We narrowed to 14,998 results for: egf
-
Plasmid#140251PurposeCMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-UGI-UGI-P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBE4max(R33A)
ExpressionMammalianMutationR33A in rAPOBEC1, D10A in Cas9PromoterCMVAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSc2
Plasmid#80437PurposeExpresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cellDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertssp Cas9 gRNA
eGFP
UseCRISPRExpressionMammalianPromoterCMV and U6Available sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AB.pCCL.sin.cPPT.U6.miR-145-Decoy.hPGK.GFP.WPRE
Plasmid#46589DepositorAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
MCP-3x9aaTAD
Plasmid#210707PurposeThis Plasmid express EF1a promoter driven MCP-3x 9aa transactivation moduleDepositorInsertUseLentiviralTagsEGFPExpressionMammalianMutationN55K in MCPPromoterEF1aAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB80-hsKIF5B(1-560)-L-GFP
Plasmid#193716PurposeExpression of GFP tagged human kinesin-1 (KIF5B aa1-560)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthsKIF5B(1-560)-L-GFP (KIF5B Human, Synthetic)
TagsEGFPExpressionMammalianMutationhsKIF5B(aa1-560);PromoterChicken beta-actinAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
R711-X12-566: His6-MBP-tev-Hs.NF1iso2 (1203-1530)
Plasmid#159580PurposeE. coli protein expression of His6-MBP-tev-Hs.NF1iso2 (1203-1530)DepositorAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
C1-FT-mCitrine-EAAARx8-CaaX
Plasmid#155224PurposeMPAct with increased search radiusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertF-tractin (aa9-52 of rat ITPKA) fused to mCitrine C-term tagged with codon optimized (EAAAR)x8 and CaaX seq derived from Kras4b
TagsmCitrineExpressionMammalianAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTFE3-donor
Plasmid#112377PurposeCRISPR donor plasmid to tag human transcription factor TFE3 with GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTFE3 homology arms flanking EGFP-IRES-Neo cassette (TFE3 Human)
UseCRISPRMutationhg38:X:49030928:T>A,hg38:X:49030929:A>T,hg3…Available sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-PKI nls
Plasmid#118301PurposeExpression of PKIaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPKI nls (aa 2-36)
TagsFlag and GFPExpressionMammalianMutationinserting 2xnlsAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
WT1 KI gRNA2
Plasmid#92313PurposeCRISPR-GFP-gRNA for cutting WT1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertWT1 (WT1 Human)
TagsCRISPR GFPExpressionMammalianPromoterU6Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pH4R-P2A-mCherry-N1
Plasmid#84333PurposeCo-expresses (untagged) Histamine-4 receptor and mCherryDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH4R-P2A-mCherry (HRH4 Human)
ExpressionMammalianPromoterCMVAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
5HT2C-RNA1-FLAG
Plasmid#79678Purposeserotonin receptor 2C RNA isoform 1 with C-terminal flag tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert5HT2C-RNA1 (HTR2C Human)
TagsflagExpressionMammalianPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRetroX GFP Mps1 DK
Plasmid#63703PurposeInducible expression of GFP-Mps1 DK fusion in mammalian cellsDepositorInsertMps1 (TTK Human)
UseRetroviralTagsEGFPExpressionMammalianMutationdeletion of aa 47-156, kinetochore binding domainPromotertight TRE promoterAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-dSpCas9
Plasmid#201953PurposeMammalian expression plasmid of dead SpCas9 with T2A-EGFP and cloning backbone for sgRNADepositorInsert3xHA-NLS-dSpCas9-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationD10A and H840A on SpCas9PromoterCbhAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pWPT-mCherry
Plasmid#190605PurposeObtained by cloning 5 stop codons in place of the EGFP Kozak sequence in pWPT-/mEGFP-1T-IRES-mCherryDepositorInsertsmEGFP
mCherry
UseLentiviralMutationEGFP Kozak sequence has been replaced with a stre…PromoterEIF1-shortAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-PRMT9 L182A/D183A/I184A/G185A
Plasmid#79676Purposemammalian expression of GFP-PRMT9 catalytically inactive LDIG(182-185)AAAA mutantDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPRMT9 (PRMT9 Human)
TagsEGFPExpressionMammalianMutationL182A/D183A/I184A/G185A catalytic mutantPromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-en15FnCas9
Plasmid#201948PurposeMammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3xHA-NLS-en15FnCas9-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationE1603H on FnCas9PromoterCbhAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLXSN-FLK1 TM
Plasmid#65249PurposeExpression of dominant negative FLK1 in mammalian cellsDepositorInsertFLK1 TM (Kdr Mouse)
UseRetroviralExpressionMammalianMutationdeleted AA 786-1346PromoterLTRAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
CGBE1-VRQR (pBM1192)
Plasmid#140254PurposeCMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteUNG-BE4max(R33A)_VRQR-ΔUGI
ExpressionMammalianMutationR33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …PromoterCMVAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-Aurora_CA
Plasmid#98170Purposeslow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSynthetic construct Aurora_C128A gene
TagsmCherryExpressionMammalianMutationV59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…PromoterCMV (+enhancer)Available sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only