We narrowed to 15,323 results for: pET

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CyPet-FKBP-E2

Plasmid

#175244

Purpose

Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

UBE2I (UBE2I Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET15b-ITPA

Plasmid

#60824

Purpose

to purify human ITPA from bacteria

Depositor

Youri Pavlov

Article

Porta et al Acta Crystallogr Sect F Struct Biol Crys

Insert

ITPA (ITPA Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pET31b-T7-Spinach2-Pl-B

Plasmid

#79161

Purpose

Expresses the Pl-B biosensor under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

T7-tRNA-Spinach2-Pl-B

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pet11a-ABLE

Plasmid

#158627

Purpose

Amp resistant IPTG inducible 6xHis-TEV-ABLE protein for E. coli expression

Depositor

William DeGrado

Article

Polizzi et al Science. 2020 Sep 4;369(6508):1227-1233.

Insert

Apixaban-Binding Helical Bundle

Tags

6x His-tag and TEV cleavage tag

Expression

Bacterial

Promoter

T7

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a Gx-NL2

Plasmid

#169107

Purpose

Expresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

Depositor

Maarten Merkx

Article

Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

Insert

protein G - NanoLuc - NanoLuc

Tags

his-tag and strep-tag

Expression

Bacterial

Mutation

Ala 24 stop

Promoter

T7

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pET31b-T7-Spinach2-Pl-A

Plasmid

#79160

Purpose

Expresses the Pl-A biosensor under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

T7-tRNA-Spinach2-Pl-A

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pET26-CNTnw

Plasmid

#100163

Purpose

recombinant expression of target protein as an MBP-His tag fusion at the N-terminus of target protein

Depositor

Seok-Yong Lee

Article

Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

Insert

Concentrative nucleoside transporter

Tags

MBP-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-BCA-P(11)4

Plasmid

#185780

Purpose

Encodes enzyme bovine carbonic anhydrase linked to P(11)4 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

BCA-P(11)4

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2353_Tier3(PB)-YPet_PuroR

Plasmid

#169652

Purpose

Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PRPBSA-driven YPet and PuroR expression

Expression

Mammalian

Promoter

PRPBSA

Available Since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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CYPet-H2B-6

Plasmid

#55461

Purpose

Localization: Nucleus/Histones, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Insert

H2B (H2BC11 Human)

Tags

CYPet

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pET-CV-VAMP2

Plasmid

#168023

Purpose

Expresses eCFP-VAMP2(T27-R86)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-VAMP2(T27-R86)-mVenus

Tags

Hisx6

Expression

Bacterial

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a - SodB

Plasmid

#89613

Purpose

Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase B protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Superoxide dismutase B

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pET16b-Cas2

Plasmid

#122286

Purpose

Cas2 cloned in pET16b

Depositor

Jennifer Doudna

Article

Nunez et al Nat Struct Mol Biol. 2014 Jun;21(6):528-

Insert

Eco Cas2

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET21b-RL016A

Plasmid

#42135

Depositor

Sheref Mansy

Article

Lentini et al ACS Synth Biol. 2013 Mar 15.

Insert

mYPet-mCherry

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 20, 2013

Availability

Academic Institutions and Nonprofits only

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pET28a-T5-ASG_ClpXP

Plasmid

#153299

Purpose

Expresses acoustic sensor genes or ClpXP-sensitive gas vesicles in E. coli Nissle 1917

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

ASG

Use

Synthetic Biology

Expression

Bacterial

Promoter

T5

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pET23(a)_mod

Plasmid

#26363

Depositor

Thomas Tuschl

Article

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Insert

FLAG/HA

Expression

Bacterial

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pET15b+PCNA1 WT

Plasmid

#66139

Purpose

Expresses the wild type of Sulfolobus solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 1

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pET-UbcH10-DN

Plasmid

#8502

Depositor

Joan Ruderman

Article

Bastians et al Mol Biol Cell 1999 Nov;10(11):3927-41.

Insert

UbcH10 (UBE2C Human)

Tags

His

Expression

Bacterial

Mutation

Dominant Negative, C114S

Available Since

June 1, 2005

Availability

Academic Institutions and Nonprofits only
pET28b-AF0623

Plasmid

#30164

Depositor

SeWon Suh

Article

Kim do et al Acta Crystallogr Sect F Struct Biol Crys

Insert

AF0623 (lig Archaeoglobus fulgidus)

Tags

His

Expression

Bacterial

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only
pETM6-H9-mCherry

Plasmid

#66532

Purpose

Expresses mCherry under the control of Mutant T7 Promoter H9

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon Optimized for E. coli

Promoter

Mutant T7 Promoter - H9

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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