We narrowed to 11,604 results for: phen
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Plasmid#59812PurposeAllows the integration of GFP hBub1 in the genome and Tet-inducible expression.DepositorInsertBub1 (BUB1 Human)
TagsEmGFPExpressionMammalianPromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Stargazin-dCherry-FRB
Plasmid#172444PurposeExpression of plasma membrane protein Stargazin tagged with dark (non-fluorescent) mCherry-FRBDepositorAvailable SinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRCPam
Plasmid#195861PurposeEncodes chromogenic protein (CP) with nonsense mutation resulting in amber stop codon at amino acid 62. CP inducible with rhamnose in amber suppressor strains.DepositorInsertAmber Chromogenic Protein on pRCPam plasmid
ExpressionBacterialMutationGln62XAvailable SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti myc-NL-GW
Plasmid#113454PurposeLentiviral NanoLuc Gateway shuttle vector for N-terminal fusionsDepositorTypeEmpty backboneUseLentiviral and LuciferaseTagscmyc-NanoLucExpressionMammalianPromoterhUbCAvailable SinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNSSCP
Plasmid#183188PurposePACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under TDepositorInsertsDivIVA and superfolder GFP fusion protein
SpmX450 and T7 181+ fusion protein
T7 RNAP 1-179 variant and SpmX450 fusion protein
mRFP1 and popZ fusion protein
ExpressionBacterialAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-CYLD-C601A
Plasmid#60028PurposeN-terminal GFP tagged catalytic inactive (C601A) expressionDepositorInsertCylindromatosis (CYLD Human)
TagsGFPExpressionMammalianMutationCysteine 601 changed to AlaninePromoterCMVAvailable SinceNov. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
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HA-MBP-TEVpCS-ADGRE3 CTF-FLAG
Plasmid#217691PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE3 (ADGRE3 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-338Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE2 CTF-FLAG
Plasmid#217690PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE2 (ADGRE2 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-517Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
VanGlow-GL-DSCP
Plasmid#83338Purposefor Gateway cloning of enhancer elements upstream of the DSCP and GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsGFP::Luciferase(nls)ExpressionInsectPromoterDrosophila Synthetic Core PromoterAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHS1801
Plasmid#205966PurposeJAGBLH010000220 Cas8-HNH full system (E. coli codon optimized) in pACYCDuet-1 with Lac promotersDepositorInsertCas8-HNH proteins and CRISPR array
ExpressionBacterialPromoterLac (proteins), pJ23119 (crRNA)Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1232
Plasmid#205969PurposeMWEE01000013 Cas5-HNH operon (E. coli codon optimized) + CRISPR array in pACYCDuet-1 Lac promotersDepositorInsertCas5-HNH proteins and CRISPR array
ExpressionBacterialPromoterLac (proteins), pJ23119 (crRNA)Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 GW-mCit-PA
Plasmid#113449PurposeProteinA-mCitrine Gateway shuttle vector for C-terminal fusionsDepositorTypeEmpty backboneUseGateway shuttle vectorTagsmCitrine-ProteinAExpressionMammalianPromoterCMVAvailable SinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Tight-FLAG-CYLD-C601A
Plasmid#60029PurposeCYLD (C601A) catalytic dead under control of Tet promoter with N-terminal FLAG tagDepositorInsertCylindromatosis (CYLD Human)
TagsFLAGExpressionMammalianMutationCysteine 601 changed to AlaninePromoterTREAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO Myc ArC Long WT
Plasmid#59809PurposeAllows the integration of Myc ArC Long in the genome and Tet-inducible expression.DepositorInsertAurora C (AURKC Human)
TagsMycExpressionMammalianPromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
LBR-FKBP-GFP
Plasmid#222413PurposeTo express LBR with C-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cellsDepositorAvailable SinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL C162F-pRc/CMV
Plasmid#22042DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
TagsHA-tagExpressionMammalianMutationpoint mutant, converting aa 162 from Cysteine to …Available SinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-T-MS2
Plasmid#184839PurposeExpression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.DepositorInsertsingle-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119 and lac promoterAvailable SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGLTR-S-PURO
Plasmid#58244PurposeGATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven Puromycin resistanceDepositorInsertsattR1-Cm(R)-CcdB-attR2
Puro(R)
UseLentiviral and RNAi; Gateway destination vectorExpressionMammalianPromoterSFFV and noAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+pPGI+NAT+tNOS+ku70 insD (SBE217)
Plasmid#195049Purposeintegration cassette for ku70 deletion and expression of NAT gene under pPGI for characterizationDepositorInsertNAT
ExpressionBacterialPromoterpPGIAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only