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pEN_TTmiRc2

Plasmid

#25752

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pAd5-B6/7deltaE3

Plasmid

#175748

Purpose

A block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B6deltaE3 and pAd5-B7.

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Adenovirus 5 genomic region 25043-35938

Use

Adenoviral and Synthetic Biology

Mutation

Adenovirus sequences 27859-30803 replaced with Ba…

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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env8 reverse J1/13

Plasmid

#99831

Purpose

GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

Insert

env8 reverse J1/13

Tags

GFPuv

Expression

Bacterial

Mutation

J1/13 sequence is reversed

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Cmv d0 dPspCas13b-GGS-NYTHDF1

Plasmid

#119855

Purpose

Cmv d0 dPspCas13b-GGS-NYTHDF1 fusion protein (full N-terminal domain of YTHDF1, C-terminal YTH domain omitted)

Depositor

Bryan Dickinson

Article

Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

Insert

dCas13b-GGS-NYTHDF1

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pPRIME-CMV-dsRed-recipient

Plasmid

#11658

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling dsRed expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

dsRed

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pFH2.19_mCherry-MS2x2

Plasmid

#205534

Purpose

Express mCherry-MS2x2 (export tag consisting of 2 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4-Myc-HisA-H248Y-Sirt3

Plasmid

#24917

Depositor

Toren Finkel

Article

Ahn et al Proc Natl Acad Sci U S A. 2008 Sep 23. 1

Insert

Sirt3 (SIRT3 Human)

Tags

His and Myc

Expression

Mammalian

Mutation

His248 mutated to Tyr(Y)

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only
pBP-lldR

Plasmid

#74095

Purpose

L-lactate repressor (LldR) from E. coli MG1655

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

lldR

Use

Synthetic Biology

Expression

Bacterial

Promoter

None

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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MRAS

Plasmid

#55661

Purpose

expression of mouse MRAS

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

MRAS (Mras Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pAC-EsaR

Plasmid

#47643

Purpose

wt esaR gene under control of Plac in pACYC184

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

Plac-esaR-terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plac

Available Since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
pGL3-LRF

Plasmid

#66936

Purpose

Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Forward orientation (attR1, attR2).

Depositor

Alvaro Monteiro

Article

Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Promoter

SV-40

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-rSyntaxin-3a

Plasmid

#195206

Purpose

Mammalian expression vector for GFP tagged Syntaxin-3a.

Depositor

David Bryant, Thomas Weimbs

Article

Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-54

Insert

STX3A (Stx3 Rat)

Expression

Mammalian

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pJAT62

Plasmid

#204301

Purpose

Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

ie1-EGFP

Use

CRISPR

Promoter

ie1

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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OA-1067B

Plasmid

#200252

Purpose

pBac-U6-gRNA(doublesex)-3xp3-tdTomato

Depositor

Omar Akbari

Article

Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

Insert

4 gRNAs targeting Doublesex

Expression

Insect

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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mVenus-PA-PKI

Plasmid

#65456

Purpose

photoactivatable PKA inhibition

Depositor

Klaus Hahn

Article

Yi et al ACS Synth Biol. 2014 Nov 21;3(11):788-95

Insert

PKI (PKIA, Pkia Synthetic)

Expression

Mammalian

Promoter

CMV

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/myc-his Ndel1

Plasmid

#12572

Depositor

Li-Huei Tsai

Article

Shu et al Neuron. 2004 Oct 14. 44(2):263-77.

Insert

Ndel1 (NDEL1 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
hTRIM65_EGFP_N1

Plasmid

#54320

Purpose

Mammalian expression vector for human TRIM65 fusion protein with c-terminal EGFP.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

TRIM65 (TRIM65 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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PfICH pET15b

Plasmid

#60684

Purpose

Recombinant protein expression of Isocyanide Hydratase from Pseudomonas fluorescens

Depositor

Mark Wilson

Article

Lakshminarasimhan et al J Biol Chem. 2010 Sep 17;285(38):29651-6

Insert

inhA

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
hSDH5

Plasmid

#113046

Purpose

Expression of WT hSDH5

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

hSDH5 (SDHAF2 Human)

Expression

Bacterial

Mutation

Insert begins at amino acid 39

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pKL07

Plasmid

#104431

Purpose

mitochondrial matrix targeted super ecliptic pHluorin with C terminal mRuby2 fusion tag in yeast expression vector

Depositor

Joel Kralj

Article

Dodd et al Sci Rep. 2017 Oct 24;7(1):13922. doi: 10

Insert

mito-Super Ecliptic pHluorin-mRuby2

Expression

Yeast

Promoter

Tef1

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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