We narrowed to 5,516 results for: pcas

1. pCAG-EGFP-C-IFT81

   Plasmid

   #218732

   Purpose

   Expresses N-terminally EGFP-tagged IFT81 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Ishida et al Mol Biol Cell. 2022 Aug 1;33(9):ar83. do

   Insert

   IFT81 (IFT81 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. U6-hGRIN2B-CAG-ps-SpCas9

   Plasmid

   #102851

   Purpose

   A single-chain light-controllable dSpCas9 with pdDronpa1 domains for hGRIN2B gene editing

   Depositor

   Michael Lin

   Article

   Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

   Insert

   sp-hGRIN2B-sgRNA; dSpCas9; pdDronpa1 (GRIN2B S. pyogenes, Synthetic, Human)
   

   Use

   CRISPR

   Tags

   3X Flag and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D

   Plasmid

   #110302

   Purpose

   Expresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1

   Depositor

   Chris Kok-Lung Chan

   Article

   Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

   Insert

   p53-binding protein 1 (TP53BP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-3xFLAG-Rbfox3

   Plasmid

   #213727

   Purpose

   Mammalian cell expression vector for overexpression of Rbfox3 in motor neurons.

   Depositor

   Chaolin Zhang

   Article

   Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

   Insert

   Rbfox3 (Rbfox3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. NM9-SpCas9-NLS3

   Plasmid

   #128177

   Purpose

   Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

   Depositor

   Huimin Zhao

   Article

   Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

   Insert

   SpCas9-NLS3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pPGK1

   Available since

   April 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpCas9-YE1-BE4max

   Plasmid

   #198546

   Purpose

   Expresses human codon-optimized SpCas9-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-YE1-BE4max-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-YE1-BE4max
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-3xFLAG-Rbfox1

   Plasmid

   #213725

   Purpose

   Mammalian cell expression vector for overexpression of Rbfox1 in motor neurons.

   Depositor

   Chaolin Zhang

   Article

   Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

   Insert

   Rbfox1 (Rbfox1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-cofilin-S3A

   Plasmid

   #78296

   Purpose

   Mammalian expression of mouse cofilin S3A (nonphosphorylatable form) fused to EGFP

   Depositor

   Michael Frotscher

   Article

   Chai et al Development. 2016 Mar 15;143(6):1029-40.

   Insert

   cofilin 1 (Cfl1 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S3A

   Promoter

   CAG

   Available since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-nucGFP11-3xControlgRNA

   Plasmid

   #224568

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   GFP11-H2B
   

   Use

   CRISPR

   Tags

   Histone H2B (nuclear localization)

   Expression

   Mutation

   Codon optimized

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDNR-SpCas9-Gem

    Plasmid

    #80424

    Purpose

    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining

    Depositor

    Melissa Little, Sara Howden

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9-Gem
    

    Use

    CRISPR

    Tags

    Geminin

    Expression

    Mutation

    codon optimized for human

    Promoter

    T7

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-EGFP-C-IFT74

    Plasmid

    #218730

    Purpose

    Expresses N-terminally EGFP-tagged IFT74 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Nishijima et al Mol Biol Cell. 2017 Jun 15;28(12):1652-1

    Insert

    IFT74 (IFT74 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG-EGFP-C-IFT70B

    Plasmid

    #218729

    Purpose

    Expresses N-terminally EGFP-tagged IFT70B in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Takei et al Biol Open. 2018 Apr 30;7(5). pii: bio.03

    Insert

    IFT70B (TTC30B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-EGFP-C-IFT52

    Plasmid

    #218725

    Purpose

    Expresses N-terminally EGFP-tagged IFT52 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Takei et al Biol Open. 2018 Apr 30;7(5). pii: bio.03

    Insert

    IFT52 (IFT52 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-HkCas12a-2AeGFP

    Plasmid

    #123634

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    HkCas12a
    

    Use

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-HA-Nrxn1beta AS4(-)

    Plasmid

    #59409

    Purpose

    Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]

    Depositor

    Peter Scheiffele

    Article

    Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

    Insert

    Nrxn1beta AS4(-) (Nrxn1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-mCherry2-mito

    Plasmid

    #164164

    Purpose

    Expresses mCherry2-fused with a mitochondria-targeting signal of the ActA gene of Listeria monocytogenes under the control of CAG promoter

    Depositor

    Isei Tanida

    Article

    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

    Insert

    mCherry2-mtActA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS-SspB-mScarlet-I

    Plasmid

    #178521

    Purpose

    Expresses SspB-mScarlet-I in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    SspB-mScarlet-I
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG tubulin GFP

    Plasmid

    #66105

    Purpose

    expression of chicken tubulin GFP

    Depositor

    Martin Scaal, Felicitas Proels

    Article

    et al Development. 2015 Feb 15;142(4):665-71.

    Insert

    tubulin
    

    Use

    Chicken expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

19. NLS-SpCas9mSA-His

    Plasmid

    #182951

    Purpose

    Express SpCas9mSA in bacteria

    Depositor

    Wen Xue

    Article

    Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

    Insert

    NLS-SpCas9mSA-His
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAX-EGFP-CIP4

    Plasmid

    #178363

    Purpose

    Expresses CIP4 c-terminal labeled with EGFP in mammalian cells

    Depositor

    Erik Dent

    Article

    Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

    Insert

    Cdc42 Interacting Protein 4 (TRIP10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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