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pcDNA3.1-CHRFAM7ADelta2bp-mCherry

Plasmid

#62638

Purpose

mammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with mCherry fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Use

Tags

Expression

Mammalian

Mutation

there is a 2-bp deletion in exon 6 of CHRFAM7A an…

Promoter

CMV

Available since

March 10, 2015

Availability

Academic Institutions and Nonprofits only
pLVX-SV40-Galpha-13 ONE-GO

Plasmid

#204332

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

SV40 Galpha-13 ONE-GO (GNA13 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pJOG252

Plasmid

#80584

Purpose

recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:nptII-tnos
p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pJOG253

Plasmid

#80585

Purpose

recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:PAT-tnos
p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pET-20b-T7-Ntx-K49

Plasmid

#58715

Purpose

Expresses in Escherichia coli T7-Notexin with the amino acid substitution D49K

Depositor

Fiorella Tonello

Article

Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

Insert

Notexin

Use

Tags

T7 peptide tag

Expression

Bacterial

Mutation

Changed Aspartic Acid 49 to Lysine

Promoter

T7

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-s ONE-GO

Plasmid

#189731

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-s ONE-GO
GNAS (GNAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/FGFR2myc

Plasmid

#33248

Depositor

Alka Mansukhani, Claudio Basilico

Article

Mansukhani et al J Cell Biol. 2000 Jun 12. 149(6):1297-30

Insert

FGFR2 (Fgfr2 Mouse)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

KpnI site introduced at stop codon of FGFR2 to be…

Promoter

CMV

Available since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only
pNK5867

Plasmid

#219751

Purpose

pGAP-like vector encoding hispidin-synthase from Mycena citricolor under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - mcitHispS - tAOX

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-hSyn-Galpha-s ONE-GO

Plasmid

#214371

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Galpha-s ONE-GO (GNAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hSyn

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pNK3203

Plasmid

#219749

Purpose

DVK_AF CIDAR vector encoding hispidin-synthase from Mycena citricolor under control of CMV promoter, for mammalian expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pCMV - mcitHispS - tSV40

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-SV40-Galpha-s ONE-GO

Plasmid

#204327

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

SV40 Galpha-s ONE-GO (GNAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRFAM7ADelta2bp

Plasmid

#62515

Purpose

mammalian expression of human duplicated Alpha7 with 2 bp deletion (CHRFAM7A-∆2bp)

Depositor

Sherry Leonard, Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7ADelta2bp (CHRFAM7A Human)

Use

Tags

Expression

Mammalian

Mutation

partially duplication of CHRNA7 with 2-bp deletio…

Promoter

CMV

Available since

March 20, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pMDC32B-NbmiR482aTS-B/c

Plasmid

#227967

Purpose

Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. For expressing syn-tasiRNAs in Nicotiana benthamiana.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Inserts

B/c
NbmiR482aTS

Use

Tags

Expression

Plant

Mutation

Promoter

35S and None

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRFAM7ADelta2bp-YFP

Plasmid

#62637

Purpose

mammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with YFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Use

Tags

Expression

Mammalian

Mutation

there is a 2-bp deletion in exon 6 of CHRFAM7A an…

Promoter

CMV

Available since

March 10, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/FGFR2-S252W(Apert) myc

Plasmid

#33249

Depositor

Alka Mansukhani, Claudio Basilico

Article

Mansukhani et al J Cell Biol. 2000 Jun 12. 149(6):1297-30

Insert

FGFR2 (Fgfr2 Mouse)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Mutation S252W in FGFR2 found in Apert syndrome. …

Promoter

CMV

Available since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only
delta-BRIP1-pcDNA3.1/MycHis

Plasmid

#19042

Depositor

Ronny Drapkin

Article

De Nicolo et al Clin Cancer Res. 2008 Jul 15. 14(14):467

Insert

delta BRIP1 (BRIP1, BRCA1 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

This is a novel BRIP1 germline mutation that cons…

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
FGFR2 3xFlag

Plasmid

#110063

Purpose

FGFR2 expression in mammalian cells (JCOPCO paper)

Depositor

Martin Fernandez-Zapico

Article

Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

Insert

fibroblast growth factor receptor 2 (FGFR2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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FGFR2 F276C 3xFlag

Plasmid

#110064

Purpose

FGFR2 F276C expression in mammalian cells (JCOPO paper)

Depositor

Martin Fernandez-Zapico

Article

Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

Insert

fibroblast growth factor receptor 2 (FGFR2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

mutated Phenylalanine 276 to Cysteine (F276C)

Promoter

CMV

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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FGFR2 K41E 3xFlag

Plasmid

#110105

Purpose

expresses FGFR2 with a single amino acid change in mammalian cells

Depositor

Martin Fernandez-Zapico

Article

Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

Insert

fibroblast growth factor receptor 2 (FGFR2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

mutated Lysine 41 to Glutamic acid (K41E)

Promoter

CMV

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pPtPBR2

Plasmid

#80387

Purpose

Episome vector for Phaeodactylum tricornutum (pBR322-based)

Depositor

Philip Weyman

Type

Empty backbone

Use

Synthetic Biology; ; episomal vector for phaeodac…

Tags

Expression

Mutation

Promoter

Available since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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