We narrowed to 15,679 results for: Nes

Showing: 10801 - 10820 of 15679 results

Results per page:

Sort results by:

pcDNA 3.1 myc-Rab10-mito

Plasmid

#208367

Purpose

Mammalian expression of myc-tagged human Rab10 with C-terminal mito signal

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

myc-Rab10-mito (RAB10 Human)

Expression

Mammalian

Mutation

wildtype

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b+His SUMO Rab10(Q68L)

Plasmid

#207342

Purpose

Bacterial expression of His SUMO Rab10(Q68L)

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

Rab10 (RAB10 Human)

Tags

His-SUMO

Expression

Bacterial

Mutation

Q68L

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PET-HypaR-SpCas9-NLS-6xHis

Plasmid

#207387

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HypaR-SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R661A, N692A, M694A, Q695A, H698A

Promoter

T7

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
lenti-Cas9-sgHPRT1

Plasmid

#196713

Purpose

CRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TG

Depositor

Bernhard Schmierer

Article

Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

Insert

Cas9-T2A-BSD-U6-sgHPRT1

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Promoter

EF1a/hU6

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GLE_sgRNA

Plasmid

#186658

Purpose

GLE sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Gle sgRNA Plasmid (CG14749 Fly)

Expression

Insect

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Shu_sgRNA

Plasmid

#186656

Purpose

shu sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Shu sgRNA Plasmid (shu Fly)

Expression

Insect

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
puc19_N_tag

Plasmid

#186650

Purpose

Empty donor plasmid.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

N-terminal 3xFlag-3xHA Tag

Tags

3xFlag-3xHA

Expression

Insect

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJCC_103 SpCas9 T1337C

Plasmid

#179525

Purpose

For bacterial expression of SpCas9 T1337C (for DNA cross-linking) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

Insert

SpCas9 T1337C

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed threonine 1337 to cysteine

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-HA-mIFITM1-C49,50,83A

Plasmid

#58420

Purpose

Expresses murine IFITM1-C49,50,83A with an N-terminal HA tag in mammalian cells. (mutated at the 3 evolutionarily conserved cysteines)

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Cysteines 49, 50, and 83 to Alanine

Promoter

CMV IE

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pAAV_3x_U6_Ctnnb1_CAG_nlsTagBFP2

Plasmid

#247933

Purpose

Targeting Ctnnb1 gene in vivo by adeno-associated viral vector-mediated delivery

Depositor

Benoit Vanhollebeke

Article

Panji et al Commun Biol. 2026 Feb 25;9(1):479. doi:

Insert

Ctnnb1 sgRNAs (Ctnnb1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

nls-TagBFP2

Expression

Mammalian

Promoter

U6

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_3x_U6_Ikbkg_CAG_nlsTagBFP2

Plasmid

#247934

Purpose

Targeting Ikbkg gene in vivo by adeno-associated viral vector-mediated delivery

Depositor

Benoit Vanhollebeke

Article

Panji et al Commun Biol. 2026 Feb 25;9(1):479. doi:

Insert

Ikbkg sgRNAs (Ikbkg Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

nls-TagBFP2

Expression

Mammalian

Promoter

U6

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Renilla-FLAG-firefly-K529E

Plasmid

#255007

Purpose

Used to test the rate of amino acid misincorporation in mammalian cells

Depositor

Joshua Mendell

Insert

Renilla-FLAG-firefly-K529E

Use

Luciferase

Tags

3xFlag

Expression

Mammalian

Mutation

firefly-K529E

Promoter

CMV

Available Since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
puDNA5_SSF_DOR_MRS_2Ala_APEX2

Plasmid

#250763

Purpose

Mammalian cell expression of the delta opioid receptor with the last 17 amino acids of the mu opioid receptor, in which the leucines in the LENL sequence have been mutated to alanines

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

DOR-MRS(2Ala)

Tags

APEX2 and Signal Sequence FLAG

Expression

Mammalian

Mutation

delta opioid receptor with last 17 amino acids of…

Promoter

UBC

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-dCas9-3XmCherry-CRY2

Plasmid

#249580

Purpose

Lentiviral vector for stable expression of dCas9-3XmCherry-CRY2hiclu fusion protein in mammalian cells

Depositor

Tom Misteli

Article

Stortz et al J Cell Sci. 2026 Jan 19:jcs.264574. doi:

Inserts

CRY2hiclu
Sp dCas9

Use

Lentiviral

Tags

3XmCherry

Expression

Mammalian

Mutation

Catalytically dead mutant Cas9

Promoter

CMV

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST14-His6-TEV-SpyCatcher003-cys

Plasmid

#249543

Purpose

Expresses SpyCatcher003 with a N-terminal 6x-His tag and a C-terminal cysteine in E.coli under the T7 promoter

Depositor

Kai Zinn

Article

Anaya et al bioRxiv 2025.07.09.663943

Insert

SpyCatcher003-cys

Tags

6x His and Cysteine

Expression

Bacterial

Promoter

T7

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-dCas9-mCherry-CRY2

Plasmid

#249579

Purpose

Lentiviral vector for stable expression of dCas9-mCherry-CRY2hiclu fusion protein in mammalian cells

Depositor

Tom Misteli

Article

Stortz et al J Cell Sci. 2026 Jan 19:jcs.264574. doi:

Inserts

CRY2hiclu
Sp dCas9

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Catalytically dead mutant Cas9

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pHS484_10xHis_MBP_TEV_dSpRY

Plasmid

#244832

Purpose

Bacterial expression of catalytically inactive SpRY Cas9 (dSpRY) for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

dSpRY Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHS24_10xHis_MBP_TEV_SpRY-Cas9

Plasmid

#244827

Purpose

Bacterial expression of SpRY Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpRY Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRRL-SFFV_V5-CHIC2-C6S_Tomato

Plasmid

#238128

Purpose

Expresses V5-tagged CHIC2 (with C6S mutation) with Tomato selectable marker.

Depositor

Arlene Sharpe

Article

LaFleur et al Nat Immunol. 2025 Sep;26(9):1476-1487. d

Insert

V5-tagged CHIC2 with C6S mutation

Tags

V5

Expression

Mammalian

Mutation

Changed cysteines from amino acids 102-109 to ser…

Promoter

SFFV

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-TO-HA-Δ37 PPM1H-mito

Plasmid

#208375

Purpose

Lentiviral expression of human Δ37-PPM1H with C-terminal mito signal

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

HA-delta37 PPM1H-mito (PPM1H Human)

Use

Lentiviral

Expression

Mammalian

Mutation

delta37

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge