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pMYH9-D1424H-FLAG

Plasmid

#183513

Purpose

Gateway expression vector encoding D1424H mutant MYH9 cDNA, with N-terminal Flag tag. For mammalian expression.

Depositor

Emanuele Panza

Article

Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

Insert

MYH9 (MYH9 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Aspartic acid 1424 to Histidine

Promoter

CMV

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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NES-EGFP-PH-BTKx2

Plasmid

#183652

Purpose

PtdIns(3,4,5)P3 biosensor

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

BTK (BTK Human, Frog)

Tags

X. laevis map2k1.L(32-44)-EGFP

Expression

Mammalian

Mutation

Amino acids 2-174 and 2-170

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-GluN2A [N327A/38R]

Plasmid

#177532

Purpose

Mammalian Expression Plasmid of anti-GluN2A/NR2A glutamate receptor (Rat). Derived from hybridoma N327A/38.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GluN2A/NR2A glutamate receptor (Rattus norvegicus) recombinant mouse monoclonal antibody (Grin2a Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-ADAM22 (cytoplasmic) [N46/30R]

Plasmid

#177555

Purpose

Mammalian Expression Plasmid of anti-ADAM22 (cytoplasmic) (Mouse). Derived from hybridoma N46/30.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-ADAM22 (cytoplasmic) (Mus musculus) recombinant mouse monoclonal antibody (Adam22 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, Beta3 [N87/25R]

Plasmid

#177573

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Beta3 (Mouse). Derived from hybridoma N87/25.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GABA(A)R, Beta3 (Mus musculus) recombinant mouse monoclonal antibody (Gabrb3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-TrpM7 [N74/25R]

Plasmid

#177569

Purpose

Mammalian Expression Plasmid of anti-TrpM7 (Mouse). Derived from hybridoma N74/25.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpM7 (Mus musculus) recombinant mouse monoclonal antibody (Trpm7 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-SK2 K+ channel [K78/29R]

Plasmid

#177448

Purpose

Mammalian Expression Plasmid of anti-SK2 K+ channel (Rat). Derived from hybridoma K78/29.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-SK2 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnn2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-neo-PP1-Alpha-H248K

Plasmid

#171268

Purpose

Mammalian expression of 3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant that loses enzymatic activity

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant (PPP1CA Human)

Tags

Flag

Expression

Mammalian

Mutation

PPP1CA-His248Lys (H248K)

Promoter

CMV

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pJEP321-pAAV-FullU6TO-SaCas9gRNAi(SapI)-CMV-TetR-P2A-GFP-KASH-WPRE-shortPA Empty Cassette

Plasmid

#113698

Purpose

Dox-inducible U6 SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pEB1-moxCerulean3

Plasmid

#103975

Purpose

Plasmid encoding moxCerulean3

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

moxCerulean3

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, Y66W, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-MQ1(C141S)-EGFP

Plasmid

#89635

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR

Tags

3*FLAG, 6*His, and T2A-EGFP

Expression

Mammalian

Mutation

C141S, TGC-->TCC

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-MQ1(S317A)-EGFP

Plasmid

#89636

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR

Tags

3*FLAG and 6*His

Expression

Mammalian

Mutation

S317A, AGC-->GCC

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRFAM7ADelta2bp

Plasmid

#62515

Purpose

mammalian expression of human duplicated Alpha7 with 2 bp deletion (CHRFAM7A-∆2bp)

Depositor

Sherry Leonard, Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7ADelta2bp (CHRFAM7A Human)

Expression

Mammalian

Mutation

partially duplication of CHRNA7 with 2-bp deletio…

Promoter

CMV

Available Since

March 20, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCDNA3.1 AT1AR-TSTS/4A

Plasmid

#45634

Depositor

Robert Lefkowitz

Article

Wei et al J Biol Chem. 2004 Nov 12;279(46):48255-6

Insert

AT1AR-TSTS/A (Agtr1a Rat)

Tags

HA

Expression

Mammalian

Mutation

Thr332, Ser335, Thr336, and Ser338 all changed to…

Promoter

CMV

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA-HA-hTRAF3 333-C

Plasmid

#44035

Depositor

Shao-Cong Sun

Article

Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

Insert

TNF receptor-associated factor 3 (TRAF3 Human)

Tags

1xHA

Expression

Mammalian

Mutation

Contains amino acids 334-568; S393G and K492N

Available Since

April 4, 2013

Availability

Academic Institutions and Nonprofits only
Myc-CK2beta 6KR (pAB1016)

Plasmid

#37361

Depositor

David Litchfield

Article

French et al J Biol Chem. 2007 Oct 5;282(40):29667-77

Insert

CK2beta 6KR (CSNK2B Human)

Tags

Myc

Expression

Mammalian

Mutation

K33R, K139R, K177R, K191R, K208R, K212R

Promoter

CMV-F

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA4-His-Max-A-PQBP1-Y65C

Plasmid

#25803

Depositor

Marius Sudol

Article

Tapia et al J Biol Chem. 2010 Jun 18;285(25):19391-4

Insert

PQBP1 with Y65C mutation (PQBP1 Human)

Tags

His and Xpress

Expression

Mammalian

Mutation

Tyrosine 65 within the WW domain of PQBP1 is chan…

Available Since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag NEK11

Plasmid

#20546

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

NEK11 (NEK11 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pEBG-MKK4-S255E/T259D (GST)

Plasmid

#21565

Depositor

Leonard Zon

Article

Sanchez et al Nature. . 372(6508):794-8.

Insert

mitogen-activated protein kinase kinase 4 (Map2k4 Mouse)

Tags

GST

Expression

Mammalian

Mutation

Serine 255 to Glutamic Acid, Threonine 259 to As…

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-TRE-DIO-tdTomato-f

Plasmid

#127092

Purpose

An AAV genome with tet-inducible, Cre-dependent expression of farnesylated (f) tdTomato

Depositor

Viviana Gradinaru

Article

Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

Insert

tdTomato-f

Use

AAV

Tags

farnesylation signal from c-Ha-Ras

Expression

Mammalian

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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