We narrowed to 4,495 results for: Ado

1. EF1a_SIX2_P2A_Hygro_Barcode

   Plasmid

   #120477

   Purpose

   Barcoded lentiviral vector to express SIX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   SIX2 (SIX2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDNR-SpCas9

   Plasmid

   #80425

   Purpose

   Used for in vitro transcription of SpCas9 mRNA.

   Depositor

   Sara Howden, Melissa Little

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   none

   Expression

   Mutation

   codon optimized for human

   Promoter

   T7

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-Map7-fulllength

   Plasmid

   #46076

   Depositor

   Edgar Gomes

   Article

   Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

   Insert

   Map7-fulllength (Map7 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2 hSmad1-EVE

   Plasmid

   #22993

   Depositor

   Edward De Robertis

   Article

   Fuentealba et al Cell. 2007 Nov 30. 131(5):980-93.

   Insert

   Smad1 (SMAD1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S463E, S465E

   Promoter

   Available Since

   June 7, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-TRE-DIO-mTurquoise2

   Plasmid

   #99115

   Purpose

   An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mTurquoise2

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   DIO-mTurquoise2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. MBD-IFP2.0C

   Plasmid

   #215748

   Purpose

   Expresses MBD1 MBD domain fused to C-terminal part of IFP2.0 for 5mC detection in BiAD sensors

   Depositor

   Albert Jeltsch

   Article

   Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

   Insert

   IFP2.0C
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. MBD_R44Q-IFP2.0C

   Plasmid

   #215749

   Purpose

   Expresses binding-deficient R44Q mutant of MBD1 MBD domain fused to C-terminal part of IFP2.0 as negative control for 5mC detection in BiAD sensors

   Depositor

   Albert Jeltsch

   Article

   Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

   Insert

   IFP2.0C
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. 2xHP1bCD-IFP2.0C

   Plasmid

   #215750

   Purpose

   Expresses double domain of HP1beta chromo domain fused to C-terminal part of IFP2.0 for H3K9me2/3 detection in BiAD sensors

   Depositor

   Albert Jeltsch

   Article

   Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

   Insert

   2xHP1bCD-IFP2.0C
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBOB-CARMIL2 BH domain-GFP

   Plasmid

   #118736

   Purpose

   To express CARMIL2 BH tagged with GFP on C-terminus

   Depositor

   John Cooper

   Article

   Lanier et al J Biol Chem. 2016 Jan 15;291(3):1076-91.

   Insert

   CARMIL2 BH domain (CARMIL2 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-tLuc-mCherry

    Plasmid

    #107553

    Purpose

    tLuc-mCherry in AAV backbone

    Depositor

    Juan Belmonte

    Article

    Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas9-beta

    Plasmid

    #169240

    Purpose

    Cas9 and beta expression plasmid for performing gene knockouts via oligo recombineering

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Inserts

    cas9
    beta
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAC-aZEA

    Plasmid

    #53286

    Purpose

    Contains crtE, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, crtI gene of Rhodobacter capsulatus, and lcyE cDNA of Arabidopsis thaliana and produces alpha-zeacarotene in E. coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    lcyE (LUT2 Mustard Weed)
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    lac

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLPCX2-FLAG-Smad4

    Plasmid

    #89834

    Purpose

    Express Smad4 in mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

    Insert

    SMAD4 (SMAD4 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-HA-β2AR-NNES-NanoLuc-15aa linker-AsLOV2(V416L)

    Plasmid

    #125224

    Purpose

    Expresses β2AR-NanoLuc-AsLOV2(V416L) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    HA-β2AR-NNES-NanoLuc-15aa linker-AsLOV2(V416L)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCS2 hSmad1-EVE-MM

    Plasmid

    #22994

    Depositor

    Edward De Robertis

    Article

    Fuentealba et al Cell. 2007 Nov 30. 131(5):980-93.

    Insert

    Smad1 (SMAD1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S214A, S206A, S195A, S187A, S463E, S465E

    Promoter

    Available Since

    June 7, 2010

    Availability

    Academic Institutions and Nonprofits only

16. EF1a_SPIB_P2A_Hygro_Barcode

    Plasmid

    #120483

    Purpose

    Barcoded lentiviral vector to express SPIB in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    SPIB (SPIB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. SB-SRT-Puro

    Plasmid

    #154888

    Purpose

    Sleeping Beauty self-reporting transposon with puromycin reporter

    Depositor

    Rob Mitra, Joseph Dougherty

    Article

    Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

    Insert

    Sleeping beauty SRT
    

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. NylB'-SCY_pET21b

    Plasmid

    #215417

    Purpose

    Expression construct for NylB'-SCY gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylB'-SCY
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R187S/F264C/D370Y

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAGK112

    Plasmid

    #228127

    Purpose

    Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) HEK4 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1531 split retron ncRNA and gRNA to edit HEK4 gene (SNP + PAM recode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available Since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS2 hSmad1-GM

    Plasmid

    #22992

    Depositor

    Edward De Robertis

    Article

    Fuentealba et al Cell. 2007 Nov 30. 131(5):980-93.

    Insert

    Smad1 (SMAD1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S210A ,T202A, S191A, S198A

    Promoter

    Available Since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only