We narrowed to 17,264 results for: tes

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J135F_BattB_Vif(T27X4)-IRES-SpyTag-sfmCherry2(11)-PuroR

Plasmid

#200637

Purpose

Recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells

Depositor

Kenneth Matreyek

Publication

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

Vif

Expression

Mammalian

Mutation

T27stop

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pJS506

Plasmid

#200712

Purpose

Promoter region from ATPase PMA1 as Pro5U promoter module for heterologous expression of plasma membrane localized transporters in yeast

Depositor

Jens Boch

Publication

Fuhrmeister et al Bio Protoc. 2023 Aug 5;13(15):e4733. doi

Insert

PMA1 promoter (PMA1 Budding Yeast)

Use

Synthetic Biology; Modular cloning

Mutation

domesticated for MoClo cloning (removal of intern…

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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LV-PGK-PVR-P2A-Cre-EFS-mScarletSIIN

Plasmid

#172435

Purpose

Lentivirus, expresses Cre recombinase, mouse PVR (CD155), and mScarlet-SIINFEKL

Depositor

Tyler Jacks

Publication

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

Cre
PVR
mScarlet-SIINFEKL(OVA257-264)+Ova 323-339

Use

Lentiviral

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-Nedd1-mOrange2

Plasmid

#196860

Purpose

Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2

Depositor

Frank Bradke

Publication

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Nedd1-mOrange2 (Nedd1 Rat)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-Camk2d

Plasmid

#186820

Purpose

Fluorescent reporter for Camk2d expression

Depositor

Hung-Chun Chang

Publication

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Camk2d promoter (Camk2d Mouse)

Use

Luciferase

Promoter

Camk2d promoter

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-neo-μ4-(HA)3

Plasmid

#198180

Purpose

Expression of HA-tagged AP-4 μ4 in mammalian cells

Depositor

Juan Bonifacino

Publication

Mattera et al PLoS Pathog. 2014 May 15;10(5):e1004107.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AP-4 μ4

Tags

triple HA tag

Expression

Mammalian

Promoter

CMV

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MUC2 D1 with cleavable His tag

Plasmid

#181813

Purpose

Expresses human MUC2 D1 with cleavable His tag, residues 21-389

Depositor

Deborah Fass

Publication

Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

Insert

Muc2 D1 (MUC2 Human)

Tags

6xHistadine followed by TEV cleavage site

Expression

Mammalian

Promoter

CMV

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-NLS-MaSat ZF-18-mVenusN

Plasmid

#191392

Purpose

Zinc finger fused to N-terminal part of split mVenus

Depositor

Albert Jeltsch

Publication

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

Flag-NLS-MaSat ZF-18-mVenusN

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLL5.0 hCortKD-Flcortmut3YF EGFP

Plasmid

#187263

Purpose

Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutant

Depositor

Alpha Yap

Publication

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

Cortactin (CTTN Human)

Use

Lentiviral

Tags

EGFP

Mutation

3YF (Y412, Y470, Y486)

Promoter

U6, 5'LTR

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pETDuet1-LanCL2-C321A/C367A-C-Ter-His

Plasmid

#154188

Purpose

To express LanCL2-C321A/C367A in bacterial cells

Depositor

Wilfred van der Donk

Publication

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL2

Tags

Hexahistidine

Expression

Bacterial

Mutation

Cys321->Ala321; Cys367->Ala367

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pNL-CEF-IgM

Plasmid

#187013

Purpose

Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgM

Depositor

Hermann Eibel

Publication

Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

Insert

Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgM

Use

Lentiviral

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pNL-CEF-IgA1

Plasmid

#187009

Purpose

Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1

Depositor

Hermann Eibel

Publication

Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

Insert

Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1

Use

Lentiviral

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pET15b MOSPD2 [282-490]

Plasmid

#186469

Purpose

Expression of human MOSPD2 (MSP domain from residue 282 to 490), fused to a 6HIS tag, in bacteria

Depositor

Catherine Tomasetto

Publication

Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Tags

6HIS

Expression

Bacterial

Mutation

MSP domain [282-490]

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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His10-B1 Integrin

Plasmid

#186139

Purpose

For expression of recombinant his10-beta1 integrin cytosolic domain in bacteria

Depositor

Michael Rosen

Publication

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

ITGB1 (ITGB1 Human)

Tags

MBP + uncleavable His10

Expression

Bacterial

Promoter

Lac

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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Crimson/P2A-NLS/HA-HumBeta (pSLIK2)

Plasmid

#113857

Purpose

For Doxycycline-inducible expression of Humanized Beta synaptase gene, with red fluorescent protein gene E2-Crimson-P2A-Humanized Beta synaptase gene

Depositor

Richard S Myers

Publication

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

Humanized Beta

Use

Lentiviral

Tags

P2A/Crimson

Mutation

"humanized" Beta gene

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pGEMHE-TNKS-2

Plasmid

#154149

Purpose

For making TNKS-2 RNA

Depositor

Carmen Williams

Publication

Gambini et al Dev Cell. 2020 Jun 8;53(5):545-560.e7. d

Insert

Tankyrase 2 (TNKS2 Human)

Use

In vitro transcription

Tags

FLAG

Promoter

T7

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSIN-Skil-gRNA2

Plasmid

#180371

Purpose

targeting mouse Skil/SnoN gene

Depositor

Dan Littman

Publication

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Skil targeting gRNA (Skil Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

human U6

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pSIN-Arkadia-gRNA4

Plasmid

#180365

Purpose

targeting mouse Arkadia gene

Depositor

Dan Littman

Publication

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Arkadia targeting gRNA (Rnf111 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

human U6

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pSIN-Arkadia-gRNA3

Plasmid

#180364

Purpose

targeting mouse Arkadia gene

Depositor

Dan Littman

Publication

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Arkadia targeting gRNA (Rnf111 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

human U6

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pST_MAHS_RAMVA_81-236-FLAG (pBS0297)

Plasmid

#185328

Purpose

For the mammalian expression of the tardigrade protein MAHS_RAMVA_81-236-FLAG. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MAHS_RAMVA_81-236-FLAG

Tags

FLAG

Expression

Mammalian

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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