We narrowed to 26,180 results for: STI
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Plasmid#203423Purposeempty BLINCAR plasmid for payload delivery, no payload, integrates at EGC3 locusDepositorInsertEGC3 plus flanking (EGC3 S. stipitis (yeast))
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CSNK1G3_IDG-K
Plasmid#214460PurposeGateway destination clone of CSNK1G3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CSNK1G3 (CSNK1G3 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CSNK1G1_IDG-K
Plasmid#214458PurposeGateway destination clone of CSNK1G1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CSNK1G1 (CSNK1G1 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-L40A_AnaCNJKFGW_Bba-B0015
Plasmid#202024PurposeExpresses Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant) in E. coliDepositorInsertAnabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant)
UseTagsExpressionBacterialMutationGvpA T6A-L40APromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
XLone-GvpB
Plasmid#173798PurposeMammalian acoustic reporter gene 1 cassette 1 (expressing GvpB)DepositorInsertGvpB
UseTagsmCherry (K187N -please see depositor comments)ExpressionMammalianMutationPromoterTRE3G promoterAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pThuAsol
Plasmid#171675PurposepThuAsol expression in E. coliDepositorInsertSUMO-TEV-ThuAsol
UseTagsHis-tag and SUMO-tagExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
FoxFTVC+bpFOG_MRAS-G22V
Plasmid#107521PurposeConstitutive activation of FGF/MAPK pathway by M-Ras-G22V mutantDepositorInsertM-Ras protein
UseTagsExpressionMutationchanged Glycine 22 to ValinePromoterTVC-specific FoxF enhancer (FoxFTVC+bpFOG)Available sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGH50
Plasmid#107254PurposeVipp1-GFP-FKBP12 construct (spec cassette downstream) used to anchor away Vipp1 (sll0617) of Synecocystis PCC6803.DepositorInsertvipp1-gfp-fkbp12
UseSuicide vector for destined for double homologous…TagsGFP and FKBP12ExpressionBacterialMutationPromoternativeAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/C448Y with 5'UTR
Plasmid#89318Purposeexpression of C448Y spastin M1 and M87 in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M87C448Y
Plasmid#89329PurposeInducible expression of M87 C448Y spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-460 bp deleted, C448YPromoterTet-responsive P tightAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP C448Y with 5'UTR
Plasmid#89325PurposeInducible expression of C448Y spastin M1 and M87 in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutationC448YPromoterTet-responsive P tightAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M1C448Y
Plasmid#89326PurposeInducible expression of M1C448Y spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-194 bp of 5'UTR deleted, C448YPromoterTet-responsive P tightAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
plenti6-SLC7A11/xCT-V5
Plasmid#170427PurposeEepresses SLC7A11 (also named xCT) in mammalian cellsDepositorInsertsolute carrier family 7 member 11 (SLC7A11 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)
Plasmid#185914PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…PromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)
Plasmid#185918PurposepCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337RPromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)
Plasmid#185919PurposepCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…PromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AC 4-μHOM
Plasmid#113619PurposeReporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are induced with the 7a & 7h, or 7a & 7i sgRNAs, with CAS9. EJ using 4 nt of microhomology causes a deletion mutation and restores GFPDepositorInsert4-μHOM variant of EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
I48 ELP
Plasmid#68937PurposeElastin-like polypeptide (VPGxG) with 48 repeats of Isoleucine guest residueDepositorInsertI48 ELP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AC EJ7-GFP
Plasmid#113617PurposeReporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFPDepositorInsertEJ7-GFP variant of EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLIB-HIS-TEV-scCAK1
Plasmid#177014PurposeGenerate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEVDepositorInsertCAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)
UseTagsPolyhistidine-TEVExpressionInsectMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only