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Plasmid#50050Purposeexpresses toxoplasma gondii MIC2 extracellular domain in mammalian cellsDepositorInsertMIC2
UseTagsHIS, SBP, and TEVExpressionMammalianMutationS158A, N357S, N463S, N470DPromoterCMVAvailable sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCC1R
Plasmid#213497PurposeEncodes the receptor array and inducible aiiA gene of the three-node Turing circuitDepositorInsertsUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
m2ap
Plasmid#50051Purposeexpresses toxoplasma gondii MIC2 associated protein (m2ap) in mammalian cellsDepositorInsertM2AP
UseTagsHISExpressionMammalianMutationstarts at aa47; N127R, S158A, S177N, N357S, N463S…PromoterCMVAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHelper_NoMutL_V2_gentR
Plasmid#205293PurposeHelper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.DepositorArticleInsertCspRecT / Bxb-1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHelper_Ec1_V1_gentR
Plasmid#205291PurposeHelper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.DepositorArticleInsertCspRecT / Bxb-1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHelper_Ec1_V1_ampR
Plasmid#205292PurposeHelper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.DepositorArticleInsertCspRecT / Bxb-1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM6
Plasmid#49795PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneUseTagsS-tagExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSM3
Plasmid#49799PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneUseTagsS-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDM4
Plasmid#49796PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneUseTagsS-tagExpressionBacterialMutationPromoterT7Available sinceJan. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pL2Cas9
Plasmid#98841PurposeGenome engineering of virulent phagesDepositorInserttracr/Cas9
UseTagsExpressionMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACM4
Plasmid#49797PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneUseTagsS-tagExpressionBacterialMutationPromoterT7Available sinceJan. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCOM4
Plasmid#49798PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneUseTagsS-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMB413a
Plasmid#20058DepositorInsertSperm Whale Myoglobin
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TRP1-PGAL1-GFP (F64L, S65T, V163A)
Plasmid#53206PurposeN-terminal chromosomal tagging with stable GFP under control of GAL promoterDepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsGFP (F64L, S65T, V163A) and TRP1ExpressionMutationPromoterGAL1 promoterAvailable sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1-GFP (F64L, S65T, V163A)
Plasmid#53205PurposeN-terminal chromosomal tagging with stable GFP under control of GAL promoterDepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsGFP (F64L, S65T, V163A) and kanMX6ExpressionMutationPromoterGal1 promoterAvailable sinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMOD4 RT-G
Plasmid#19184DepositorInsertrpsL tetA
UseRecombineeringTags50 nucleotides of FLAG-GFPExpressionMutationPromoterAvailable sinceJan. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-HIS3MX6-PGAL1-GFP (F64L, S65T, V163A)
Plasmid#53207PurposeN-terminal chromosomal tagging with stable GFP under control of GAL promoterDepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsGFP (F64L, S65T, V163A) and HIS3MX6ExpressionMutationPromoterGAL1 promoterAvailable sinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
TK4-NLS-EGFP
Plasmid#239046PurposePiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiationDepositorInsertsEGFP
puroR-T2A-mycNLS-mTagBFP2
UseTagsT2A-mycNLS-mTagBFP2 and c-myc-NLSExpressionMammalianMutationPromoterCAG and EF1aAvailable sinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNitET-SacB-kan
Plasmid#107692PurposeExpression of the phage Che9c RecET system for Recombineering in mycobacteriaDepositorInsertsChe9 phage RecET
PNit2-NitR
SacRB
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKM342
Plasmid#71486PurposeContains loxP-hyg-loxP cassette for recombineering; HygR, AmpR.DepositorInsertloxP-hyg-loxP cassette
UseSource of loxp-hyg-loxp for pcr amplification.TagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only