165,744 results

1. pET15b-hnRNP-H

   Plasmid

   #23020

   Depositor

   Douglas Black

   Article

   Chou et al Mol Cell Biol. 1999 Jan . 19(1):69-77.

   Insert

   hnRNP-H (HNRNPH1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   June 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. CBIG-RORb

   Plasmid

   #48709

   Purpose

   bicistronic expression of RORb and EGFP

   Depositor

   Jeffrey Macklis

   Insert

   RORb (Rorb Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CMV/beta-actin

   Available Since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-PfV-Sapphire-IRES-DSRed

   Plasmid

   #116934

   Purpose

   Mammalian lenti vector for expression of the 40nm GEM and DSRed

   Depositor

   Liam Holt

   Article

   Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

   Insert

   Pfv
   

   Use

   Lentiviral

   Tags

   Sapphire

   Promoter

   CMV

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH_SBP-EGFP-GPI

   Plasmid

   #65298

   Purpose

   synchronize trafficking of EGFP-GPI from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   GPI anchor
   

   Use

   Lentiviral

   Tags

   EGFP and IL-2 Signal Sequence and Streptavidin Bi…

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti6MFV SLC25A4

   Plasmid

   #194133

   Purpose

   Expresses ANT1 (SLC25A4) protein in mammalian cells

   Depositor

   Jun Liu

   Insert

   SLC25A4 (SLC25A4 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEC3115 (pSpy0K6-P23_mKate2~*ssrA)

   Plasmid

   #218509

   Purpose

   integrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombination

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   mKate2
   

   Tags

   ssrA degradation tag

   Expression

   Bacterial

   Mutation

   Deletion of Plac_mrfp cassette, introduction of a…

   Promoter

   P23

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOT_5 - lenti-EFS-FMC6.3-28z-2A-puro-2A-tNGFR

   Plasmid

   #181974

   Purpose

   Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

   Insert

   FMC6.3-28z CAR; tNGFR (NGFR Human, Synthetic)
   

   Use

   Lentiviral

   Mutation

   NGFR truncation: aa 11-276

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-21b(+)-Is-PETase-W185A

   Plasmid

   #112204

   Purpose

   pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12

   Depositor

   Gregg Beckham, Christopher Johnson

   Article

   Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

   Insert

   PETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
   

   Tags

   6XHIS

   Expression

   Bacterial

   Mutation

   W185A

   Promoter

   N/A

   Available Since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. mscv2.2

   Plasmid

   #60206

   Purpose

   Mammalian cell expression plasmid; drives constitutive, low-level expression from retroviral LTR; retroviral vector

   Depositor

   Russell Vance

   Article

   Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUE1-TSP-BFLF1

    Plasmid

    #162657

    Purpose

    Expresses BFLF1 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    BFLF1
    

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. FAS promoter luciferase

    Plasmid

    #8890

    Depositor

    Bruce Spiegelman

    Article

    Kim et al J Clin Invest. 1998 Jan 1. 101(1):1-9.

    Insert

    Fatty Acid Synthetase Promoter (Fasn Rat, unknown - rat or mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLC242-GFP-metap2

    Plasmid

    #100518

    Purpose

    Expresses GFP tagged metap2 in mammalian cells to make lentivirus

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    Metap2 (METAP2 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221-SFXN1

    Plasmid

    #132311

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SFXN1 (SFXN1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pAtetO_6xHis-Klentaq

    Plasmid

    #157862

    Purpose

    Plasmid for the aTc-inducible expression of the Klentaq

    Depositor

    Andrew Ellington

    Article

    Milligan et al Biochemistry. 2018 Aug 7;57(31):4607-461

    Insert

    Klentaq
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    tet PA

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC13N-iCAG.copGFP

    Plasmid

    #66578

    Purpose

    CLYBL safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-C13 TALENs

    Depositor

    Jizhong Zou

    Article

    Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

    Insert

    copGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-NES-ZapCV2-Zinc dead

    Plasmid

    #203850

    Purpose

    Expressing zinc dead sensor NES-ZapCV2-Ala in cytoplasm of mammalian cells

    Depositor

    Amy Palmer

    Article

    Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

    Insert

    ZapCV2-Ala zinc dead sensor
    

    Tags

    Nuclear Export Signal

    Expression

    Mammalian

    Mutation

    Cys and His in Zap1 is mutated with Ala

    Promoter

    CMV promoter

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PT2/C-Luc//PGK-SB13

    Plasmid

    #20207

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    Flirefly luciferase/Sb transposase
    

    Use

    Transposon

    Available Since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS1NB c Rosella I

    Plasmid

    #71245

    Purpose

    a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.

    Depositor

    Mark Prescott

    Article

    Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

    Insert

    Rosella
    

    Expression

    Yeast

    Promoter

    PGK1

    Available Since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL3-Basic-IRES

    Plasmid

    #64784

    Purpose

    Luciferase reporter vector

    Depositor

    Joshua Mendell

    Article

    Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

    Type

    Empty backbone

    Use

    Luciferase

    Available Since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNBU2_repA1

    Plasmid

    #211910

    Purpose

    Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.

    Depositor

    Adam Arkin

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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