR, P21)

    Plasmid

    #45987

    Depositor

    Drew Endy, Keith Shearwin

    Article

    St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-ULK2

    Plasmid

    #97075

    Purpose

    Expresses ULK2 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

    Insert

    ULK2 (ULK2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV_NLS-dSaCas9-NLS-VPR

    Plasmid

    #68495

    Purpose

    AAV vector containing nuclease null SaCas9 fused to VPR

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    dSaCas9
    

    Use

    AAV

    Tags

    VPR

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTol2_NBT:Cas9green; erbb2_gRNA171

    Plasmid

    #199337

    Purpose

    pDEL134; transgenic construct to express cell-specific Cas9 in neurons; ubiquitous erbb2 sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    NBT
    Cas9
    erbb2 sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLPCX mito Grx1-roGFP2

    Plasmid

    #64977

    Purpose

    Mammalian expression of mitochondrial Grx1-roGFP2 (retroviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Retroviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    mitochondrial targeting sequence

    Promoter

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFR_CrPV_xb

    Plasmid

    #11509

    Depositor

    Phil Sharp

    Article

    Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

    Insert

    Firefly luciferase, Cricket Paralysis Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. MARIO/ pcDNA3

    Plasmid

    #124838

    Purpose

    mammalian expression of MARIO, a genetically-encoded fluorescent Mg2+ sensor

    Depositor

    Takeharu Nagai

    Article

    Maeshima et al Curr Biol. 2018 Feb 5;28(3):444-451.e6.

    Insert

    MARIO
    

    Use

    Tags

    No tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-anti-PDL1(KN035) PAGER(TF)

    Plasmid

    #230003

    Purpose

    Expresses anti-PDL1 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    IL2SP-Aro6-KN035-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    V360A/R361A on KORD

    Promoter

    CMV

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR1A no ccDB (w48-1)

    Plasmid

    #17398

    Purpose

    Gateway entry 1A vector, no ccDB gene

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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