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pAAV-EF1a-NCre-MBP5-WPRE

Plasmid

#193918

Purpose

Expresses one of the components of Cre-DOR_N5C4 (RFP-dependent Cre)

Depositor

Tatsushi Onaka

Article

Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

Insert

NCre-MBP5

Use

AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-puro HA-Atg14*dCC

Plasmid

#38266

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

autophagy related genes 14 (ATG14 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

changed A618T, A621T, C624A and T627C for siRNA r…

Promoter

LTR

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pX459-HypaCas9-mR2-RHOA_sgRNA

Plasmid

#183877

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and RHOA sgRNA for N-terminal tagging of RhoA in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

RHOA sgRNA spacer (RHOA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pF708-pET28a-Hs-delta38-METTL20-NHis wt

Plasmid

#85109

Purpose

expression of recombinant human mature (delta38) METTL20 wt with N-terminal 6xHis-tag

Depositor

Pal Falnes

Article

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human mature (delta38) METTL20 (METTL20 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-E2F3 3'UTR

Plasmid

#29470

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

E2F3 3'UTR (E2F3 Human)

Expression

Mammalian

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-hGRM1-RFP

Plasmid

#22920

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

human receptor metabotropic 1 promoter driving DsRedExpress (GRM1 Human)

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-mA93-RFP

Plasmid

#22918

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

mouse riken gene A930038C07Rik promoter driving DsRedExpress (A930038C07Rik Mouse)

Use

AAV

Expression

Mammalian

Available Since

Aug. 26, 2010

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-CHEK1 3'UTR

Plasmid

#29478

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

CHEK1 3'UTR (CHEK1 Human)

Expression

Mammalian

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pCI-neo-myc-LC3(deltaC22, G120A)

Plasmid

#45449

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

myc

Expression

Mammalian

Mutation

Encodes amino acids 1-120, Glycine at position 12…

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pLgw EcoDam-V5-SRF

Plasmid

#98602

Purpose

Mammalian DamID lentiviral vector forSRF with Dam-V5 using Gateway cloning

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

SRF (Srf Mouse)

Use

Lentiviral; Damid

Tags

Dam (DNA adenine methyltransferase) and V5

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pTE4566

Plasmid

#88904

Purpose

Expresses MbCpf1 crRNA and inactive/dead, humanized MbCpf1 nuclease

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Mb crRNA
hMbCpf1(D986A)

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV and human U6

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pRV-RB

Plasmid

#106374

Purpose

creating stable line with RFP and BSD resistance

Depositor

Tong-Chuan He

Article

Liao et al Gene Ther. 2017 Jun;24(6):333-341. doi:

Insert

mRFP and blasticidin resistance gene

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

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PA-RL-BAD

Plasmid

#78859

Purpose

DULIP positive control (bait) for assay establishment

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Insert

BCL2 associated agonist of cell death (BAD Human)

Use

Expression vector

Tags

Protein A, Renilla luciferase

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-hSOX2

Plasmid

#193351

Purpose

Retroviral vector expressing human SOX2

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

SOX2 (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-EE-UdgX-NG-nCas9-RBMX

Plasmid

#163571

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-EE-UdgX-NG-nCas9-RBMX

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

Available Since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag-RARalpha (T181A,S445A,S461A)

Plasmid

#35556

Depositor

Jonathan Kurie, Harish Srinivas

Article

Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

Insert

RARalpha (RARA Human)

Tags

Flag and His

Expression

Mammalian

Mutation

T181A, S445A, S461A

Available Since

April 5, 2012

Availability

Academic Institutions and Nonprofits only
pRK5 beta4 1355T

Plasmid

#16038

Depositor

Filippo Giancotti

Article

Nikolopoulos et al Mol Cell Biol. 2005 Jul . 25(14):6090-10

Insert

beta4 (ITGB4 Human)

Expression

Mammalian

Mutation

1355T: amino acids 1-1355

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pXPR_BRD003-sgQKI-2201

Plasmid

#115449

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgQKI-2201 (QKI Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgQKI-2204

Plasmid

#115450

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgQKI-2204 (QKI Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1puro-shKIBRA-A

Plasmid

#40888

Depositor

Paul Reynolds

Article

Moleirinho et al Oncogene. 2012 May 21. doi: 10.1038/onc.

Insert

shKIBRA-A (WWC1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only