Skip to main content
Addgene
Showing: 2701 - 2750 of 4301 results

  5. pET28a(+)::NL3F10H

    Plasmid
    #141290
    Purpose
    NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Article
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    NLUC3F10H
    Use
    Luciferase
    Tags
    Flag (x3) and His (x10)
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    141290-plasmid-map-sequence-id-324909

    Enlarge

  30. LUC56 (Luc/UGA/SP)

    Plasmid
    #112147
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Article
    Shetty et al J Biol Chem. 2014 Sep 5;289(36):25317-26
    Insert
    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type SELENOP 3'UTR (Selenop Photinus pyralis, Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Cysteine 258mutated to selenocysteine (U)
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112147-plasmid-map-sequence-id-214894

    Enlarge

  31. IRE-nonOpt-TREAT

    Plasmid
    #196922
    Purpose
    The codon optimized Renilla coding sequence in the IRE-TREAT reporter was replaced by non-optimal Renilla containing 93 synonymous codon substitutions.
    Depositor
    Jeffrey Chao
    Article
    Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.
    Insert
    non-optimal Renilla luciferase
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196922-plasmid-map-sequence-id-387473

    Enlarge

  34. pGL3_4xUAS_CP-candidate_luc+

    Plasmid
    #125154
    Purpose
    STAP-seq luciferase validation vector, which harbors an 4x UAS array upstream of the CP position (candidates are inserted at CP position) for the recruitement of GAL4-DBD tagged proteins
    Depositor
    Alexander Stark
    Article
    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:
    Insert
    4 x upstream activating sequence (UAS)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    125154-plasmid-map-sequence-id-244438

    Enlarge

  48. pNB785

    Plasmid
    #60164
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Use
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    SIC1pr
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60164-plasmid-map-sequence-id-96373

    Enlarge

Showing: 2701 - 2750 of 4301 results