We narrowed to 2,970 results for: PPL

1. pC3.1_CAG_oROS-Gr

   Plasmid

   #216113

   Purpose

   Expresses the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.01.31.578117

   Insert

   CAG_oROS-Gr
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMpGE011

   Plasmid

   #71537

   Purpose

   For CRISPR/Cas9 genome editing in Marchantia polymorpha

   Depositor

   Takayuki Kohchi

   Article

   Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_mcherry

   Plasmid

   #167902

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PDLIM4_PDZ_1

   Plasmid

   #103941

   Purpose

   Protein expression and purification of RIL BGLII PDZ domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   PDLIM4 (PDLIM4 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBbdCas9S(RBSmut)_Psyn-sgRNA500

   Plasmid

   #149576

   Purpose

   parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, Psyn

   Available since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EYFP

   Plasmid

   #167898

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. NAX_CAGGS_CAS9_m4_VPR_KanR neo

   Plasmid

   #167885

   Purpose

   PiggyBac compatible plasmid expressing dCas9-VPR

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. mTFP1-Vinculin-23

   Plasmid

   #55516

   Purpose

   Localization: Focal Adhesions, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Insert

   Vinculin (VCL Human)
   

   Use

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pLenti-NG-PE2-BSD

   Plasmid

   #176933

   Purpose

   Expresses PE2-NG variant in mammalian cells

   Depositor

   Hyongbum Kim

   Article

   Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

   Insert

   PE2-NG variant
   

   Use

   Lentiviral

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. BB1_12_pGAP

    Plasmid

    #98498

    Purpose

    BB1 with insert: promoter PGAP

    Depositor

    Diethard Mattanovich, Michael Sauer, Brigitte Gasser

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Insert

    GAP promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Internal BsaI and BpiI sites removed from insert

    Promoter

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. anti-Notch2_B6-pBIOCAM5

    Plasmid

    #39347

    Depositor

    John McCafferty

    Article

    Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

    Insert

    scFv-Fc-fusion specific for Notch2 (clone B6) (NOTCH2 Human)
    

    Use

    Tags

    3xFLAG, 6xHis, and human Fc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pATLIC

    Plasmid

    #110287

    Purpose

    A backbone vector for cell surface display in E. coli by Ligation Independent Cloning (LIC)

    Depositor

    In-Geol Choi

    Article

    Ko et al Appl Environ Microbiol. 2012 May;78(9):3

    Insert

    yfaL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. gRNA-leu-HYB

    Plasmid

    #64332

    Purpose

    leu2 deletion gRNA cassette carried by pRS42H

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    gBlock product of leu2 deletion gRNA cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pDR-GFPuniv

    Plasmid

    #46085

    Purpose

    In vivo recombination assay fluorescent reporter

    Depositor

    Raymond Monnat

    Article

    Li et al Nucleic Acids Res. 2012 Mar;40(6):2587-9

    Insert

    DR-GFPuniv reporter
    

    Use

    Recombination reporter plasmid

    Tags

    Expression

    Mammalian

    Mutation

    E5G and K163R mutations in EGFP relative to wild-…

    Promoter

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. gRNA-his-HYB

    Plasmid

    #64333

    Purpose

    his3 deletion gRNA cassette carried by pRS42H

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    gBlock product of his3 deletion gRNA cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pJM100 (pUC18T-miniTn7T-gm-araC-ParaBAD)

    Plasmid

    #110554

    Purpose

    miniTn7 delivery plasmid with araC-ParaBAD inducible promoter

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    araC-ParaBAD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    AraC-ParaBAD

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. NAX_CAGGS_CAS9_m4_VPR_mcherry

    Plasmid

    #167886

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

18. pLPP5

    Plasmid

    #209967

    Purpose

    Contains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Promoter (P_sppQ)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMpGE006

    Plasmid

    #108722

    Purpose

    Cas9 expression in M.polymorpha

    Depositor

    Shigeo Sugano, Takayuki Kohchi

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Insert

    Atco-Cas9-Pea3ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    MpEFpro

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. H_NES 41kDa

    Plasmid

    #201346

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    HIV_NES-EGFP-2PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pC-KIT5

    Plasmid

    #118987

    Purpose

    Mutational dissection of the G rich region of the c-kit promoter

    Depositor

    Bjorn Hogberg

    Article

    Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

    Insert

    c-kit proto-oncogene {promoter} (KIT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutK2-mutSP-K1

    Promoter

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEasyCas9

    Plasmid

    #184909

    Purpose

    Expresses the Streptococcus pyogenes Cas9 protein

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLV U6-sgRNA UbC-tdTomato

    Plasmid

    #106949

    Purpose

    sgRNA cloning cassette using BsmBI/Esp3I enzymes with tdTomato marker

    Depositor

    Albert Edge

    Article

    Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

    Insert

    sgRNA BsmBI/Esp3I cloning site
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMpGE_En01

    Plasmid

    #71534

    Purpose

    Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

    Depositor

    Takayuki Kohchi, Ikuko Hara-Nishimura

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRS315-ADH700p-yeCherry-p150-yeGFP-CYCt

    Plasmid

    #194519

    Purpose

    Dual fluorescence reporter for studying protein degradation

    Depositor

    Tommer Ravid

    Article

    Mashahreh et al Nat Commun. 2022 Dec 8;13(1):7588. doi:

    Insert

    TIF4631 leader sequence
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKB01-PssbB-mKate2

    Plasmid

    #69105

    Purpose

    Expresses mKate2 (codon adapted for Streptococcus pneumoniae); under control of CSP inducible promoter PssbB. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

    Depositor

    Jan-Willem Veening

    Article

    Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

    Insert

    mKate2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pC-KIT1

    Plasmid

    #118983

    Purpose

    Mutational dissection of the G rich region of the c-kit promoter

    Depositor

    Bjorn Hogberg

    Article

    Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

    Insert

    c-kit proto-oncogene {promoter} (KIT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. L_NES 41kDa

    Plasmid

    #201347

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    Adeno_NES-EGFP-2PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA-Stra6L-6His

    Plasmid

    #174215

    Purpose

    Stra6L-6His (N-Terminal) fusion protein driven by an CMV promotor. May be used to study the murine Vitamin A receptor Stra6L.

    Depositor

    Adrian Press

    Article

    Stafast et al ACS Appl. Polym. Mater. 2022

    Insert

    Stra6L (Stra6l Mouse)
    

    Use

    Tags

    6-His

    Expression

    Mammalian

    Mutation

    non

    Promoter

    CMV

    Available since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRAN473

    Plasmid

    #120813

    Purpose

    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target protein

    Depositor

    Craig Ellermeier, David S. Weiss

    Article

    Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1

    Insert

    red fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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