Addgene

We narrowed to 14,941 results for: SHE

Showing: 1081 - 1100 of 14941 results

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pU6-BoxB-petracrRNA

Plasmid

#207625

Purpose

Expresses BoxB-petracrRNA in mammalian cells (with a human-U6 promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

BoxB-petracrRNA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-msEGFP1.8

Plasmid

#208619

Purpose

Mammalian expression of a EGFP variant. Mutations were adopted from (Addgene#135301, PMC7508230), named msEGFP1.8 because N-ter and C-ter features of that in msGFP2 (PMC7508230) not included.

Depositor

Quanfu Ma

Insert

msEGFP1.8

Use

Tags

Expression

Mammalian

Mutation

(compared with pEGFP-C1, protein level) S30R, Y39…

Promoter

CMV

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLPhygSaCas9

Plasmid

#123262

Purpose

Expresses Staphylococcus aureus Cas9 (SaCas9) in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

Insert

Staphylococcus aureus Cas9 (NEWENTRY )

Use

CRISPR; Leishmania

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSPneoSagRNAH

Plasmid

#123265

Purpose

Expresses gRNA of Staphylococcus aureus Cas9 (SaCas9) in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

Type

Empty backbone

Use

CRISPR; Leishmania expression

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pNeoSagRNAH

Plasmid

#123266

Purpose

Expresses gRNA of Staphylococcus aureus Cas9 (SaCas9) in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

Type

Empty backbone

Use

CRISPR; Leishmania expression

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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IgH P+ VA20

Plasmid

#127249

Purpose

IgH V-region

Depositor

Christine Milcarek

Article

Shell et al J Immunol. 2007 Dec 1;179(11):7663-73.

Insert

Promoter+V-region of IgH of A20 B cell line

Use

Other

Tags

Expression

Mutation

Promoter

IgH

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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KFP14

Plasmid

#119796

Purpose

RpoD6 epitope tagged construct for integration at neutral site 1

Depositor

Erin O'Shea

Article

Fleming et al Cell Rep. 2018 Dec 11;25(11):2937-2945.e

Insert

Synpcc7942_1557 (Synpcc7942_1557 )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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KFP13

Plasmid

#119795

Purpose

RpoD5 epitope tagged construct for integration at neutral site 1

Depositor

Erin O'Shea

Article

Fleming et al Cell Rep. 2018 Dec 11;25(11):2937-2945.e

Insert

Synpcc7942_1849 (Synpcc7942_1849 )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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KFP06

Plasmid

#119799

Purpose

RpoD6 epitope tagged construct for integration at neutral site 2.2

Depositor

Erin O'Shea

Article

Fleming et al Cell Rep. 2018 Dec 11;25(11):2937-2945.e

Insert

Synpcc7942_1557 (Synpcc7942_1557 )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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KFP05

Plasmid

#119800

Purpose

RpoD5 epitope tagged construct for integration at neutral site 2.2

Depositor

Erin O'Shea

Article

Fleming et al Cell Rep. 2018 Dec 11;25(11):2937-2945.e

Insert

Synpcc7942_1849 (Synpcc7942_1849 )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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P0050::rpaAD53A

Plasmid

#101831

Purpose

drives expression of rpaA D53A variant from an rpaA-independent promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

RpaA D53A with p0050 promoter (+ gentamicin resistance casette)

Use

Cloning vector

Tags

Expression

Mutation

D53 mutated to A

Promoter

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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human pirh2-(1-137)

Plasmid

#24879

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

Pirh2 (RCHY1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Contains aa 1-137

Promoter

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only
human pirh2-(1-195)

Plasmid

#24880

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

Pirh2 (RCHY1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Contains aa 1-195.

Promoter

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only
pJRL2 GAL1(TATA-A1T6)pr VYFP (EB#1573)

Plasmid

#14849

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

GAL1 promoter TATA-A1T6 (GAL1 Budding Yeast)

Use

Tags

VYFP

Expression

Yeast

Mutation

TATA-A1T6. TATA box mutated from TATATAAG to AATA…

Promoter

Available since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
pJRL2 GAL1(TATA-A1T6)pr ECFP (EB#1574)

Plasmid

#14850

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

GAL1 promoter TATA-A1T6 (GAL1 Budding Yeast)

Use

Tags

ECFP

Expression

Yeast

Mutation

TATA-A1T6. TATA box mutated from TATATAAG to AATA…

Promoter

Available since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
CaMCLC-His

Plasmid

#111292

Purpose

bacterial expression

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

(S)-malyl-CoA/b-methylmalyl-CoA/(S)-citramalyl- CoA lyase

Use

Tags

His tag, GGGSGGGSLEHHHHHH

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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MsMUT_sgRNA1

Plasmid

#111296

Purpose

CRISPR KO murine MUT

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

sgRNA1 against murine MUT

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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MsMUT_sgRNA3

Plasmid

#111297

Purpose

CRISPR KO murine MUT

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

sgRNA3 against murine MUT

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pBAD-Lime

Plasmid

#191255

Purpose

Bacterial expression plasmids for pH sensitive fluorescent protein Lime

Depositor

Robert Campbell

Article

Shen et al ACS Sens. 2023 Aug 25;8(8):3014-3022. do

Insert

Lime

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-GFP

Plasmid

#171123

Purpose

Doxycycline inducible expression of GFP

Depositor

Jason Sheltzer

Article

Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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