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pBEL1730

Plasmid

#139921

Purpose

Expression plasmid for LetB mutant K488Bpa

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; K488Amber)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1729

Plasmid

#139920

Purpose

Expression plasmid for LetB mutant F833Bpa

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; F833Amber)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1728

Plasmid

#139919

Purpose

Expression plasmid for LetB mutant F818Bpa

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; F818Amber)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1727

Plasmid

#139918

Purpose

Expression plasmid for LetB mutant Y814Bpa

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; Y814Amber)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1726

Plasmid

#139917

Purpose

Expression plasmid for LetB mutant W476pa

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; W476Amber)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1725

Plasmid

#139916

Purpose

Expression plasmid for LetB mutant F468Bpa

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; F468Amber)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1811

Plasmid

#139915

Purpose

Expression plasmid for LetB mutant L358N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; L359N)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1810

Plasmid

#139914

Purpose

Expression plasmid for LetB mutant L358N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; L358N)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1809

Plasmid

#139913

Purpose

Expression plasmid for LetB mutant L355N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; L355N)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1808

Plasmid

#139912

Purpose

Expression plasmid for LetB mutant V247N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; V247N)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1807

Plasmid

#139911

Purpose

Expression plasmid for LetB mutant L246N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; L246N)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1806

Plasmid

#139910

Purpose

Expression plasmid for LetB mutant L243N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; L243N)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1862

Plasmid

#139909

Purpose

Complementation plasmid for LetAB with LetB mutant L359N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(L359N)

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1861

Plasmid

#139908

Purpose

Complementation plasmid for LetAB with LetB mutant L358N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(L358N)

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1860

Plasmid

#139907

Purpose

Complementation plasmid for LetAB with LetB mutant L355N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(L355N)

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1857

Plasmid

#139904

Purpose

Complementation plasmid for LetAB with LetB mutant L243N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(L243N)

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-R2-pA-L4

Plasmid

#141062

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pExo84-CRU 303

Plasmid

#131165

Purpose

For C-terminal genomic integration of Cub-R-URA3, HIS3 complements the histidine auxotrophy of S. cerevisiae his mutant

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

Insert

ScExo84

Tags

CRU

Expression

Yeast

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAW228

Plasmid

#113242

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader tAggtag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

TTGGTAG->tAggtag

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAW227

Plasmid

#113241

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Atggtag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

TTGGTAG->Atggtag

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge